CD44及CD44v6在肺癌中的表達研究.pdf

1、中國協(xié)和醫(yī)科大學碩士學位論文CD44及CD44v6在肺癌中的表達研究姓名：付英杰申請學位級別：碩士專業(yè)：病原生物學指導教師：劉力20060501中國醫(yī)學科學院中國協(xié)和醫(yī)科大學碩十學位論義ExpressionAnalysisofCD44StandardForⅢandItsV6SplicedVariantinLungCarcinoⅢasAbStractCD44isafamilyoftrans—membra兀eglycoproteinthat

2、iswidelyexpressedinmanytissuesderivedfromepithelial，mesenchyⅡIal，hematopoieticand【nesodermaloriginsCD44proteinwasinitiallyidentifiedasaguideforlymphocytehoming，andthenwasfoundtoplayimportantr01esinⅡIanyphysi0109icalandpa

3、th0109icalprocessessuchashematopoiesis，immuneresponse(1ymphocyteactiVationandhoming)，development，woundhealing，inflammation，andtumormigrationInthisstudy，weex鋤inedtheexDressionofCD44sandCD44v6in30lungcarcinomastissues，3cel

4、llinesof1ungcarcinomas，and16normallungtissuesonDNA，RNA，andproteinlevels，andcorrelatedthefindingswithtumorstage，histologicaldifferentiationAsaresult。wedemonstratedthat：1CD44sexpressedinkindsoflungcancer，includingSSC，SCLCa

5、ndadenocarcinoma2TheexpressionofV6SplicedVariantinLungCarcinomasonRNAlevelappearsdiversity3TheexpressionofCD44v6inSCLCisvery10wTherewasasignificantdifferencebetweennormallungtissueandSCLCabOutmethylationofCD44promoter(p0

6、05)4OurresultsrevealedthereversedpatternofCD44geneexpressioninsqu硼ouscelllungcarcinomaversuslungadenocarcinomaInsquamouscelllungcarcinoma，thenumberofCD44spositivecasesdecreasesasthegradingincreases，whilein1ungadenocarcin

7、omaitreachestheoppositeConclusion：TheresultssuggestthatCD44sandCD44v6areexDressedwithdifferentdegreein1ungcarcinomasTheexpressionofCD44v6inSCLCisverv10wandtherewasasignificantdifferencebetweennormallungtissueandSCLCabout

8、methylationofCD44promoter，whichmaybeindicatedthatmethvlationeffectedtheexpressionofCD44andCD44v6CD44v6ismostcloselvassociatedwith1ungcancer，especial1ywithSSC，whichmaybeasanindexforhist0109icaldifferentiationof1ungcancerK