壓電體聲波生物酶解反應(yīng)與金屬表面過程傳感研究與應(yīng)用.pdf

1、湖南大學(xué)博士學(xué)位論文壓電體聲波生物酶解反應(yīng)與金屬表面過程傳感研究與應(yīng)用姓名：何德良申請學(xué)位級別：博士專業(yè)：分析化學(xué)指導(dǎo)教師：姚守拙2000.9.1塑塑查蘭坌塑些堂妻、業(yè)壁主蘭壁絲苧!墾皇竺蘭絲皇塑鬯竺墾鏖皇壘墨墨耍絲堡堡壁塑塑蘭壁里!塑堡墾2ABSTRACTPiezoelectricsensorhasfounditsextensiveapplicationsinvariousfieldsincludinganalyticalchemis

2、try，electrochemistry，surfacescience，lifescienceandenvironmentalmonitoringbecauseofitsoutstandingadvantagessuchasbroadsensingspectrum，highsensitivity，simpleconstructionandlowcost，etcTheresearchmethodsforpiezoelectricquart

3、zcrystal(PQC)sensorsincludeactivemethodandpassivemethodIntheactivemethod，onlyasimpleapparatus，suchasfrequencycounter，isrequiredandconvenientoperationisneededByusingthepassivemethod，muchmoreinformationincludingimpedance，a

4、dmittance，phaseangleandfrequencyofPQCCanbeobtained，theequivalentcircuitparametersofPQCresonatoracquiredfromtheconductanceandsusceptancedatacallbeusedtoinvestigatethechangesinmassloadingattheelectrodesurface，viscoelastici

5、tyattheinterfaceviscosityanddensityandconductivityofthesolutionThebulkacousticwavesensingtechniquecanbeappliedtomonitorcontinuouslysomeprocessessuchasenzymereactionandmetalsurfacefinishingandtodeterminethekineticparamete

6、rsandthecharacteristicsofsurfacereactionThemainworksaresummarizedasfollows：1Theenzymatichydrolysisofsodiumcarboxymethylcellulose(CMC)bycellulasehasbeenstudiedbybulkacousticwave(BAW)cellulasesensingtechniqueThehydrolysisr

7、ateofCMCunderdifferentpHconditionsat30。Cwasdeterminedandusedtoestimatekineticparameters(theMichaelisconstantKmandthemaximumrateV)ofthereactionbyusingthelinearLineweaverBurkplotmethodTheactivationenergy(E。)oftheenzymaticr

8、eactionwasestimated2AnewmethodfortheimmobilizationofctamylasebyUV—curingcoatingonPQChasbeenproposedTheinitialhydrolysisratewasmeasuredatdifferentsolutionpHandsubstrateconcentrationsandusedtostudytheeffectoftheimmobilizat

9、ionprocessbyUV—curingcoatingond—amylaseactivityKineticparametersoftheenzymatichydrolysisofstarchbytheimmobilizedⅡ一amylasewereestimatedalSO、3Thecorrosioninhibitionbehaviorofmercaptobenzothiazole(MBT)oncopperin01molL—H2SO。