癌細胞和血液的特征熒光光譜研究.pdf

1、電子科技大學碩士學位論文癌細胞和血液的特征熒光光譜研究姓名：李靜申請學位級別：碩士專業(yè)：生物物理學指導教師：龐小峰20090501ABSTRACT硼1ce任ectivecanc盯identificationanddiagnosishavetakenonaconsiderableimportanceinsavingcAnc貧patients’lives，especiallyintheearlystageAmongthevari懈diagn

2、osistechniqueshavedevelopedSOfar，fewareeffectivetodetectthecanc盯intheearlystageDomesticandovorseasscholarshavepaidmoreandmorea：tte娃tiontofluorescencespectroscopyforitsadvantagesofrealtimedetection’convenient，inexpensive，

3、innocuityandSOonInthisthesis，fluorescencespectroscopyis吣edtoinvestigatetheavailabilityofCanCeridentificationWedetectedautofluorescencespectroscopyofcellandsaulnbyprecisespectrographandhaveachievedgoodresultHepaticcarcino

4、macell(SMMC7721)andhepaticcell(HL一7702)Werecultun覓iIlvi們andtheirautofluorescenceWaSdetectedbyfluorescentspectrographItpresentedthatthespectrogramofthehepaticcarcinomacellWaSresembleasthatofthehepaticcellattherangeofwavel

5、engthfrom550ntoto720ntocharacterizedby咖pea：kSat602nmand691nmButFrom721nmto800nm，thespectraofcaremomacellwittIapeaklocatedat734nmWerefoundForthehepaticcellthepeakat734nmWasnotobservedTheexperimentationsofpropagationoftheb

6、othcellsconfirmulteriorlythatthereisasignificantdifferencebetweentwocells’spectrumTostudythespectroscopyofserum，wecollected87cancerouspatientsand20no珊a1human’sserumanddetectedtheirautofluorescencespectroscopyTheresultsin

7、dicatedthattwokindsofserum’sspectroscopyhavenotabledifference，thepeakintherangeofwavelengthfrom620nmto630nmisthecharacteristicofcanceroDsserum’sspectroscopyInthecourseofresearchthespectroscopYof11cancerouspatients’serumw

8、hoareunderradiotherapyandchemotherapytreatment，wefoundaRertreatmenttheintensityofcharacteristicpeakismireandmoreweakThespectraofporphyrinIXalsobeenstudied，theresultindicatethecharacteristicspectrumofcancerrootinsensitize