利用LA1777和LA2951漸滲系對番茄抗晚疫病QTLs進行定位及番茄晚疫病菌侵染葉片細胞學觀察與比較.pdf

1、內(nèi)蒙古農(nóng)業(yè)大學碩士學位論文利用LA1777和LA2951漸滲系對番茄抗晚疫病QTLs進行定位及番茄晚疫病菌侵染葉片細胞學觀察與比較姓名：王豐申請學位級別：碩士專業(yè)：蔬菜學指導教師：云興福李君明20090501QTLindentificationconferringPhytophthorainfestansusingintrogressionlinderivedfromLycopersiconto1?！甃A1777mtroesslonli

2、nederivedtromLycopersicoumhirsutumA1／／andSolanumlycopersicodeLA2951andcytologyofresistanceoftomatotolateblightAbstracthlthisstudyweused93introgressionlinesderivedfrom三hirsutumLAl777together、們也E6203and77introgressionlines

3、deftvedfrom&lycopersicodeLA295ltogether、^，itllVF36tomapandcharacterizequantitativetraitloci(QTLs)conferringresistancetolateblightAtthesametime，weusedthecytologicalmethodstofollowinfectionoftomatoleavesbyPhytophthorainfes

4、tansnlemainresultsobtainedinthisresearchwereconcludedasfollowing：(1)Microscopicanalysessuggestthat：First，weCallfollowtheprocesswhichsporangiumswereplacesonthesurfaceoftheleafformedgermtubesandeventuallygermtubesthroughep

5、idermisstomataandcellin48hafterinoculationusingsamplesafterthestainingprocedureSecond，therealeextremelysignificantlesssporangiurnsformedgermtubesontheleafsurfaceoftomatomaterialswitll砌2genesthanofcultivatedspeciesM82in14

6、hafterinoculationThereareextremelysignificantlesssporangiumsformedgermtubesontheleafsurfaceoftomatomaterialswithPh一2、Ph一3genesthanofcultivatedspeciesM82in38hafterinoculation(2)Weused93introgressionlinesderivedfrom三hirsut

7、umLA1777togetherwithE6203togain5QTLsnamedhgm4—1，hgm42，hgm7—1，hgm8—1，hgml21showedtheresistancetolateblightTheseQTLswerelocatedonchromosome4，7，8，12respectivelyAmongthem，oneQTLshgm7一lonchromosome7gavemoreresistancethantheot

8、herTheareaofthediseaselesionwasreduced2525％comparingtoE6203afterinoculation(3)Wefoundinoculationdataisnormaldistribution，SOthetraitoftomatoresistancetolateblightisthequantitativetraitAnd3QTLsnamedsgm21，sgm41，sgm91showedt