棒曲霉素和辣椒素的魯米諾化學(xué)發(fā)光行為研究及應(yīng)用.pdf

1、西北大學(xué)碩士學(xué)位論文棒曲霉素和辣椒素的魯米諾化學(xué)發(fā)光行為研究及應(yīng)用姓名：劉厚勇申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：宋正華20090609AbstractThethesisfocusesontheresearchandapplicationofpatulinandcapsaicinchemiluminescence(CL)behaviorwithvariousluminolCLsystemsThemechanismsandappli

2、cationsofvariousCLsystemsforthefoodandbiologicalanalysisweresimplyreviewedusing138referencesconcerningCLanalysis，basedonthefundamentaltheoryofchemiluminescence(CL)andcombinedwithmyresearchworkPatulin，4hydroxy4Hfuro[3，2c]

3、pyran2(6H加ne，isformedinapplesdecayedbycertaingenusofmolds，particularlyPenicilliumexpansum，easilytransfersintoapplejuiceduringprocessingowingtoitssolubilityinwaterCarcinogenic，teratogenicandmutageniceffectsofpatulinhavebe

4、enreportedTheWorldHealthOrganizationrecommendslimitingitscontentsinfoodsto50pgkg1Inthiswork，itWasfoundthatpatulincouldenhancetheCLintensityproducedbyluminolandhydrogenperoxideinalkalinemediumThesystemrespondedlinearlyint

5、heconcentrationrangefromO05to800ngmEmlwiththedetectionlimitof001ngmL‘(30)TheproposedmethodwassuccessfullyappliedtothemeasurementofapplejuicesamplesItWasfoundthatpatulincouldinhibittheCLgeneratedfromluminolanddissolvedoxy

6、gensystemsignificantlyThedecrementofchemiluminescenceintensitywaslinearwiththepatulinconcentrationovertherangefrom004to100ngmL1(尸=09989)，withthedetectionlimitof001ngmEml(30)Theproposedmethodhasbeenappliedsuccessfullyinth

7、edeterminationofpatulininapplejuiceCapsaicin(trans8methylv卻anillyl_6邶onenamide)istheprincipalalkaloidderivedfromcapsicumfruitextractsofdifferentofCapsicumspeciesCapsaicinhasmanypharmacologicaleffectssuchasanalgesic，antii

8、nflammatory，promotingappetite，improvedigestion，andantibacterialinsecticideBasedontheinhibitoryeffectofcapsaicinonthechemiluminescence(CL)reactionbetweenluminoldissolvedoxygen，luminolmyoglobin，luminolK104andluminolH202inf