提莫菲維與野燕麥雜交后代外源遺傳物質(zhì)的檢測(cè).pdf

1、貴州大學(xué)2010屆碩士研究生學(xué)位論文提莫菲維與野燕麥雜交后代外源遺傳物質(zhì)的檢測(cè)學(xué)科專業(yè)：作物遺傳育種研究方向：作物種質(zhì)資源創(chuàng)新研究與利用導(dǎo)師：張素勤教授研究生：盛中飛中國(guó)﹒﹒﹒﹒貴州﹒﹒﹒﹒貴陽(yáng)2010年5月分類號(hào)：S512.6.035.1Q321.3Q343.22論文編號(hào)：2007021118密級(jí)：公開摘要22.2.2.3切片的制作....................................................

2、.................................142.2.3核型分析方法..................................................................................152.2.4染色體C帶................................................................................

3、.....152.2.4.1根尖染色體組切片的制備(同2.2.2)...........................................152.2.4.2染色體C帶實(shí)驗(yàn)步驟..................................................................152.2.5基因組原位雜交..............................................

4、................................162.2.5.1葡萄牙野燕麥與中國(guó)春小麥基因組DNA的提取(CTAB法)及純化(酚氯仿抽提).....................................................................................162.2.5.2DNA檢測(cè)..........................................

5、...........................................162.2.5.3根尖染色體切片的制備(同2.2.2)...............................................172.2.5.4DNA片段的酶切.........................................................................172.2.5.5合成探針

6、.......................................................................................172.2.5.6雜交混合液的配制.......................................................................172.2.5.7基因組原位雜交和圖像分析........................

7、...............................18第三章結(jié)果與分析..................................................................................................203.1親本及雜交后代的形態(tài)特征................................................................

8、.....203.1.1親本與雜交后代的種子形狀..........................................................203.1.2雜種后代田間性狀及產(chǎn)量性狀調(diào)查..............................................203.1.3親本及雜種后代田間抗病性調(diào)查.................................................

9、.213.2核型分析.....................................................................................................213.2.1提莫菲維小麥的核型分析..............................................................213.2.2葡萄牙野燕麥的核型分析...........

10、...................................................223.2.3提莫菲維小麥與葡萄牙野燕麥雜種后代株系的核型分析..........243.2.3.1TP1株系的核型分析...................................................................243.2.3.2TP2株系的核型分析.......................

11、............................................253.2.3.3TP3株系的核型分析...................................................................273.2.3.4TP4株系的核型分析...................................................................283.

12、2.3.5單體后代1的核型分析...............................................................303.2.3.6單體后代2的核型分析.............................................................313.2.3.7雙單體后代的核型分析...........................................

13、......................333.2.4雜交后代與親本核型的比較分析..................................................343.2.5雜交后代與親本進(jìn)化系數(shù)的比較分析..........................................393.3染色體C帶分析..................................................

14、......................................403.3.1提莫菲維小麥的染色體C帶.........................................................403.3.2葡萄牙野燕麥染色體C帶.............................................................413.3.3雜交后代株系的染色體C帶........