組培微環(huán)境對(duì)草莓苗生根階段及移栽馴化過(guò)程中生長(zhǎng)和生理特性的影響.pdf

1、摘要本研究以未生根的草莓組培苗(Vg高1102cm，34片葉，豐香品種，由浙江省農(nóng)科院園藝所提供)為試驗(yàn)材料，通過(guò)控制組培生根階段的微環(huán)境，研究培養(yǎng)基中蔗糖添加量，培養(yǎng)時(shí)的光照強(qiáng)度和C02濃度對(duì)組培苗生長(zhǎng)和生理特性的影響，探討使組培苗趨于光合自養(yǎng)的組培環(huán)境是否比傳統(tǒng)的兼養(yǎng)環(huán)境更利于草莓組培苗的生長(zhǎng)：同時(shí)也研究了組培微環(huán)境的改變?cè)诮M培苗移栽馴化過(guò)程中的持續(xù)性影響，以期為組培快繁體系的進(jìn)一步完善提供理論依據(jù)＼『主要研究結(jié)果如下：、1生根階段

2、組培微環(huán)境對(duì)草莓組培苗生長(zhǎng)及光合的影響：未生根的草莓苗在3％(s)蔗糖或無(wú)糖培養(yǎng)基(NS)中誘導(dǎo)生根，同時(shí)分別給予60101』molm2S1(LL)和150109molra。2s1(HL)的兩種光照及35020虬L1(Lc)和70020gLL1(HC)兩種co。濃度。生根培養(yǎng)處理20d后，對(duì)草莓組培苗生長(zhǎng)形態(tài)指標(biāo)的測(cè)定結(jié)果表明，3％蔗糖有利于植株生物量的形成，提高光照和cO：濃度進(jìn)一步促進(jìn)試管苗的生長(zhǎng)。草莓組培苗的整體生長(zhǎng)勢(shì)以處理8(H

3、LSHC)和處理7(HLNSHC)較好處理6(HLSLC)和處理4(LLSHE)的次之。較高光照降低了葉綠素含量，而類胡蘿b素的相對(duì)含量有所提高。3％蔗糖和co：)JH富均提高了植株葉片的葉綠素含量。對(duì)組培苗生根培養(yǎng)結(jié)束時(shí)凈光合速率及葉綠素?zé)晒鈪?shù)(Fv／FmOPSII，qv)的測(cè)定表明，蔗糖的提供在未增加C0。的培養(yǎng)條件中對(duì)植株的光合有輕微的負(fù)影響。兩種光照條件下，cO：加富均顯著提高了植株的光合能力，尤其在較高光強(qiáng)下培養(yǎng)的植株。2生

4、根階段組培微環(huán)境對(duì)草莓組培苗移栽馴化過(guò)程中的影響：不同生根環(huán)境(處理同1)中生長(zhǎng)的草莓組培苗培養(yǎng)20天后，移栽至溫室內(nèi)馴化。不同組培生根環(huán)境對(duì)植株生長(zhǎng)形態(tài)的影響在移栽初期仍有一定地持續(xù)，但隨馴化時(shí)間的延長(zhǎng)，處理間植株生長(zhǎng)形態(tài)上的差異逐漸減小。移栽15天時(shí)，各處理間植株葉片的葉綠素含量和葉綠素／類胡蘿卜素的差異已基本消失。移栽初期，生根時(shí)于兩種光強(qiáng)下無(wú)糖培養(yǎng)的植株光合速率均有一輕微的下降，然后再上升。這與蔗糖處理的植株表現(xiàn)不同。，移栽馴化

5、后期各處理問(wèn)差異不明顯。與生根培養(yǎng)結(jié)束時(shí)相比，馴化末期各處理草莓植株的凈光合速率都有顯著提高。Fv／Fm在移栽過(guò)程中表現(xiàn)為先降后升，qbPSII和qP總體呈上升趨勢(shì)，說(shuō)明草莓苗光合機(jī)構(gòu)進(jìn)一步發(fā)展，光合能力得Effectsofmicroenvironmentonthegrowthandphysiologicalcharacteristicsofstrawberryduringrooting／nvitroandaccfimatization

6、exvitroYaohongZhou(Depar廿nentofHorticultureZhejiangUniversityHllajiacmCampus，Hangzhou310029)AbstractMicropropagatedstrawberry(FragariaananassaDuschsnecvFengxiang)wasusedtostudytheirgrowthandphysiologicalresponsestosucros

7、econcertrationaddedintheMSmedium，PPFDandC02concertrationduringrootinginvitroandthepersistenteffectsduringacclimatizationexvitrointheexperimentsTheresultsareasfollows：1Effectsofmicroenvironmentfactors／nvitroonthegrowthand

8、photosynthesisofstrawberryplantletsduringrootingstage：NodalcuttingswereculturedonsolidMurashige—Skoogmediumwitll3％sucroseorwithoutsucroseandinlow(60109molm2sI)orhigh(15010_tmolm。2s1)photosyntheticphotonfluxdensities，andn

9、ormal(350士50uLLJ)orelevated(7001001tLU1)C02concentrationfor20daysTheresultsindicatedthat3％sucrosewasbeneficialtobiomassformationofplantlets，higherPPFDandelevatedC02concentrationwerebothofbenefittothegrowthofplanfletsButh

10、i【gherPPFDreducedthechlorophyllcontent，andaslightnegativeeffectonphotosynthesiscouldbefoundinthecultureconditionsof3％SurOSewithoutelevatedC02AndelevatedC02washelpfulforthedevelopmentofphotosyntheticcapacity，especiallyund

11、erthehigherPPFD2Effectsofmicroenvironmentfactors／nvitroonthedevelopmentofstrawberryplantletsduringacclimatizationexvitro：After20daysofrootingculture(thetreatmentsweresameas1)，planfletsweretransplantedtothegreenhouseforam

12、onthperiodofacclimatizationAtthefirstdays，thedistinctiveeffectsofthedifferentinvitrocultureconditionsongrowthweremaintained，andalongwiththeacclimatizationthedifferenceingrowthamongthetreatmentsweredecreasedAtday15afertra

13、nsplantthedifferencesinchloro!c)hyllcontentandtheratioofchlorophylltocarotenoidscontentdisappearedAndaftertransplantthereWasallslightdeclineinnetphotosynthesisrateattheplanfletsculturedonmediumwithoutsucrose，thenascended