18836.西南印度洋深海熱液羽流水體細(xì)菌多樣性研究

1、中國科學(xué)院研究生院碩士學(xué)位論文西南印度洋深海熱液羽流水體細(xì)菌多樣性研究姓名：任菲申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：戴欣201205AbstractDeepseahydrothermalplumeisanimportantpartofglobalmidoceanridgehotspringfieldandplaysanessentialroleinthedeepseaheatandmaterialcirculation，leadi

2、ngtoasignificantimpactonglobaloceancirculationandoceanchemistryDuetemotelocation，SoulhwestIndianRidge(swm)hasbeenhardlystudied，SOweknowlittleaboutbacteriadiversityintheenvironmentThisthesisfocusedonthebacterialdiversityi

3、nadeepseahydrothermalplumeseawaterinSWIR，notonlytoincreaseourunderstandingoftheimpactofthemicroorganismsontheoceanecosystem，butalsotoobtainmicrobialresourcesinthisspecialenvironmentThedeepseahydrothermalplumeseawaterinth

4、esouthwestIndianOceanwasconcentratedinsituby1000folds，enrichmentcultureswereestablishedwiththeconcentratedsample，andisolateswerepurifiedBacterial16SrRNAgenelibrarieswereconstructedfromboththeconcentratedseawatersampleand

5、fromtheenrichmentcultureandanalyzedThe16SrRNAgenesfromtheisolatedstrmnswerealsoanalyzedAtotalof10516SrRNAgeneswereobtained，inwhich50werefromtheconcentratedplumeseawater，40fi’omtheenrichmentculture，and14fromtheisolatedstr

6、ainsThesesequencesareaffiliatedwithTproteobacteria(74)，0【proteobacteria(15)，13Proteobacteria(5)，Bacteroidetes(4)，F(xiàn)irmicutes(2)，Planctomycetes(2)，Verrucomicrobia(2)andActinobacteria(1)，andfallinto30differentoperationaltax

7、onomicunitsl(OTUs)Twentysevensequencessharelessthan97％identity麗ththebest—matchedsequencesinthepublicdatabase，withthelowestbeing86％Inthoseisolates，the16SrRNAgeneofstrain8047Tshowedleastidentitywithknownones(93％)Thecharact

8、eristicsofstrain8047TweredetailedbyusingapolyphasicclassificationapproachandphylogeneticanalysisTheresultsshowedthatstrain8047TshouldbelongtoanewgenusinAurantimonadaceae，Rhizobiales，forwhichthenameHuangiamanganoxydanswas