百合體細(xì)胞和雄配子體染色體加倍的研究.pdf

1、南京林業(yè)大學(xué)碩士學(xué)位論文百合體細(xì)胞和雄配子體染色體加倍的研究姓名：張靜申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：林木遺傳育種指導(dǎo)教師：施季森20070601StudyonpolyploidyinductionofLilybySomatictissueandpollenABSTRACTOrientalhybridsandLongiflorumhybridsareveryimportantinlilycutflowersontheworldmarketThe

2、yactasornamentalandcommercialfunctionwithhigheconomicalvalueSochoosingtwoofthem，LiliumsiberiaandLiliumLonginorumasmaterials，westudiedonthesomatictissueandpollenchromosomedoublingthroughtissuecultureandbudstreatmentInthis

3、way，wecanfoundthetechnicalandmaterialresourcesgroundforcreatingartifitiallilytriploidFoundingthetissueculturesystemisoneofthepreconditionsfortheinductionoflilysomatictissuepolyploidsTheresultoftissuecultureshows：themosta

4、ppropriatemediumforbulbscalegerminationofLsiberiaisMSSUCrOSe4％BA10mg／LNAA05mg／LmediumTheconcentrationandmixtureratioofhormonearetwooftheimportantfactorsinbudinducingTheconcentrationofsucroseisthemostimportantfactorinbulb

5、letsgrowth，andthemostappropriatemediumforrootinginductionandsoundseedlingofbulbletsisMSsucrose8％NAAO3mg／LKnowingthediploidchromosomenumberandkaryrotypeof己，siberiaisanotherpreconditionforitssomatictissuepolyploidsinductio

6、nThroughtheobservationofmitosiscourseofLsiberia，wegettheconclusionthatthediploidychromosomenumberof￡siberiais2n=2x=24，itskaryotypeformulais2n=2x=24=4m2t14st4sin(SAT)，belongingto3BtypeThen，Weusephysicalandchemicalmethodst

7、oinduceLsiberiasomatictissuepolyploids，andintheendwefind：theeffectofphysicaltreatmentisnotobviousbecausewejustgetsomephynotypevariationbulbletsbutnopolyploidyceilsaftertheevaluationofchromosomenumberPolyploidyinductionby

8、chemicalmethodsiscarriedOUtwithcolchicinebytowways，thatisculturedonthemediumwithcolchicineandmarinatinginthesolutionofcolchicineBythewayofculturedonthemediumwithcolchicine，Weonlyget2mixoploids，theircellssomearediploidsan

9、dothersaretetraploids，andwehavenotfindpuretetraploidsThebestconcentrationofcolchicineare002％and005％，andthebesttimeofitstreatmentis48hbymarinatingwayAfterthesetreatments，weget4tetrapliodsinall，theratesoftetraploidycellsar