三種禾本科牧草高效聯(lián)合固氮菌的篩選和微生物接種劑的研制.pdf

1、甘肅農(nóng)業(yè)大學碩士學位論文三種禾本科牧草高效聯(lián)合固氮菌的篩選和微生物接種劑的研制姓名：席琳喬申請學位級別：碩士專業(yè)：草業(yè)科學指導教師：張德罡20050601Summarysomeadvanced㈣entswereappliedintheresearch，suchasgaschromatograph≤國2》the。fiRccnnitrogenanalyserwithopticalprinciplefordeterminationofstab

2、leisotopos(NOl7)ufiraviolctspectrophotometoretcThetechniqueofMPN，thcmethodofthefifteennitrogenofisotopedilutionandSalkowskicolorimetrywerealsousedAssociativenitrogenfixationbaeterias(ANFB)wereseparatedfromsoilawayfromroo

3、ts(NRS)，rhizoplanandsurfaceofroots(RP)andsoiladi犯ringroots(aS)ofOat(avinasatiav)，smoothbromegrass(Bromusinermls)andhighlandbarley(HordeumvulgareL、TenassocimivenitrogenfixingbacteriaswereisolatedfromturfgrassinGansuprovin

4、ceandShanxiprovincebyusingspecialmediums(CCM，NFM)。Theabilitiesofbacteriaincluding：fixingnitrogenproducingIAAsolubilizin_gphosphorusandworkingintubeweredeterminednlestrainsseparatedfromOatandf刃話atwerereinoculatedtOOatands

5、moothbromegrassthentheeffectofpromotionweredeterminedTheamountofbiolD’gicalnitrogenWasdeterminedbyisotopedRufion鎬wellTheresultsare8sfollows：1Associativenitrogenfixationbacterias(ANFB)wcrcseparatedandenumeratedfromsoilawa

6、yfromroots(NRS)，rhizoplanorsurfaceofroots(RP)aodsoiladheringroots(RS)ofOat，smoothbromegrassandhiglllandbarley56strainswerefromoat，62sRainsWerefromoatand48strainsWerefromoatMoststrainsarefromrhizoplanorsurfaceofroots，less

7、fromsoiladheringrootsandsoilawayfromrootsnlerateofRPfromoathighlandbarleyandsmoothbromegrassare9131％，9019％，8271％respectivelyRSarc6800，741％，1148％andtherateofNRSare：189％，240％，581％Thetotalamountofoatandhighlandbarleyarelarg

8、erthansmoothbromegrass2Associativenitrogenfixingbactcriaswereisolatedfromturfgrassandforage(oat)inOansuprovincebyusingspecialmediums(CCM，NFM)mabilitiesofbacteriaincludingni209en：fixationproducingIAA，phosphorussolubilizin

9、gandworkingintube，weredeterminedTheresultsslmwedthatnineinelevenANFBstrainscouldproduceauxin(IAA)，IAAconcentrationwithLTrpreached216ug／ml～223ug／ml(ninestrainsover10uggn[11)IAAwithoutLTrpreachedO81ug／ml～138ug／ml(sixstrain

10、sover10ug／m1)；sixcandissolveorganicphosphorus，thl“eecandissolveinorganicphosphorus(phosphatesolubilizingpOWerreaching58ug／ml～82ug／m1)，onehasdoublefunction：twohavenopositiveeffectin糙g瓴碡j!e，othershavepositiveeffectShootlen

11、gthincreased628％～1428％，chlorophyll278％～6389％，Abovedryweight2145％～15048％’Underdryweight628％～1428％，’rotallengthofroot227％～4127％，Areaofroot1132％～849％；1017and1012werebetterinproducingauxin1016wasbetterinsyntheticabilityTheyc