水稻雄性不育突變體Osms18的表形觀察及基因定位的研究.pdf

1、寧夏大學(xué)碩士學(xué)位論文水稻雄性不育突變體Osms18的表形觀察及基因定位的研究姓名：黎雯雯申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：張大兵20090301AbstractRiceisoneofthemostimportantcropsMalesterilityplaysallessentialroleinheterosisperformanceClmfythemechanismunderlyingantherandpollen

2、developmentcouldgreatlyimprovetheusageofheterosisinricebreedingTheOsmsl8mutationwasselectedfromaM2populationthroughmutagenizingricecultivar9522with叱oYrayGeneticanalysisindicatedthattheOsmsl8malesterilityphenotypewascontr

3、olledbyasinglerecessivenuclearlocusObservationofthetissuesectionsshowedthattheOsmsl8mutantshavetwokindsofphenotypesBothofthemproducedabnormaltapetum，andpollenswithbiggergermporeandthickerpollenexinePollensdisplayingmildp

4、henotypecoulddeveloptomaturestagewithtweproductivenuclearsandstarchgranulesPollensofseverephenotypecouldnotaccumulatestarchgranulesandcollapsedaftervacuolatedstageTomapOsMSl8locus，twoF2populationwereconstructedseparative

5、lyfromthecrossbetweentheOsmsl8(japonica)andGUANGLUAI(indica)Osmsl8(japonica)and9311(indica)TheOsMSllocuswasfirstlylocalizedbetweenthetwolnDelmolecularmarkers，0S213andOS216onchromosome2TofurtherlocatetheOsMSl8locus，sixnew

6、lndelandSNPmarkersweredevelopedThelocuswasrecentlyrestrictedtoaregionof47kbphysicaldistance，boundarybyL1andL3markerTheseresultsprovidehelpfulinformationforthefurthercloningandfunctionanalysisofOsMSl&Keywords：Or)gasativa，