樟子松針葉活性成分研究.pdf

1、⑧天畢大蓼目近代第所女學(xué)碩士學(xué)位論文一_㈣㈣Ⅲ■●l一學(xué)科專業(yè)：藥物僉圭丘作者姓名：塑萱指導(dǎo)教師：監(jiān)室皇塑撞合作導(dǎo)師：直童重塾攮工浩當(dāng)zⅡ巒生R12005年I2月ABSTRACTPinusSylvestris(PinusL)isakindoflargegreenarboryearoceouringmainlyinDongbeiProvinceChinaTheneedlesofPSylvestrishaveanti—inflammator

2、yoxidationresistingandantivirnseffectsItsmajorchemicalconstituents，includingditerpenes，naphthaflavonesandSOon，havebeenreportedPharmacologicalexperimentsindicatethatthePEandEtOAcextractsofthisplanthavesignificantanfitumor

3、andimmunosuppressiveactivitiesInordertosearch也ebioactiveconsitituentsfromnaturesource，webegantostudythechemicalconstituentsfromtheneedlesofPSylvestrisByrepeatedcolumnchromatographyincludingSilicagel，ToyopearlHW40Candprep

4、arativeHPLC，threenew(13)andtwentythreecompoundsf4—26)wereisolatedfromtheEtOHextractofPSylvestrisBymealKSof1HNMR，13C廿懈and2D0妊嗄Rspeeaathestructuresofnewcompoundswereelucidatedas15ethyl一18methylpinifolate(1)，15Acetoxy—labda

5、8(17)，13E—dien18一al(2)，18Hydroxylabd一8(17)，13Edien15acetate(3)Compound4(15Acetoxy—labd一8(17)一en18一oicacid)isanewnaturalproducLandseventeencompoundswereisolatedfromPSylvestrisforthefirsttimeasfollowed：18ct—Acetoxylabd8(17

6、)ca一15一oicacid(8)，3pHydroxy一8(17)，E一13labdadien15oicacid(10)，Dehydroabieticacid(11)，7ctHydroxydehydro—abieticacid(12)，7p—Hydroxydehydroabieticacid(13)，7ct，18dihydroxydehydro—abietanol(14)，pimarol(16)，pimadcacid(17)，isopi

7、marol(18)，isopimaricacid(19)，7oxo一13一epipimara8，15dien一18oicacid(20)，0【Cadinol(21)，TCadinol(22)，eudesm11en4etol(23)spathulenol(24)，oplopanone(25)and13daucosterol(26)OurpreviousstudyshowedthatthePEandEtOAcextractsrevealed

8、significantantitumoractivitiesandthefilrtherresearchforisolatedcompoundsw盱cscreenedbycytotoxityeffectswithHelaandA549cellsCompound12revealedsignificantinhibitoryeffectonHelaandA549，compounds7and13showedpositivelyinhibito

9、ryeffectonHelaandcompounds14and15revealedpositivelyinhibitoryeffectonA549InthelymphocytetransformationexperimentionthePEandEtOAcextractsrevealedthesignificantinhibitoryeffecttotheconcanavalin(ConA)controlgroupQ001，n=6)an