清肝開竅祛風法對電點燃模型大鼠c-fos蛋白的表達及細胞凋亡的影響.pdf

1、成都中醫(yī)藥大學博士學位論文清肝開竅祛風法對電點燃模型大鼠cfos蛋白的表達及細胞凋亡的影響姓名：楊東東申請學位級別：博士專業(yè)：中醫(yī)內科指導教師：謝春光20060401AbstractObjectives：Tofurtherexploreanimprovedmethodofkindlingwithrapidlyrecurringhippocampalseizures：TofurtherexploretheeffectsofNingxian

2、decoctiononconvulsioninepilepsyandtheexpressionofClosproteininhippocampalseizuresofthemicewithexperimentalepilepsyinducJbythemethod。Methods：Chronicseizureofepilepsywasinducedbyanimprovedmethodofkind]ingwithrapidlyrecurri

3、nghippoeampalseizureswiththeweightof230—3009，andthemiceat4，5degreewereselectedastheobjeetiveofstudyThemicewithexperimentalepilepsywererandomzedinto5groups：modelgroup，westerngroup，Ningxianhighdosagegroup，Ningxianmiddledos

4、agegroup，andNingxianlowdosagegroup，another6normalmicewereselectedasthecontrol，followingexperimentswereproceededrespectively：firstly，thestudyofbehaviorwasperformdSecondly，28daysaftertheropy，evokepotentialepilepsy，atthesam

5、etimerecodECGThirdly，ABCimmunohistochemicaldecorationmethodWaSadoptedtostudytheexpressionofCfosproteininthehipporegionFinally，quantitativeimageanalysiswascarriedoutwithmedicalimageanalysiSsystemResults：1Thebehaviorstudys

6、howedthatNingxiandecoctionandVPAcouldeffectivelyinhibittheseizuresinducedbykindlingwithrapidlyrecurringhippocampalseizures(pO05)BetweenNingxianlowdosagegroupandthethreegroupsweresignificantdifferent(pO05)3Immunohistochem