載BCV-N蛋白的殼聚糖微球的制備及其免疫效果的研究.pdf

1、黑龍江大學碩士學位論文載BCVN蛋白的殼聚糖微球的制備及其免疫效果的研究姓名：車小瓊申請學位級別：碩士專業(yè)：微生物學指導教師：孫慶申20090508黑龍江大學碩士學位論文AbstractChitosanwhichisapositivepolysaccharidecomposedof2amino一2一deoxypDglucancombinedvvithglycosidiclinkages，ispreparedbyalkalinedeace

2、ty’lationofchitinAsakindofbiomedicalmaterial，chitosanhasmanypropertiessuchasgoodphysico—chemicalcharacteristics，biocompatibilityandbiodegradabilitywhichmakeitusefulinpharmaceuticalformulations，andtissueengineeringfieldsu

3、chasartificialvessel，surgicalsuture，andtumortherapyBovineCoronavirusVtms(BCV)isamemberbelongingtoCoronavirusfamilyandthelargestRNAvirusknowninthebiosphereThisviruscancausediarrheaandslightlyrespiratorytractinfectionsympt

4、omsInadditiontobovine，BCVCanalsoinfecthuman，pig，horse，sheepandmanyotheranimalsThepurposeofthispaperistopreparechitosanmicrospherecontainingBCVandstudyitssustainedreleaseprofileinvivoBlankchitosanmocrosphereswerepreparedb

5、yemulsificationandcross—linkingmethodusing百utaraldehydeascrosslinkersThebovineserumalbumin(asA)wasincubatedorinoculatedintothechitosanmicrosphereasmodelproteinTheresultshowedthatthemicrosphereshavegoodmorphologyandappear

6、anceAloadingefficiencyupto1667士334％，32824638％(w／w)andaloadingcapacityupto21934430％、50654609％(w／w)wereobtainedbytheincubationandinoculationmethods，respectivelyInvitreleasestudiesshowedthatmorethan70％oftheproteinpreparedby

7、incubationmethodWasreleasedduring120hinPBS(pH72)at37“C；andonly20％wasreleasedbyinnoculationmethodat48htheseresultsindicatedgoodsustained—releaseprofileofthechitosanmicrosphereThetoxicityofthechitosanmicrospheresWastestedb

8、yinvitrocytotoxicityassaysusingBHKcellswhichshowedlowtoxiceffectsoftheglutaraldehydeerosslinkedmicropheresThe切vivoimmuneeffectofBCV斟loadedchitosanmicrospheresbyinjectioninratsWasstudiedbyELISAassaysTheresultshowedmatat12