垃圾滲濾液污染地下水中硫酸鹽還原菌種群結構研究.pdf

1、上海交通大學碩士學位論文垃圾滲濾液污染地下水中硫酸鹽還原菌種群結構研究姓名：張雙月申請學位級別：碩士專業(yè)：環(huán)境科學指導教師：楊虹20060110上海交通大學碩士學位論文IIDiversityacterizationofSulfateReducingBacteriainGroundwaterpollutedbyLfillLeachateSeawaterABSTRACTSamplesinthisstudywerecollectedfromS

2、hanghaiLaoganglfillwhichgroundwatersystemwassubjecttothecombinedinfluencebytheleachateseawater.IndertolookfcluesabouttherelationshipbetweenSulfateReducingBacteria(SRB)communitystructurepullutionlevelthisstudyinvestigated

3、thediversityofSRBbyexplingthefunctionalgenedissimilatysulfitereductase(DSR).ConserveddsrABprimersinpolymerasechainreaction(PCR)generatedfulllengthdsrABamplicationsfcloningDNAsequenceanalysis.Becauseofthenutritionalversat

4、ilitygenerallycompletelyoxidizingSRBDesulfobacteraceaedominateSampleGSampleI(40.5%49.0%)butthehalophilicacterizationofthephylogenicgenerawasobviousinSampleI40.6%clonesbycontrastwith31.0%inSampleG.Thedifferenceofdominatin

5、gSRBbetweenLaogangotherlfillsathomeabroadisduetothespecialinfluencebytheleachateseawater.InSampleIthesecondarywasSyntrophobacteraceae(30.9%)anotherfamilyofgenerallycompletelyoxidizingSRB.ContrastivelyinsampleGthatwasDesu

6、lfobulbaceae(29.8%)whichwasgenerallyincompletelyoxidizingSRBseveraldsrABsequences(5.9%)wererelatedtohomologuesfromGrampositiveDesulfotomaculumspecies.TheanalysisindicatedthattheleachateseawateraffectthethediversityofSRBg