陽離子熒光共軛聚合物的合成及其在生物分子檢測中的應用.pdf

1、湖南大學碩士學位論文陽離子熒光共軛聚合物的合成及其在生物分子檢測中的應用姓名：王勝鋒申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：羊小海王柯敏20080601AbstractAsanewclassofwater—solublefluorescentmolecules，cationicfluorescentconjugatedpolymershaveanumberofadvantagessuchashighabsorptionco

2、efficientsandhighfluorescencequantumyieldsAtthesametime，conjugatedpolymersareprovidedwithmolecularwireeffect，andcanamplifyfluorescencesignalBecauseoftheseuniquefluorescenceproperties，cationicfluorescentconjugatedpolymers

3、haveverywideapplicationprospectInthisthesis，aimingfordeveloplinghighersensitivebiologicaldetectionmethodbasedoncationicfluorescentconjugatedpolymers，threepartsofworkshavebeendemonstratedasfollows：(1)Synthesisofcationicfl

4、uorescentconjugatedpolymerAcationicpolyelectrolytepoly(phenyleneethynylene)derivative(CCP)wassynthesizdbySonogashiraHagiharacouplingreaction，becausesidechainsoftheconjugatedpolymercontainedalotofhydroxylgroupsandaminegro

5、ups，theCOnjugatedpolymerhasexcellentwatersolubilityThemaximumexcitationandemissionwavelengthwere430nmand497nmrespectivelyAtthesametime，thefluorescencequantumoftheconjugatedpolymerwasmensuratedwithRhodamine6Gasreferencean

6、ditreached023Thedatashowedtheconjugatedpolymerhadahighfluorescencequantumyield(2)ThestudyonproteindetectionutilizingcationicfluorescentconjugatedpolymerandaptamerprobeAsimple，sensitivemethodofproteindetectionwasdeveloped

7、basedonconjugatedpolymerasreporter，thrombinastargetprotein，andaptamerprobeasrecognizedmoleculeThequencherlabledaptamerprobeattachedtoCCPbyelectrostaticinteraction，leadingtofluorescencequenchingofCCP；whenthrombinadded，itb

8、indedspecificallytoaptamerprobe，whichmadetheCCPreleased，thenthefluorescenceofCCPwasresumedThefluorescenceresumingratiowasproportionaltotheconcentrationoftargetproteinAfteraseriesofinvestigationandoptimizationonexperiment

9、alconditions，thequantitativedetectionofthrombinwasachievedinaqueoussolution，andthedetectionlimitwas17nM，thenthespecificityofthemethodwasverifiedThismethodonlyrequiressimplemodificationofaptamer，andthespecificityofaptamer