有機(jī)磷降解菌的構(gòu)建與微生物肥料的研究.pdf

1、浙江大學(xué)博士后學(xué)位論文有機(jī)磷降解菌的構(gòu)建與微生物肥料的研究姓名：胡秀芳申請(qǐng)學(xué)位級(jí)別：博士后專業(yè)：微生物學(xué)指導(dǎo)教師：陳集雙20030701charactericsofbothionsandbacteriaSporeswerelesssensitivethannormalbacteria，andcapsuleprotectsporesfromseveredamageIonsenhancedlethalratewithincreasingof

2、ions，butnodifferenteffectwerefoundbetweenionswithvariousspeciesandenergyThelethalrateof曠with101510拍ions／cm2onKNP414were60％～95％Themutant021120degrade25％phytateacidinNBRIPmediumandthedegradationrateincreasedto475％inrevised

3、NBRIPmediumTheconstitutionofrevisedmediumwere：glucose10％，(NH4)2S04O2％，sodiumphytate02％，KClO05％，MgS04’7H20005％，MnS04‘H200001％andFeS047H200001％，CaC0301％，pH70。021112wasanotherphytateaciddegradationbacteriaisolatedfromsoilBa

4、sedonphenotypiccharacteristicsandphylogeneticanalysis，021112wasidentifiedasEnterobactersakazakiiwith972％identity021112obviouslydegradephytateacidandlecithinwiththedegradationrateof8709％and8740％respectively021112alsodegra

5、dedphytateacidinrapeseedmealandswinemanureto2604％and1538％respectivelyHighalkalinephosphatasewasfoundinculturemedium，whichindicatedthepossibleactionofphosphataseindegradationoforganophosphateHoweverthecharacteristicsofpho

6、sphatasewereinfurtherstudy2耵leresearchofmicrobialfertilizerAshigheffectivepotassiumandphosphatedissolutionsilicatebacteriatheCultureTechnologyofKNP414wasstudiedAstotheresultsofsinglefactorexperimentstheoptimumculturewas3

7、％starchO2％yeast01％phosphateandO1％MgS04Orthogonalexperimentindicatedthattheoptimumcombinationwas1％starch02％Yeast01％Na2HP4and01％MgS04TheconditionsofculturewerealsoinvestigatedinflaskTheoptimumconditionsin500mlflaskwereasfo

8、llows：50mlmedium，02％CaC03，510％inoculum，250rpm／minrotatespeedUndertheoptimalmediumandfermentationconditions舯414wasculturedin70Lfermentorandtheproductwascertifiedtoberegularbyrelateddepartmentofgovernment3Theeffectofmicrob