Ferulic Acid Inhibits UVA-induced Photoaging in Human Skin Fibroblasts through Telomere Pathway.pdf

1、In this study, we aimed to observe the effects of UVA irradiation and ferulic acid intervention in human skin fibroblasts and investigate whether ferulic acid could protect cultured human skin fibroblasts from UVA irradi

2、ation induced photoaging through telomere pathway, in order to explore the possible mechanisms ofphotoaging delay.Subconfluent fibroblasts were divided into normal control group, ferulic acid group,UVA irradiation group,

3、 and UVA plus ferulic acid group.Fibroblasts were shammed or irradiated with 10J/cm2 of UVA irradiation and treated with 200μg/mL of ferulic acid.In our laboratory setting, telomere length was measured by real-time quant

4、itative PCR, while telomerase activity and expression were measured by two different methodologies.For the purposes of quantifying telomerase activity and expression in the context of clinical research, the most common n

5、onradioactive techniques used include the highly sensitive photometric enzyme immunoassay, TRAP-ELISA assay, and real-time quantitative PCR analysis for expression of telomerase activity and mRNA levels of hTERT genes,re

6、spectively.These methods are largely sufficient for assessing overall telomerase function within a tissue of interest.The present findings potentially provides the basis for better understanding of the molecular mechanis

7、m of UVA-induced photoaging and the protective effect of ferulic acid against it through telomere signaling pathway.The intervention of ferulic acid could restore the telomere length compared with UVA group (p＜0.5), whil

8、e the expression of telomerase activity was undetectable and hTERT mRNA expression was decreased.These results have important implications for further research of ferulic acid role and mechanism in photoaging.The results

9、 indicated that the telomeres were shown to be adversely affected by UVA
　　irradiation and the damage caused was related to cell aging, confirmed that telomeres rather than telomerase are more involved in cellular sen

10、escence.and hence ferulic acid can significantly delay the shortening of telomere length of skin fibroblast either under physiological state or post-UVA irradiation, and postpone cellular senescence, but it is not throug