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1、UniversityCode:10225RegisterCode:080524DissertationfortheDegreeofMasterTheStudyontwokindsofmolecularmarkeramongthedifferentspeciesofCerasushumilis(Bge)SokandthegeneticvariationofnutritionindicesCandidate:Supervisor:Assoc

2、iateSupervisor:AcademicDegreeAppliedfor:Speciality:DateofOralExamination:University:WangXinAssociateProfAoHongProfWangQiuyuMastergenetics2008623NortheastForestryUniversity目錄AbstractInthisstudythetrialmaterialswereeightki

3、ndsofCerasushumilis(Bge)SokvarietiesfromtheBeijingBureauofForestryseedlingsstationTheCerasushumilis(Bge)SokweredetectedthatthecontentofthesolublesugarandsolubleproteinhadchangedindifferenttimeTheoptimalSRAPPCRandISSRPCRs

4、ystemoftheCerasushumilis(Bge)SokewerefirstestablishedinthisresearchMoreovertheISSRmolecularmarkerWasappliedtostudythegeneticdiversityoftheCerasus辦甜棚玎西(Bge)SokThemainfindingswereasfollows:Theanalyticresultfromeightvarieti

5、esoftheCerasushumilis(Bge)Sokshowedthat61polymorphismsitesCanbeamplifiedfromatotalof80individualsbyl1primers,theproportionofthepolymorphiclociwas7821%,theaverageofeachprimerover55polymorphicsitesThegeneticdiversityofthee

6、ightkindsofCerasus辦甜m玎括(Bge)SokwasestimatedbyShannonindice:TangshanHuairou—labagoumenNo5ofNongdaNo4ofNongdaNo3ofNongdaHuairoutanghekouYanqingjianjiadianHuangshandianThereexistedcertaingeneticdifferentiationinCerasus廳甜,行玎

7、括(Bge)SokThegeneticvariationamongthepopulationswas2532%ofthetotal,andtheother7468%distributedamongindividualswithinpopulationsThisindicatedthatthevariationofCerasushumilis(Bge)SokmainlycamefromwithinpopulationsOnthebasis

8、oftheclusteranalysisofgeneticdistancematrix,theeightkindsofCerasushumilis(Bge)Sokcanbedividedintotwocategories:thegeneticsimilarityamongthevarietiesofHuairoulabagoumen、Yanqingjianjiadian、Huairoutanghekou、No3ofNongda、No4o

9、fNongdaandNo5ofNongdaareobvious;whilethevariationbetweenTangshanandHuangshandianwereenormouswiththeothersThereiSremarkabledifferenceinthesolublesugarcontentamongthedifferentCerasushumilis(Bge)SokvarietiesInthisstudy,thes

10、olublesugarcontentofTangshanandNo3ofNongdaarehigherthanotherkindsofthematdifferentperiods,whiletheNo4ofNongdaandHuangshandianarelowerSimilarlytheresultalsowasconformtothesolubleproteincontentindifferenttimesThesolublepro

11、teincontentWashigher,namely,TangshanandNo3ofNongda,andNo4ofNongdaandHuangshandianWaslowerSo,TangshanandNo3ofNongdawerepreferablevarietiesaccordingtothesolublesugarandsolubleproteincontentindifferentkindsofCerasushumilis(

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