擬南芥花粉原生質體質膜陰離子通道的鑒定及其在花粉萌發(fā)和花粉管生長中的作用.pdf

1、中國農業(yè)大學碩士學位論文擬南芥花粉原生質體質膜陰離子通道的鑒定及其在花粉萌發(fā)和花粉管生長中的作用姓名：任永紅申請學位級別：碩士專業(yè)：植物學指導教師：武維華20030601A8S罩RAC善Pollengerminationaswellaspollentubegrowthisanexcellentmodelforthestudyofsignalingmechanismsintipgrowingcells。Inadditiontocalciu

2、mandpotassiumionsthathavebeendemonstratedtoparticipateregulationofpollengerminationandtubegrowth，ithasbeenalsoproposedthatchannelmediatedaniontranspo撞acrosstheplasmamembranemig融beinvolvedintheprocessesThefocusofthisthesi

3、sworkistoidentifyandcharacterizeanionchannelsintheplasmamembraneofArabidopsispollensandtheirsignificanceinregulation0fpoUengerminationandtubegrowthResultsofinvitropollengerminationandtubegrowthshowedthatanionchannelblock

4、ers(DIDS，NFA，NBB羚significantlyinhibitedpollengerminationandtubegrowthTheexternalpHsignificantlyaffectedpollengerminationandtubegrowth，andtheadditionofCI。inthemediumreducedthealkalinepHinducedinhibitionofpollengermination

5、andtubegrowthByapplicationofpatchclamptechniques，thevoltage—dependentinwardcurrentsthatmainlyaccountforanioneffluxwererecorded露’echannelswe∞permeable|。CIandS04。WhenCI’wastheonlychargecarryingioninthepipettesolution，thewh

6、olecellcurrentsshowedrundownrapidlyWhiteCI‘waspartiallysubstitutedwiths04’，therundownofthewholecellcurrentswasnolongerdetectableThewholecellcurrentswereinhibitedbyanionchannelblockerNPPBandalsoinhibitedbyalkalineexternal

7、pH，itisconcludedthatanionlaanspo戢thrc噸】l酶theoutwardanionchannelsidemifiedfromtheplasmamembraneofArabidopsispollenprotoplastsissomehowinvolvedinregulationofpollengerminationandtubegrowthActivityoftherecordedanionchannelsi