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1、AbstraCt中文摘要AbbreviationSListofFiguresListofTablesTableofcontentsChapterIReview：PP2Aanderythropoiesis11PP2Aisaserine／threonjnephosphataseKinasesphosphatasesandPSPsPP2Aholoenzymeholds0comprehensivestructureCellularlocaliz

2、ationandmultistepregulationChemicalinhibitorshumandiseasesandanimalmodels12Hematopoiesisanderythropoiesis—keepinglifegoingonInsightsintohematopoiesis—hotspringfo廠bloodcellsTheb『0logyoferythropoiesiskeepingbloodredReferen

3、cesChapterIhLossofPpp2coalleleinTie2tissuesleadstofetalliveranemiantroductionMaterialsandmethods21EstablishPpp2caconditionalknockoutmice22LossofthePpp2caallelewithinTie2cellsresultsinembryoniclethality23Mutantembryosexhi

4、bitnormalvasculature24PP2Acc￡。deficientembryossufferimpaireddefectivehematopoiesis25AdultmutantmicemanifestnormalhematopoieticparametersDiscussionContributions13578910101214161919232912501231354445555666THESIS：Embryonica

5、nemiainPpp2cadeletedmiceSPECIALIZATION：GeneticsPhDCANDIDATE：WeiqianCHENSUPERVISOR：XiangGAO，ProfessorAbstractProteinphosphatase2A(PP2A)isthemostabundantphosphatasespecificforserineandthreoninephosphorylationItiscomposedof

6、acommonheteromericcoreenzyme，thatiscomposedofacatalyticsubunit(C)andascaffoldingsubunit(A)，whichassociateswithavarietyofregulatorysubunits(B)Thecatalyticsubunit(PP2Ac)hastwoisoforms：PP2Acoc_(encodedbythePpp2cagene)andPP2

7、Acl3(encodedbythePpp2cbgene)PP2AcocisconsideredasthemainfunctionalPP2Acisoform，becauseofits10foldabundancethanPP2Acl3inmostmousetissuesDephosphoryIationofsubstratestargetedbyPP2Amediatesalargevarietyofbiologicalprocesses

8、IsuchasproliferationIsurvival|differentiationlmigrationandadhesionThevastarchiveofchemicalinhibitors，animalmodelsandrelatedstudiesdemonstratethatPP2Aphosphataseactivityisimportantintumorigenesis，nervedegeneration，hematop

9、oiesis，myocardiumcontractilemaintenance，andenergybalanceHoweverpreciseknowledgeaboutitsfunctionindevelopmentalbiologyespeciallytheestablishmentofthevascularandbloodsystem，needstobeanalyzedindetailInthisstudywedeletedthef

10、loxedPpp2calocusinendothelialandhematopoieticprogenitorcellswithaTie2CretransgeneBriefly40％Tie2Cre／Ppp2cdI／FembryosdiedbetweenEIO5andE145，whereas116％mutantswerebornnormallyUnexpectedlyPP2Ac僅一deficientembryosdidnotmanifes

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