轉(zhuǎn)Bt基因水稻對倉儲害蟲的影響及其毒理分析.pdf

1、華中農(nóng)業(yè)大學碩士學位論文轉(zhuǎn)Bt基因水稻對倉儲害蟲的影響及其毒理分析姓名：楊妮娜申請學位級別：碩士專業(yè)：植物產(chǎn)品安全生產(chǎn)與質(zhì)量管理指導(dǎo)教師：張國安20090601華中農(nóng)業(yè)大學2009屆碩士學位論文AbstractToevaluatethebiosafetyoftransgenicBacillusThuringiensis(Bt)rice(followedasBtrice)duringthestorage，aserialofstudieso

2、nBtriceexpressingCrylAc／CrylAbgeneanditsparentrice(contr01)wl既econductedinthewarehouseofInsectEcologyLaboratoryofHuaZhongAgriculturalUniversityFirst，studiedontheeffectsoftransgenicBtriceonthestoragepestsintherepositionTh

3、encomparedtheerlzymeactivityofwarehouseharmfulinsectsAtthesametime，separationandidentificationofAspergillus，andtheninfestthetransgenicBtriceTheresultsweTeasfollowsInthestorage，theBtricehadinfluenceOllthegrowthanddevelopm

4、entofSitophiluszeamais，buthadnonegativeeffectontheTriboliumcastaneumInallthetreatmentsthesurvivalrateofSitophiluszeamaisintheBtricehadsignificanthigherthancontrol，asfortheTriboliumcastaneum，betweentheBtriceandit’Scontrol

5、hadnosignificantdifferenceAccordingtotheanalysisofenzymeactivity，theresultsindicatedthatbesidestheCarEofSitophiluszeama話inSYnonBtricehadhigherthaninSYBtriceandtheSODofTribo廳“研castaneuminSYnodBtricehadhigherthaninSYBtrice

6、，intheothertreatments，theCarE，TchE，ADH，SOD，a11theenzymeactivitiesinBtricehadhigherthancontr014kindsofpathogenicfungiWasisolatedfrommoldyrice，observetheconfigurationandthroughtheribosomeDNAITSsequenceanalysis，theresultssh

7、owedthatallthe4kindsofpathogenicfungiweretheAspergillusgavusInoculationeffectwasstudiedwithintegrated，cutembryokernelsofBtricevaritiesandnonBtricevaritiesinoculatedwithAflawraceatdifferentsporeconcentration，theresultsrev

8、ealedthattherewassignificanteffectonvarietiesofrice嬲well鵲thekernelswithdifferentdamageddegree；SignificanteffectwasobservedamongthesporeconcentractionofAflavusalsoKeywordsTransgenicBtrice；storagepests；enzymeactivity；Aflav