2023年全國碩士研究生考試考研英語一試題真題(含答案詳解+作文范文)_第1頁
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1、Rice (Oryza sativa L.) is one of the most important food crops grown widely throughout the world, providing a staple food for nearly half of the global population.Pest and disease problems are major constraints for incre

2、asing rice production in almost all parts of the world.The increasing awareness of detrimental effects on the reliance of chemical based management practices in the past has transformed the direction of research efforts

3、towards developing a more environmental friendly and economically feasible management practices on crop production.As a novel approach, Bt rice plants containing cry genes have been successfully developed for the managem

4、ent of lepidopteran pests of rice.Although transgenic rice varieties have not been approved for commercial cultivation any part of the world yet, China is likely to soon commercialization of Bt rice.However, the impact o

5、f Bt rice on non-target herbivorous pest insects are necessary parts of an environmental risk assessment studies, that should be undertaken before commercialization to ensure safety consideration for Bt rice.For example,

6、 although the brown planthopper (BPH), Nilaparvata lugens (St(a)l), is an important pest of rice worldwide, the impacts of transgenic Bt rice on the non-target BPH are poorly understood.Therefore, non-target impacts of t

7、hree transgenic Bt rice lines (expressing the cry1Ab/cry1Ac, cry1Ca and cry2Aa proteins) on the BPH, were evaluated.Cellular immune system responses were assayed with a detailed analysis of total hemocyte count (THC), di

8、fferential hemocyte count (DHC) and histopathology of hemocytes of BPH across three consecutive generations.Given that the morphological characteristics of BPH hemocytes were unknown before this study, an attempt was mad

9、e to investigate the detailed microscopic analysis of the hemocytes of BPH.Expression of the phenoloxidases activation system in BPH fed on Bt rice plants were studies as phenolxidases served an indicator of intensity of

10、 the insects immune reactions.In particular, abundance of the yeast-like symbiotes (YLS) in BPH fed on different Bt rice and non-Bt parental control was monitored.Moreover, the molecular responses of digestion, detoxific

11、ation and immune response genes of BPH were elucidated for the better understanding the ability of the BPH to adapt the Bt rice plants.Fitness consequences of BPH fed on Bt rice plants were observed by monitoring fecundi

12、ty,survival, growth and development, indicating a significant cost to the adaptation of BPH to Bt rice.In addition, proteomic analysis was performed to identify the Bacillus thuringiensis toxin binding activity in the BP

13、H fed on Bt rice plants. Examination of hemocytes from BPH indicated the presence of six types of hemocyte:prohemocytes (PRs), plasmatocytes (PLs), granulocytes (GRs), adipohemocytes (ADs),oenocytoids (OEs) and spheruloc

14、ytes (SPs) and to the best of our knowledge, this is the first study to detect the hemocytes of BPH.It was estimated that the mean numbers of total haemocyte count are varied from 6096.4 to 7393.6 cells per mm3 of haemol

15、ymph with the different physiological stages of the insects and it was statistically increased in the aged adults.PLs, GRs and PRs are the most abundant cells in the haemolymph in all development stages of BPH while SPs

16、is the scarcest type.The results revealed that transgenic Bt rice expressing Cry1Ab/Cry1Ac, Cry2Aa and Cry1Ca had no significant adverse effects on total hemocyte count, differential hemocyte count and phenoloxidases act

17、ivation of BPH, indicating that Bt rice could not affect the hemocytes and PO activities of BPH.No significant differences were observed on the population abundances of YLS in BPH, which were fed on Bt rice compared to t

18、he non-Bt parental rice.The results revealed that the tested Bt rice strains have no unfavorable effect on fecundity,survival and growth of BPH.Furthermore, no differences were observed in tested gene expression among th

19、e BPHs fed on Bt rice lines and their non-transgenic parental cultivar,thus, provides the conclusion that Bt rice have no detrirnental effects on the physiological processes of digestion, detoxification and immune respon

20、ses of BPH due to our data.The the western blot analysis showed that the materials reacting with cry 1Ac and cry 1Ca antibodies were present in crude homogenates from adult BPH, which had been fed on Bt rice lines, but,

21、the interaction between cry toxins and gut receptors of BPH are complex and need further investigation at molecular levels to confirm the functional receptors for cry toxins in BPH.This study enhances understanding of th

22、e complex interaction between transgenic rice and BPH.The new findinds would be useful for understanding the effects of Bt rice on non-target sap sucking insects and it is hoped that this will serve as a more reliable pr

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