小麥溫敏雄性不育系BS366不育機理探討.pdf

1、分類號:華中農(nóng)業(yè)大學博士學位論文小麥溫敏雄性不育系BS366不育機理探討StudiesonthemechanismofthermosensitivegenicmalesterilityofwheatlineBS366研究生:唐忠輝指導教師:指導小組:鄭用璉教授鄭用璉教授趙昌平研究員張祖新教授嚴建兵教授專業(yè):生物化學與分子生物學得學位名稱:理學博士研究方向:植物雄性不育提交論文時間:2011年4月二。一一年六月小麥溫敏雄性不育系BS366

2、不育機理探討2.10.5miRNA以及ta一siRNA靶基因預測..................................................……262.10.6降解組數(shù)據(jù)分析.................................................................................……272.11qPCR(quantitativereal一timePCR)驗證

3、...................................................……272.12靶基因剪切位點驗證..................................................................................……273結果...............................................................

4、...........................................................……293.1溫敏雄性不育系BS366雄蕊敗育細胞學研究...........................................……293.2低溫脅迫下BS366雄蕊組織基因表達譜...................................................……犯3.2.1篩選溫敏雄

5、性不育系BS366普通低溫脅迫響應基因.....................……373.2.2溫敏雄性不育系BS366雄蕊組織基因表達譜時間序列分析.........……403.2.3細胞骨架相關基因受低溫脅迫影響..................................................……423.3SmalRNA和降解組Solexa測序...................................

6、.............................……463.3.1溫敏雄性不育小麥BS366miRNA鑒定...........................................……483.3.1.1miRBase中己知而RNA鑒定...................................................……483.3.1.2新m1RNAS預測.....................

7、...................................................……493.3.2溫敏雄性不育系BS366TAS基因識別..............................................……563.3.3降解組數(shù)據(jù)分析...............................................................................

8、...……573.3.3.1而RNA靶基因降解組結果.……，...............................................……573.3.3.2ta一siRNA革巴基因降解組結果.....................................................……593.3.3.3新而RNA的靶基因降解組結果.....................................

9、........……593.4而RNAs、tasiRNA一ARf表達量qPCR驗證...............................................……613.4.1參與生長素信號傳導途徑調(diào)控的而RNA、tasiRNA一ARF表達量分析........................................................................................

10、................................……613.4.2而R166家族的而RNAS表達量驗證.................................................……623.4.3其它而RNAS表達量驗證..................................................................……623.5靶基因表達量驗證以及降解位點證實..

11、......................................................……623.5.1而R166家族成員靶基因的表達量分析.............................................……643.5.2ath一而R156a、osa而R169e和osa一而R319a靶基因的表達量分析……643.5.3靶基因ARF的表達量分析........................

12、........................................……643.5.4靶基因ARF降解位點5，RIJMRACE證實.....................................……693.6而RNA原位雜交分析...................................................................................……703.7內(nèi)源IAA

13、定量檢測.......................................................................................……724討論..........................................................................................................................

14、……754.1溫敏雄性不育系BS366絨氈層發(fā)育不受低溫脅迫影響...........................……754.2低溫脅迫導致二分體形成時細胞質分裂異常............................................……764.3低溫脅迫下溫敏雄性不育系BS366穗部組織miRNA表達....................……784.4而RNA、tasiRNA一AR王對轉錄因子ARF調(diào)控作用.

15、..................................……794.5生長素信號傳導途徑與細胞骨架................................................................……81參考文獻.............................................................................................

16、.........................……83致謝..........................................................................................................................……100附錄............................................................

17、..............................................................……101附錄1.........................................................................................................................……101附錄2.....................

18、....................................................................................................……104附錄3................................................................................................................