簡介：博士研究生田德斌指導教師袁世山研究員申請學位類別農(nóng)學博士專業(yè)預防獸醫(yī)學研究方向獸醫(yī)微生物及其分子生物學培養(yǎng)單位上海獸醫(yī)研究所研究生院提交日期2011年6月中國農(nóng)業(yè)科學院博士學位論文評閱人、答辯委員會簽名表論文題目嵌合動脈炎病毒的構建及其病毒學特性獸醫(yī)微生物論文作者田德斌專業(yè)預防獸醫(yī)學研究方向及其分子生物學指導教師袁世山研究員培養(yǎng)單位研究所、中心凈獸曼毋窘所、L’一‘R姓名職稱單位專業(yè)＼‘乏多簽名≮‘7J，R，L。，、汊芝蘭夕盲評|||評閱盲評|||L人盲評||||放知口辯主華修國教授上海交通大學預防獸醫(yī)學席鐘江教授復旦大學微生物學棚L孫建和教授上海交通大學預防獸醫(yī)學奶∥答馬志永研究員上海獸醫(yī)研究所預防獸醫(yī)學五H辯丁鏟研究員上海獸醫(yī)研究所預防獸醫(yī)學礬委于圣青研究員上海獸醫(yī)研究所預防獸醫(yī)學彳噸弼貝周金林研究員上海獸醫(yī)研究所預防獸醫(yī)學融鋤會議記錄秘書顧惠明論文答辯時間地點2011年6月8日，上海獸醫(yī)研究所A309室

簡介：中國農(nóng)業(yè)大學博士學位論文甜菜黑色焦枯病毒和煙草壞死病毒的比較病毒學研究姓名席德慧申請學位級別博士專業(yè)生物化學與分子生物學指導教師于嘉林李大偉20040601中國教監(jiān)大學博士學位論文ABSTRACTABSTRACTLHAVESTUDIEDTHEBEETBLACKSCORCHVIRUS，XINJIANGBBSVXANDNINGXIABBSVNISOLATESANDHAVEFOUNDPATHOGENICITYDIFFERENCESBEDNCONTHESETWOSTRAINSTHECOMPLETECDNACLONEOFBBSVXWASOBTAINEDBYRTPCROFTHEXINJTANGRNACOMPARISONSBETWEENBBSVXANDBBSVNREVEALEDANUCLEOTIDESEQUENCEIDANTITYOF98，9％，ANDTHEGENOMICSTRUTTUREWASALMOSTUNIFO巾1HOWEVER，THREEADDITIONAINUCLENTIDESWEREINTERSPERSEDBETWEENNUCLEOTIDES889AND975WITHINORF2INBBSVXTHESETHREENUCLEOTIDEDIFEE佗NCESRESULTEDINAFRAMESHIFFDIFFEREOCEINCOMPARISONSWITHTHEBBSVNSTRAINTHATAFFECTEDAMINOAEIDS28510312OFTHEP82PROTEINWHICHMAYBETHEREPLICASEASSOCIATEDPROTEINTHEAMINOACIDSEQUENCEIDENTITYOFTHEP82PROTEINSOFBBSVXANDBBSVNWAS954％IALSOCONSTRUCTEDANINFECTIOUSCLONEOFBBSVXBYPLACINGTHECD_NAUNDERTHECONTROLOFTHE啊PROMOTERBASEDONTHEINEECTIOUSCDNASOFBBSVNANDBBSVXIEXCHANGEDPARTIALFRAGMENTSOFTHEP82GENEAFTERINOCULATINGCHENOPODIUMAMARANTICOLORANDVIGNASINENSISWITHINVITROTRANSCRIPTS，MTRANTSRESULTINGFROMTHEP92FRAGMENTEXCHANGESANDWILDTYPEVIRUSWEREOBSERVEDFORPHENOTYPICCHANGES，BUTTHESYMPTOMSANDHOSTRANGEWERENOTALTEREDTHUS，THEREAPPEARST0BENODIRECTREL撕ONSHIPBETWEENTHEVARIATIONOBSERVEDINP82ANDTHEPATHOGENICITYOFTHETWOBBSVISOLATESANINFECTIOUSELONEOFTHESATRNAOFBBSVXWASALSOCONSTRUCTED塒PLACINGTHECD_NAUNDERTHECONTROJOFTHE35SPROMOTERTHISPLASMIDCOULDINFECTCHENOPODIUMAMARANTO如RWITHTHEHELPOFINVITROTRANSCRIPTSOFBBSVXSIXDIF托RENTDELETIONANDINSERTIONMUTANTSOFBBSVXWERECONSTRUCTEDANDTHESEWEREINOCALATEDTOCHENOPODIWNAMARANTICOLOR11地PRELIMINARY船ULTSSHOWTHATTHESATRNACOULDENHANCESVMPTOMOFBBSVXANDITSJNSERTIONMUTANTSONCHENOPODIWNAMARAMICOLORBUTTHEPATHOGENICITYOFTHECPDELETIONMUTANTS蟠REDUCEDINORDORTODETERMINETHERELATIONSHIPBETWEENBBSVANDOTHERNECROVIRUSMEMBERS，INCLUDINGTHETYPESPECIESTOBACCONECROSISV0USTNV，IALSOSTUDIEDTHEBIOLOGICAL，PHYSICAL，CHEMICALANDSEROLOGICALPROPERTIESOFTHECHINASOYBEANISOLATEOFTNVR簿DATAP讒SENTEDBELOWINDICATE也缸THISISOLATEISANEWSTRAINOFTNVAWHICHIHAVED髓IGNATEDNⅣA。1K1NV小ISOLATEHASAHI曲STABILITYINVITROANDALSOHASABROADHOSTRANGEINCLUDING29SPECIESIN8FAMILIESTNVA。GENERALLYINDUEES10COLLESIONSINMOSTHO啦BUTSOYBEANANDⅣBENTHARAIANAALESYSTEMICHOSTSIPURIFIEDTHEVIRUSANDTHENOBTAINEDAHIGHTITER_NQVA。ANTISERUMTHERESULTSOFSEROLOGICALSTUDIESSHOWEDTHATTNVA。ISCLOSELYRELATEDTOTHETNVWILLOWISOLATEBUTFAILSTEACTWITH“INVDANDBBSVBASEDOBTNVSEQUENCESREPORTEDPREVIOUSLY，LDESIGNEDDEGENERATEPRIMERSANDOBTAINEDSUBCLONESREPRESENTINGPORTIONSOFTHETNVA。GENOMETHECOMPLETESEQUENCEOFTHEISOLATEWASDETERMINEDBY5“RACEAND3’RACETHEDATAREVEALTHATTHEVIRUSENCODESFIVEORFSINA3682NTGENOME，ANDTHATTHENUELEOTIDESEQUENCEIDENTITYISRESPECTIVELY864％437％AND443％BETWEENTHE1NVA。ISOLATEANDTHOSEOFTNVA，TNVDANDTNVD“WHOSESEQUENCESHAVEBEENREPORTEDPREVIOUSLYTHUSACCORDINGTOTHEBIOLOGICALPROPEAIESMADNUCTEOTIDESEQUENCESOFTHESESTRAINSIPOSTULATETHATTHESOYBEANISOLATEISANEWSTRAINOFTNVATOPRODUCEINFECTIOUSCDNACLONESTNVA。CDNAWASPLACEDUNDERTHECENTRELOFTHET7ANDTHE35SPROMOTERS，ANDDEIETIONMUTANTSINOR1，ERR3，ORF4ANDORF5WEREGENERATEDTHROUI曲ANALYZINGTHESYMPTOMSANDGENOMERNAREPLICATION，ICONCLUDETHATTHEORFIGENEISTHEREPLICASEASSOCIATEDGEREANDTHATORF3ANDORF4ARECELLTOEELLMOVEMENTGENESTHEPROTEINENCODEDBYORF5AFFECLSLONGDISTANCEVIRUSMOVEMENTWHICHRESULTSINATWODAYDELAYOFLESIONAPPEARANCEINCHENOPODIUMAMARANTICOLORKEYWORDSBEETBLACKSCORCHVIRUSPATHOGENICITYDIFFERENCE，TOBACCONECROSISVIRUBIOLOGICALPROPERTIES，INFECTIOUSEDNACLONE11