微乳毛細(xì)管電動(dòng)色譜在中藥指紋圖譜中的應(yīng)用.pdf

1、江南大學(xué)碩士學(xué)位論文微乳毛細(xì)管電動(dòng)色譜在中藥指紋圖譜中的應(yīng)用姓名：李楠申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：分析化學(xué)指導(dǎo)教師：曹玉華20080601AbstractAbstractMicroemulsionelectrokineticchromatography(MEEKC)isanewelectrodriVenseparatiOnteclulique，whichwasdeVelopledonthebasisofmicellarelectrokine

2、ticchromatography(MEKC)MEEKChasbeen印pliedtoawiderangeofwatersolubleandinsolublebothchargedandneutralcompounds，whichhasthewidermigrationwindowa11dhigherseparationemciencythanMEKCThisstudywehaVedeVelopedthefinge印rintsofRhe

3、眥inMEEKC，comparedwiththefingerprintsofRheuminHPLC，Clusteringhasthes鋤eresultsessentially，whichshowedthatMEEKCisanothergoodtechniqueintheresearchoffingerprintsofChinesemedicineFirstofall，Inthesystemofmicroemulsionelectroki

4、neticc印illa巧chromatography，theea’ectsofmicroemulsioncompositionsonmigrationwindow，includingthesurfactant，co—surfactant，corephasesaJldorganicmodifierswerestudiedTakingmethanolastheelectroosmoticnowmarkerandphenantllreneas

5、themicroemulsiondropmarker，themigrationwindowwasanalyzedResearchshowedthattheemulsioncompositionofanionicsurfactant(SDS)ando略a11icadditiVe(methnol，acetonitrile)canincreaSethemigrationwindowsignific肌tly，andcosurf如tantnbut

6、aJl01makesthemigrationwindowsmallerinthemeanWhile，theimpactofoilis1essInordertogetgoodsepaurationresults，wecanadjustmemigrationwindowintheestablishmentoft11efingerprimsSecondly，、ⅣehaVedeVelopedthefinge叩rilltsofR加“聊collec

7、tedfromdifreremproducingareaSbyMEEKC，、VhichcanproVideeVidenceforitsquali哆con|croIAuncoatedmsedsilicac?、骯rycolumn(75肛m65cm，the1en舀his55cm)wasused，theO／WmicroemulsionsystemwaSusedasbuffersolutionwhichwascomposeofSDS，n—octa

8、ne，nbutanol，borates01ution(thew／wwaS33％：66％：08％：893％)，atan印pliedvoltageof18kV，ThewaVelengthweredetectedat280衄Byusingclusteranalysisandsimilari夠calculation，the14batchesofrhubarbs鋤plewasclassifledasthreetypes：R砘“聊砌餾w療c“所Ma

9、ximexBalf，theordinaryR辦P“所p口，聊a，“聊LandtheinferiorR辦e“聊pa，聊口，甜聊LThemethodwaSsimpleandreliable，a11ditwascapableofeflfectiVelycontrollingthequalit)，ofrhub缸b，andthisexperimentdeVelopsanewapplicationofMEEKCinfingerprintoftrad

10、itionalChinesemedicineLastly，F(xiàn)ingerprintofImeums鋤plecollectedfromdi虢rentproducingareaswasdeVelopedbyHPLCforitsqualitycontr01TheseparationwaScarriedoutonNucleodurC18(46mm250mm，5Jm)c01硼nJlwithmixturesofO5％phosphateacidmema

11、Jlola11dacetonitrileasmobilephaseinagradientelutionmodel，andthedetectionwavelen釅hwereat280nmByusingclusteranalysisaJldsimil撕tycalculation，the14batchesofrhubarbs鋤pleswereclaSsifiedas3types，theresultismes鋤etotheresultofMEE