白前生物堿的制備及其對斜紋夜蛾的拒食作用研究.pdf

1、Y1107951哼鏑碧薏夭等碩士學(xué)位論文白前生物堿的制備及其對斜紋夜蛾的拒食作用研究ThePreprationofAlkaloidsfromCy月anchummotzgolicumandTheStud)ofItsAntiltedingEffectsoR印odopteralitura指導(dǎo)教師：五亞鱧』扯垃控————一申請學(xué)位類別：韭堂耍土——————一專業(yè)領(lǐng)域名蔣：盤些且蟲皇蠱魚盟擅一研究方向：益蟲生物盟揎————一所在學(xué)院：盤堂皇生物拉

2、盎堂院2007年6月AbstractInthisstudythebioactivecomponentsagainstSpodopteraliturawereextractedfromCynanchummongolicura(Maxim)ThebioactivitycomponentswhichhadstrongantifeedantactivityandshouldbealkaloidwereacquiredTheactionmodes

3、ofthesecomponentsagainstSpodopteraliturawerealsoinvestigatedinthelaboratoryTheresultswereasfollows：1、TheairdriedpowderedCynanchummongolicumwasexhaustivelyextractedwithindustrialalcoholandgummyresidueWaSobtainedTheresidue

4、WaSacidifiedwithHCl，andbasifiedwithNaOH，thenextractedwithseveralkindsoforganicsolventtoobtainCynanchummongolicumalkaloidandpurificatoryCynanchummongolicumalkaloidTheextractingrateofCynanchummongolicumalkaloidandpurificat

5、oryCynanchummongolicumalkaloidwereO164％and00916％respectivelyCynanchummongolicumisanalkaloidalplantAlkaloiddeterminantreagentshowedthatCynanchummongolicummainlyhadsmallandmiddlepolarityalkaloid，thealkaloidextractedbychlor

6、oformWaSthedominatingalkaloidThethinlayerchromatographyshowedthatCynanchummongolicumhadmorethan3kindsofalkaloidTheantifeedantactivityofCynanchummongolicumalkaloidagainstPlutellaxylostellaenhanced，astheconcentrationofCyna

7、nchummongolicumalkaloidincreased2、TheantifeedantactivitiesofextractsagainstSpodopteralituralarvaeweretestedTheresultsareasfollows：theextractshadverystrongactivitiesagainstthe3rdinstarand5rdinstarlarvaewhentheconcentratio

8、nreachedO5mgml～anddecreasedwithaconcentrationof001mg。ml1ThenonselectiveAFCsoforthe3rdand5rdinstarlarvaewere00139mg‘ml～and00164mg‘ml“respectivelyaftertreatedfor24hourstheselectiveAFCs0wereO0099mg’ml。and00149mg’m11respecti

9、vely3、ReversephasesemipreparativeHPLCWaSusedtoseparatealkaloidsfromCynanchummongolicumAHPLCmethodwasestablishedtoanalyzetheproductsTheresultsareasfollows：therewasonemajorcomponentinthecentrifugalalkaloidwhichalsohadstron

10、gactivitiesagainstthespodopteralituralarvae；andmorethantenmutualpeakswereconfirmedfromCynanchummongolicumalkaloidsThereare6majorcomponentsandtherentiontimeare3836rain16276rain，19488min，20664min，2303Imin，39856rainrespecti