被子植物基部類群三白草和細(xì)辛開(kāi)花相關(guān)基因的克隆，功能與進(jìn)化的研究.pdf_第1頁(yè)
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1、中國(guó)農(nóng)業(yè)大學(xué)博士學(xué)位論文被子植物基部類群三白草和細(xì)辛開(kāi)花相關(guān)基因的克隆，功能與進(jìn)化的研究姓名：趙銀河申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：遺傳學(xué)指導(dǎo)教師：王國(guó)英李德銖20061101AbstractFloweristhemostimportantorganinthegrowthanddevelopmentofplantsFromanevolutionarystandpointithaslongbeenapredominantsoureeofchara

2、cters衙∞酉0spcmtaxonomyBasalan舀偽p啪nsa他veryimportantfor咖dyingtheorigindiversificationandevolutionofangiospermsSaunvuschinensis(Saururaeeae)andAsarumcaudigeman(Aristotochiaeeae)areanimportantspeciesofpaleoberbinrelationtound

3、erstandtheoriginandevolutionofangiospermflowersduetoitsbasalpositionintheangiospermsAsthememberofpiperalestheflowerof&ehinemisisverysimplewhichbearhaetswithoutperiantlnandevenwthoutpetalprmordiaduringfloralontoganesisWhi

4、leAsarumcaudigerum(Aristoloehiaceae)whichhasonlyol埒whorlofperianthanditsfloralformisverysimilartothebasalmostangiospermAmborellaThesubtractedfloraleDNAlibrarieswereconstructedfromfloralbudsandinflorescenceatdifferemtstag

5、esusingsuppressionsubtractivehybridization(SSH)TheI斌temofgeneexpressprofileoffloralrelatedgenesisolatedfromAcaudigerumwitharrayandfurtherconfirmedbyrealtimePCRandobservedwithScanningelectronmicroscope(SEM)aswellBasedonth

6、eESTsequeaecofDV038650，twogenespeeificprimersweredesignedandusedforrapidamplificationofeDNAands(RACE)procedureTofurtheridentifythefunctionofputativeMADSbextranscriptionfactorsphylogenetietreeswerereconstructedinordertoin

7、ferevolutionaryrelationshipswithintheMADSboxgenefamilyintheforwardsubtractedfloralcDNAlibraryof彳caudigerum2320cloneswererandomlysequencedfromwhich612uniqueexpressedsequencetagsmSTs)wereobtainedand154floralrelatedgenesupr

8、egulatedwerefoundusingarrayintheupmostleavesperianthstamensandcarpetrespectivelyAfulllengthputativeMADSboxgenewasobtainedandclonedusingRACEWhileintheforwardsubtractedfloralcDNAlibraryof&chinensis548clonesweierandomlysequ

9、encedandatotalof432uniqueexpressedsequencetags(ESTs)wereobtainedThe4PETALd3(AP3)likeofBclassgeueisolatedfromzeaudigerumwereupregulatedintheperianthstamalsandcarpelfromthepatternofgeneexpressprofileoffloralrelatedgenes，an

10、dfurtherconfirmedbyrealtimePCRandScauningelectronmicroscope(SEM)ThisimpliedthatagradualshiftinexpressionofBelassgenesmaybeaglos$thefloralparts，andsuggestedthattheABCmodelmaynothavebecomefixedintheearlystageoftheangiosper

11、mevolutionandmaybemodifiedorsupplementedinthebasalangiospermPhylogeueticanalysisindicatedthattheMADSboxtranscriptionfactorsisolatedfrom一caudigerum。andthreeoutof12MADSboxtranscriptionfactorsfrom&chinensisweremembersofAPET

12、4LA3(AP3》subfamily，whichformedaclusterwithMADSboxgenesisolatedfromAmborellathebasal—mostangiosperm。andthosefromthegymnospermsandwassisterrelationshipbetween&chinensisandAcaudigerumOurresultssuggestthattheoriginofSchinens

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