pm2.5不同成分在體染毒對大鼠腎血管環(huán)收縮舒張反應及nosno的影響

1、山西醫(yī)科大學碩士學位論文PM2.5不同成分在體染毒對大鼠腎血管 環(huán)收縮-舒張反應及NOS/NO的影響姓名：孫萌申請學位級別：碩士專業(yè)：心血管內科指導教師：呂吉元2011-05-10山西醫(yī)科大學碩士學位論文 II Effects of three components of PM2.5 on renal artery to vasoactives and serum level of NOS/NO Abstract Objectiv

2、e To investigate the effects of administration of water-soluble or liposoluble of PM2.5 on myogenic responses of renal artery to vasoactives and to explore the underlying mechanisms. Methods Water-soluble, liposolub

3、le, mixture of the both of PM2.5 were administrated intravenously successively for 10d. DMT and Power-Lab system were used to record the tone of the renal artery rings. ELISA was used to measure the serum level of NOS/NO

4、. Results 1.Compared with the control group,no significant difference was found in the renal artery rings contractions induced by 60mM KCl and U46619 among the four groups (P＞0.05). 2.Compared with the control group,Ace

5、tylcholine induced vasorelaxation was attenuated in PM2.5 extracts-treated groups(P＜0.05). 3.Compared with the control group, Nitroprusside sodium induced vasorelaxation was attenuated in PM2.5extracts-treated groups，has

6、 no significant difference(P＞0.05). 4.Serum NO level was increased in PM2.5 extracts-treated groups (P < 0.05). Serum eNOS level significantly decreased in the mixture treated group (P＜0.01)，but no significant differe

7、nce was found in water-soluble or liposoluble treated group (P＞0.05). Serum iNOS level increased in all PM2.5 extracts-treated groups, compared with the control (P ＜ 0.05). Serum AngⅡ level decreased in all PM2.5 extract

8、s-treated groups, compared with the control (P＜0.05). Conclusion 1.Administration intravenously of PM2.5 extracts enhanced the renal artery rings contractions induced by 60mM KCl and U46619 among the four groups,but ha

9、ve no significant difference . 2.Administration intravenously of PM2.5 extracts attenuated the vasorelaxation response of renal arteriole to acetylcholine. 3.Serum NO level was increased in PM2.5 extracts-treated groups.

10、 4.Serum eNOS level significantly decreased in the mixture treated group，but no significant difference was found in water-soluble or liposoluble treated group. 5.Serum iNOS level increased in all PM2.5 extracts-treated g

11、roups, compared with the control. 6.Serum AngⅡlevel decreased in all PM2.5 extracts-treated groups, compared with thecontrol. 7.The effect of three components of PM2.5 to vascellum has related with oxidative stress injur