玉米秸稈發(fā)酵制乙醇的試驗研究.pdf

1、寧夏大學碩士學位論文玉米秸稈發(fā)酵制乙醇的試驗研究姓名：劉志良申請學位級別：碩士專業(yè)：分析化學指導教師：楊敏麗20100301AbstractThefuelethanolproducedbycornstalkfermentationnotonlyeasetheglobalenergycrisisbutalseducethegreenhouseeffectandenvironmentalpollutioncausedbyusingoffos

2、silenergyandstrawburningWiththeworseofenergycrisisandenvironmentalpollution，ithasbecomemoreandmoreurgenttoproducefuelethanolthroughfermentationofcornstalkInthispaper，thecornstalksfromNingxiafarmwasusedasrawmaterialforthe

3、productionofethanolbybiofermentation，thefactorsinfectedthesamplepretreatment，enzymaticsaccharificationofcellulose，andCOIUSCoffermentationwereexaminedandtheconditionswereoptimizedthroughorthogonalexperimentIntheprocessofs

4、amplepretreatment，COrnstalkswererespectivelypretreatedbyorganicsolvents，dilutedacid，dilutedalkalineandthecombinationofalkaliandacidTheresultsshowedthatthecombinationofalkaliandacidisbetterthanothersolventsforthepretreato

5、fcornstalks，whichgivethesaccharificationrateof6616％，andfurthermore，celluloseandhemicelluloseCanbeseparatedbythismethodwhichisbeneficialforthefollowfermentationofxyloseandglucoseundereachbestconditions，whichCanincreaseeth

6、anolproductionThepretreatedComstalkswassacch撕fiedbycellulase，andtheinfluencefactorsontheenzymatichydrolysis，includingtheconcentrationofsubstrate，saccharifiedtemperature，saccharifiedacidity，saccharifiedtimeandEnzymedosage

7、werestudiedusinghydrolysisrateasindexTheorthogonaltestresultsshowedthattheoptimalhydrolysisconditionswere：concentrationofsubstrate(1％)，Enzymedosage(2000u／m1)，pHvalue(49)，temperature(45。C)，andtime(48h)Undertheoptimalcondi

8、tionthesaccharificationrateisreach8624％TheimmobilizedenzymeismorestableintheactiveandCanbeusedformanytimes，illthispaperthecellulasewasimmobilizedonsilicagelswithglutaraldehydeascrosslinkingagentandwasusedtohydrolyzecorns

9、talkThefactorsinfectedtheactiveofimmobilizedenzymeandthefactorsinfectedtheenzymaticsaccharificationwerestudiedrespectivelyThroughorthogonaltests，theconditionsofimmobilizedcellulasewereoptimizedwhichwere：glutaraldehydel％，

10、pHvalue50，amountofenzymeimmobilizedonchitosanlOOm∥gsilicaTheenzymaticsaccharificationconditionswere：reactiontime48h，concentrationofsubstrate2％，temperature(50。C)，pHvalue(40)，Enzymedosage(06mg／g)Thefactorsinfectedthebiofer

11、mentationincludingacidity，fermenttime，thequantityofinoculation，temperaturewereoptimized，throughorthogonalexperimenttheparameterswereoptimizedwhichwere：pH55，time(66h)，10％inoculumsolution，temperature42“CUndertheoptimumcond