瓜蚜侵染對黃瓜葉片次生代謝物質及相關酶活性的影響.pdf

1、山東農業(yè)大學碩士學位論文瓜蚜侵染對黃瓜葉片次生代謝物質及相關酶活性的影響姓名：季春梅申請學位級別：碩士專業(yè)：蔬菜學指導教師：曹辰興20110614瓜蚜侵染對黃瓜葉片次生代謝物質及相關酶活性的影響2AbstractMelonaphidisoneofthemainpestsofcucumber.Chemicalcontrolisamajmeasureonproductionitnotonlyincreasesthecostofproduct

2、ionproductresiduebutalsopollutestheenvironmentsoitisveryimptanttobreednewcultivarwhichcanresisttoaphid.Glabrouscucumbertheirhybridwereusedtocomparetheirdifferentresistantabilitiestoaphid.Therelationshipbetweenthesecondar

3、ymetabolitesseveralcrelativeenzymesofleaveswerestudied.Thisstudywilllayafoundationfthefurtherstudyprovideinfmationftheionbreedingofnewcultivar.Italsocanprovideatheyfstudyingresistancetoaphids.Themainresultswereasfollows：

4、1Anaturalfieldsurveywascarriedoutonthreecucumbermaterialstocomparetheirresistancetoaphid.GlabrouscucumberwasahighaphidresistantmaterialnmalcucumberwasasusceptiblematerialthecapacityofF1toaphid–resistancewasbetweenitspare

5、nts.2Duringtheprocessofinvasionbyaphidtheactivitiesofmetabolismrelatedenzymesincreasedbyindifferentdegrees.Comparedwithnmalcucumbertheactivityofaphidresistancetypesincreasedrapidlyhadhighpeakitsresponsewasrapidtoo.Thestr

6、engthofinducedresistancetoaphidwasdifferentbetweenthedifferentgenotypes.Befeinfectedbyaphidenzymesactivitywasnotsignificantlydifferent.Enzymeactivitywasallincreasedafterinfectedbyaphidsaphidresistancematerialhadhighlevel

7、ofresistancetoaphidthansusceptiblematerial.ThechangesofSODCATactivityindicatedthattheyhadinfluenceonresistancetoaphid.PPOPALactivitiesonglabrousF1werealwayshigherthannmalcucumberPODPPOPALactivityincucumberseedlingleavesi

8、ncreasedfirstthenshowedadownwardtrend.ThusintheprocessofmelonaphiddamagePALPPOactivityinthecucumberwereclosedrelatedtotheaphidresistanceofcucumbersotheycanbeidentifiedasthechemicalindicats.3Inducedbythemelonaphidthemetab

9、olitecontentincreasedindifferentlevels.Thecontentofligninflavonoidsincreasedfirstthenshowedadownwardtrend.Tannintotalphenolcontentexpressedasalternatingrisefall.Afterinoculationofmelonaphidthecontentoflignininglabrouscuc

10、umberwas14.3%128.5%higherthanthatofF1cucumbernmalcucumber.Theaccumulationoftotalphenolsinglabrouscucumberwas24.2%4.9%higherthanthatofnmalcucumberF1cucumber.TheaccumulationofflavonoidstanninsinF1cucumberwashigherthantheot