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簡(jiǎn)介：福建農(nóng)林大學(xué)碩士學(xué)位論文多花水仙若干品種類(lèi)型親緣關(guān)系的分子生物學(xué)研究姓名吳菁華申請(qǐng)學(xué)位級(jí)別碩士專(zhuān)業(yè)生化與分子生物學(xué)指導(dǎo)教師呂柳新20040401福建農(nóng)林火學(xué)壩_學(xué)位論義摘要水仙II一2是II一1的同源三倍體提供了佐證。4水仙單染色體微分離與微克隆1水仙I1的根尖為材料，制備出用于微分離的染色體標(biāo)本，利用顯微操作系統(tǒng)，采用粘取法成功分離出I1中一個(gè)特殊的中著絲粒的染色體。2利用LAPCR方法成功地對(duì)該單染色體進(jìn)行了體外擴(kuò)增，獲得了片段大小為5002500BP的水仙中著絲粒染色體DNA庫(kù)。3用PGEMTEASYVECTOR克隆PCR產(chǎn)物，構(gòu)建了單染色體的DNA質(zhì)粒文庫(kù)。隨機(jī)分析部分重組子，發(fā)現(xiàn)文庫(kù)的插入片段大小為6002500之間。關(guān)鍵詞水仙；過(guò)氧化物酶同工酶；隨機(jī)擴(kuò)增多態(tài)性DNA；熒光原位雜交；染色體微分離單染色體DNA質(zhì)粒文庫(kù)；

簡(jiǎn)介：寧夏大學(xué)碩士學(xué)位論文六盤(pán)山瀕危植物桃兒七生物學(xué)特性及其資源保護(hù)的研究姓名楊麗琴申請(qǐng)學(xué)位級(jí)別碩士專(zhuān)業(yè)植物學(xué)指導(dǎo)教師王俊20090301ABSTRACTTHISPAPERDESCRIBESTHEPHENOLOGY,HABITATANDREPRODUCTIVEBIOLOGYOFENDANGEREDSPECIESOFSINOPODOPHYLLUMHEXANDRUMROYLEYING，INTHEINVESTIGATIONOFTHESURVIVALSITUATIONOFSINOPODOPH7HUMHEXANDRUMROYLEYINGINLIUPANMOUNTAIN，THEANALYSISOFTHEEXTINCTIONREASONSALEDISCUSSED，INEXTINCTIONOFSINOPODOPHYLLUMHEXANDRUMROYLEYING,WEDOTHEDOMESTICATIONANDARTIFICIALKEYINTHENATURALPROTECTIVEDEVELOPMENTANDCHANGE，APPLIEDRESEARCHINVITROSTUDIESTOEXPLORETHEWAYSOFCULTIVATINGARTIFICIALSEEDLINGCULTIVATIONOFSINOPODOPHYLLUMHEXANDRUMROYLEYING，ANDPUTFORWARDTHEEFFECTIVEWAYSOFRESOURCESPROTECTIONANDSUSTAINABLEUTILIZATIONCOUNTERMEASURESOFSINOPODOPHYLLUMHEXANDRUMROYLEYING．THERESULTSWEREASFOLLOWS1．INLIUPANMOUNTAIN,SINOPODOPHYLLUMHEXANDRUMROYLEYMGOFTENGROWSABOVE2000MOFTHETREESANDBUSHES。FOCUSONWESTGORGE、REDGORGE、CASTSLOPE、SUTAI、THETWODRAGONSFIVER、SMALLSOUTHAREA、COOLHOUSEGORGE、BIGSLIOWMOUNTAIN．NECOLDWETCLIMATESDISTRIBUTION．ANNUALAVERAGETEMPERATUREIS2．32．5DEGREES，THEAVERAGEANNUALRAINFALLIS700MM,RELATIVEHUMIDITYIS68％．LOWTEMPERATUREDISTRIBUTION，ANDTHEBIGWINDISTHEMAINCHARACTERISTICSOFTHEECOLOGICALENVIRONMENT．2．SINOPODOPHYLLUMHEXANDRUMROYLEYINGISPERENNIALHERBS，INLIUPANMOUNTAIN，SINOPODOPHYLLUMHEXANDRUMROYLEYINGUSUALLYBREAKSINEARLYORMIDDLEMAY,ANDBLOOMSINLATEMAYOREARLYJUNE，F(xiàn)LOWERS，WHICHAFEBLOSSOMPOLLINATION，AREBIGGERTHANLEAVES，THELEAFRAPIDGROWTHINJUNEWHICHISTHERAINYSEASON，F(xiàn)RUITRIPESINSEPTEMBERANDOCTOBER,NOVEMBER,THEPARTOFUNDERGROUNDTODIEINTHEFORMOFDORMANCYBUDS．BUTTHESEEDONNATURALCONDITIONSNEEDTOABOUT10MONTHS，THENTHEDORMANCYANDGERMINATION，INTERMITTENTPIECEMEALSEEDGERMINATIONANDTHEREFORESURIALRATEISLOWER．3．THESEEDOFSINOPODOPHYLLUMHEXANDRUMROYLEYINGINNATURALCONDITIONSHAVELONGDORMANCYANDEASYTOLOSEENERGYPARASITIZATIONOFPHYSIOLOGICALCHARACTERISTICS，NEED8TOPASSFORALONGTIMEAFTERCOMPLETINGTHEDORMANCY．BASEDONCHARACTERISTICSOFSINOPODOPHYLLUMHEXANDRUMROYLEYINGSEEDGERMINATIONOFRESEARCHSHOWSTHATH2S04OFSINOPODOPHYLLUMHEXANDRUMROYLEYINGETCHED誦THGA3SOAKED24HCANBREAKSINOPODOPHYLLUMHEXANDRUMROYLEYMGSEEDDORMANCY,IMPROVINGSEEDGERMINATIONRATE．4．，11LETISSUECULTURERESEARCHOFSINOPODOPHYLLUMHEXANDRUMROYLEYINGSHOWSTHATTHESEEDHAVEBETTERCALLUSINDUCTIONRATETHANLEAFANDPETIOLES，SUITABLEFORASEPTICSEEDLINGINDUCTIONOFOPTIMALMEDIAISMSNAA0．2M∥L6BA0．5MG／L，SUITABLEFORASEPTICSEEDLINGPROLIFERATIONMEDIUMISMS6一BAL．0MG／LNAA0．1MG／L，SUITABLEFORCALLUSINDUCTIONMEDIUMISMS2,4DI．0MGN一KTO．2MG／L．SUITABLEFORCALLUSDIFFERENTIATIONMEDIUMISMS6一BA0．5MG／LNAA0．1MG／L．5．DUETOTHENATURALANDMALLMADEDESTRUETION,SINOPODOPHYLLUMHEXANDRUMROYLEYINGSHARPDECREASE，ANDTHEPOPULATIONOFINDIVIDUALSURVIVALEXTINCTION．ACTIVELYCULTIVATINGFENCE，ARTIFICIALMEASURESCANRESTOREPOPULATIONADVANTAGEP,CTIVELYWILDDOMESTICATIONANDARTIFICIALCULTIVATIONFORTHEEFFECTIVEREALIZATIONOFSUSTAINABLEUTILIZATIONOFRESOURCES．TRADITIONALMEETMARKETDEMAND．KEYWORDSSINOPODOPHYLLUMHEXANDRUMROYLEYMG，ENDANGERED，TISSUECULTURE,RESOURCEPROTECTION

簡(jiǎn)介：國(guó)內(nèi)闡書(shū)分類(lèi)號(hào)國(guó)際圖書(shū)分類(lèi)號(hào)東北龍膽農(nóng)學(xué)博士學(xué)位論文密級(jí)GENTIANAMANSHURICAKITAGAWA生物學(xué)研究王臣導(dǎo)師胡寶忠教授申請(qǐng)學(xué)位級(jí)別；農(nóng)學(xué)博士專(zhuān)業(yè)名稱(chēng)作物遺傳育種論文提交日期2005年4月25門(mén)答辯日期2005年5月所在單位東北農(nóng)業(yè)大學(xué)授予學(xué)位單位東北農(nóng)業(yè)大學(xué)學(xué)位授予日期答辯委員會(huì)主席／CA5馳栽培。。。，，，查些些墜蘭垡堡皇墼絲耋。。。。。，。一苦苷含量有明顯差異，泰康我省西部栽培的高于巴彥我省中部栽培的龍膽苦營(yíng)含量在不同個(gè)體和不同變異型之間含量差異顯著，進(jìn)行高含量育種將是切實(shí)可行的。綜合分析本文的研究結(jié)果，作出以下結(jié)論1東北龍膽在抗旱性、抗病性、光合效率、經(jīng)濟(jì)系數(shù)以及藥材質(zhì)量上明顯優(yōu)于粗糙龍膽，其單株生物量較低的不足可以通過(guò)提高栽培密度彌補(bǔ)，因此，東北龍膽應(yīng)該作為按GAP進(jìn)行生產(chǎn)的酋選種質(zhì)。2栽培東北龍膽的遺傳組成為雜合體，與粗糙龍膽白然雜交是產(chǎn)生雜合體的原因之一通過(guò)雜交手段可以將粗糙龍膽和東北龍膽的優(yōu)良性狀組合在一起，創(chuàng)造新品種。另外，東北龍膽栽培群體的豐富的變異同時(shí)表現(xiàn)在形態(tài)上和有效成分含量上，可以通過(guò)對(duì)高含量個(gè)體的篩選實(shí)現(xiàn)栽培種的優(yōu)質(zhì)化。3在沙壤土上栽培東北龍膽，各項(xiàng)指標(biāo)均優(yōu)于在草甸土上栽培，說(shuō)明黑龍江省中西部沙壤土地帶適于發(fā)展東北龍膽的栽培生產(chǎn)。這一結(jié)論給我們的啟示是原生地綜合條件對(duì)于有效成分的積累不一定是最好的，在研究提高產(chǎn)量的栽培技術(shù)時(shí)更應(yīng)重視高質(zhì)量栽培條件的研究。4將龍膽苦菅含量、折干率、可溶性糖和單株重四項(xiàng)指標(biāo)結(jié)臺(tái)在一起，栽培龍膽的最佳采收期應(yīng)在8月末至9月20日之間。關(guān)鍵詞東北龍膽；栽培；生物學(xué)；龍膽苦苷；發(fā)育形態(tài)學(xué)II

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簡(jiǎn)介：寧夏大學(xué)碩士學(xué)位論文12種擬步甲科昆蟲(chóng)生物學(xué)特性研究（鞘翅目）姓名張建英申請(qǐng)學(xué)位級(jí)別碩士專(zhuān)業(yè)農(nóng)業(yè)昆蟲(chóng)與害蟲(chóng)防治指導(dǎo)教師于有志20050301STUDIESOILBIOLOGICALCHARACTERSFORTWELVESPECIESOFTENEBFIONIDCOLEOPTERAABSTRACTDARKLINGBEETLESISOLLEOFBEETLESTHATDISTNBUTEWIDELYANDITSSPECIES，MORPHOLOGYLIFETYPEAREDIVERSILORMEXTREMELY011THEEARTHTENEBRIONIDISINCLOSERELATIONWITHAGRICULTURE，F(xiàn)ORESTRYSTOCKBREEDINGANDSTORAGETHINGSOMEKINDSAMIMPORTANTIESOUICEINSECTSSMDYI丑GITSBIOLOGYHASIMPORTANTTHEORYANDPRACTICEMEANINGTHEDESERTSEMIDESERTREGIONISTHEMAINDISTRIBUTIONAREA；ITSBIOLOGICALSTUDYISGREATDIFFICULTBUTITSBIOLOGICALSTUDYISALSOIMPORTANTTOTHEEXPLOITUREOFRESOUROEINSECTANDCONTRLOFGRASSLANDPESTINTHISAREATHEPRESENTPAPERIS011THEBASEOFOBSERVATIONANDCOGEETIONINTHEFIELDANDBREEDINGINTHELOOMFOR3YEARS，ITISASTUDYOFMORPHOLOGYANDBIOLOGICALCHARACTERSFOR4TRIBES9GENERAWITHTWELVESPECIESOFTENEBRIONIDFROMMONGOLIAXINJIANGREGIONOFCHINAAMONGTHEM，THEBIOLOGICALCHARACTERSPARTISANINITIALREPORTINTHEMORPHOLOGYPARTADULT，EGG，LARVAANDPUPAOFMORPHOLOGICALCHARACTERSOFEACHSPECIESAREDESCRIBEDINTHEBIOLOGICALCHARACTERSPART，THEGROWTHCOLLRSEOFALLFORM，THEGROWTHCOURSEOFLARVALIFECYCLEANDHABITARESTUDIEDTHEPRESENTPAPERDRAWSUP39TABLESANDDEPICTS202MORPHOLOGICALCHARACTERSFIGURESTHETHESISISDIVIDEDINTO7CHAPTERSALTOGETHER1INTHEPREFACE，RESEARCHCONTENT，RESEARCHPURPOSEANDRESEARCHIMPORTANCEOFINSECTBIOLOGYAREINTRODUCEDTHEREVIEWOFCLASSIFICATIONRESEARCHBREEDINGMETHOD，LIFECYCLEANDHABITOFALLFORMOFTHETENEBRIONIDAFCRECOMMENDED2INTHEMATERIALANDMETHODS，RESEARCHMATERIAL，COLLECTIONMETHODANDPRESERVATIONOFSPECIMEN，COLLECTIONRECORD，BREEDINGRECORDALERECOUNTEDTHEN，RESEARCHMETHOD，BREEDINGMETHODANDOBSERVATIONMETHODOFBIOLOGIEALCHARACTERSAMINTRODUCED3THEBIOLOGICALCHARACTERSOFPIMELIINIINSECTANDSTERNOPLAXSETOSASETOSABATES，PWROCOMAMESOQTEROCOMALOCXYIFRIVALDSZKYPTEROCOMAMONGOLOPTEROCOMAREITTERIFRIVALDSZKYANDTRIGNOCERAPSEUDOPIRAELIAREITTERBELONGTOPIMELIMIAIESTUDIED4THEBIOLOGICALCHARACTERSOFAKIDINIINSECTANDCYPHOGENIACYPHOGENIACHINENSISFALDERMANNBELONGSTOAKLDINIISRESEARCHED5THEBIOLOGICALCHARACTERSOFBLAPTINIINSECTANDBLAPSJEMORALISFEMORALISFISCHERWALDHEIM，BLAPSKIRITSHENKOISEMEUOVETBOGATSCHEVANDBLAPSGOBIENSISFRIVALDSZKYBELONGTOBLAPTINIAREINVESTIGATED6THEBIOLOGICALCHARACTERSOFOPATRINIINSECTANDOPAMONSUBARATUMFALDERMANN，PENTHICUSALASHANICAREICHARDT，GONOCEPHALURARETICULATUMMOTSCHULSZKYANDEUMYLADAPOMNINIREITTERBELONGTOOPANIMARESTUDIED7COMBININGTHESTUDYRESULTSOFTHISPAPERWITHTHERESEARCHJSACCUMULATEDINEARLIERSTAGEOFTUTORSSEMINARTHISPAPERCOMPARESLIFECYCLETHEGROWTHCOURSEOFALLFORMTHE／STARSOFLARVAOF9TRIBESFOURTEENGENERAWILHNINETEENSPECIESOFTENEBRIONIDANDSUMMARIZESTHEIRNATURALENEMYFOODILLGDEFELLCE，ACTIVITYRHYTHMSWARMING，REPRODUCTIONBEHAVIOR，LIFESPANANDSEXPROPORTIONOFTHEADULTTHERESULTSAREASFOLLOWS

簡(jiǎn)介：內(nèi)蒙古農(nóng)業(yè)大學(xué)碩士學(xué)位論文一株降膽固醇乳酸菌的篩選及其生物學(xué)特性的初步研究姓名金鑫申請(qǐng)學(xué)位級(jí)別碩士專(zhuān)業(yè)發(fā)酵工程指導(dǎo)教師段開(kāi)紅韓秀萍20080501ACIDTOLERANCESCANNINGANDBIOLOGICALCHRICTRESTICSTUDYONAIACTOBACILLUSABSTRACTWITHIMPROVEMENTOFPEOPLELIVINGSTANDARD，MOREFOODSWEREINTAKEDSIMULTANEOUSLYITWASSHOWEDTHATCEREBROVASCULARANDHEARTDISEASE，SUCHASARTHEROSCLEROSISAS，CORONARYARTERYDISEASECAD，CEREBRALAPOPLEXYWEREMOREUNIVERSALANDWASFIRST1ILLERFORHUMANHEALTHLACTICACIDBACTERIAAREONEOFMAJORMICROBIALPOPULATIONININTESTINALTRACTOFANIMALANDHUMANANDCLOSELYRELATEDTOPHYSIOLOGICALFUNCTIONOFBOTYLOTSOFEXPERIMENTSSHOWEDTHATLABHASFUNCTIONSONCHOLESTEROLREMOVALABILITYINVIVOANDVITROKOUMISSWASTRADITIONALFERMENTEDMILKWITHMOREMEDICALCAREFUNCTIONSOSOMEEXPREMENTSWERECONDUCTEDON9SAMPLESFROMZHENLAN，ABAKACOUNTY，INNERMONGOLA。BIOLOGICIALCHARACTERISTICSANDMICROBIOLOGICALCOMPOSITIONSOF11SAMPLESOFKOUMISSFROMINNERMONGOLIAWEREDETERMINED11STRAINSOFLACTIBUCILLIWEREOBTAINDBYTRADITIONALPHYSIOLOGICALANDBIOCHEMICALISOLATIONANDIDENTIFICATIONMETHOD，INWHICH2STRAINSWAS厶ACIDOPHILUSANDLSTRAINWASLHELVETICUSFURTHERMORE，ONESTRAINWITLLLLIGHACIDTOLARONCEANDBILEACIDRESISTANCECHARACTERISTICWERESCANNEDFROMTHEASESTRAINSANDTHATTHECHOLESTEROLREMOVALABILITYWASCONDUCTEDINVITROTHERUSLTSSHOWEDTHATTHECOUNTSOFLACTICACIDBACTERIAWERE218X105INASAMPLEKUMISS，ASFORTHEOTHER10SAMPLESTHECOUNTSOFLACTICACIDBACTERIAWERE249X108～298X109CFU／MLTHECOUNTSOFYEASTWERE260X106～163X108CFU／MLEXPEREMENTOFACIDTOLERANCEANDBILERELEASEPROPERTYOFTHISSTRAINSHOWED廿1ATRATEOFBILERELEASEBYTHISSTRAINWAS352％NAMELYTHISSTRAINCANTOLERATELOWERPH25，HI曲BILEACIDRESISTANCEPROPERTYANDCHOLESTEROLREMOVALABILITYKEYWORDSKOUMISS；MICROBIALCOMPOSITION；LACTICACIDBACTERIA；ACIDTOLARONCETBILEACIDRESISTANCE；，CHOLESTEROLREMOVALABILITYDIRETTEDBYPROFDUANKAIHONG，PROFH／HNXIUPINGAPPIICANTFORMASTERDEGREEJINXINMAST盯OFFERMENTATIONENGINEERING

簡(jiǎn)介：山東大學(xué)碩士學(xué)位論文粘細(xì)菌SOCE9882子實(shí)體毒理學(xué)研究及生物學(xué)功能的初步評(píng)價(jià)姓名唐慧申請(qǐng)學(xué)位級(jí)別碩士專(zhuān)業(yè)微生物學(xué)指導(dǎo)教師李越中20050513山東大學(xué)碩士學(xué)位論文摘要纖維堆囊菌是粘細(xì)菌中唯一能降解纖維素的屬，粘細(xì)菌是革藍(lán)氏陰性、單細(xì)胞、滑動(dòng)細(xì)菌，它主要存在于土壤、糞便、腐爛的植物材料以及活的或死的樹(shù)干上。作為～種土壤中廣泛存在的細(xì)菌，它可以在營(yíng)養(yǎng)貧瘠的條件下形成子實(shí)體，大量細(xì)胞共周協(xié)作生活，這一特點(diǎn)在原核生物中獨(dú)一無(wú)二。而且，粘細(xì)菌子實(shí)體在形態(tài)和結(jié)構(gòu)方面有不同程度的復(fù)雜性，這一特點(diǎn)與藥用真菌的子實(shí)體有相似之處。到目前為止，關(guān)于纖維堆囊菌的研究工作主要集中在它能產(chǎn)生豐富的次級(jí)代謝產(chǎn)物這～方面，而對(duì)其子實(shí)體的應(yīng)用開(kāi)發(fā)國(guó)內(nèi)外未見(jiàn)文獻(xiàn)報(bào)道。本文的土要目的就是探索粘細(xì)菌中纖維堆囊菌SORANGIUMCELLULOSUM子實(shí)體作為藥用菌材料的可行性和藥用前景。下面是論文工作的簡(jiǎn)要介紹首先。對(duì)纖維堆囊菌SOCE9882子實(shí)體提取物進(jìn)行毒理學(xué)分析，包括經(jīng)口急性毒性試驗(yàn)、AME8試驗(yàn)、小鼠骨髓嗜多染紅細(xì)胞微核試驗(yàn)、小鼠精子畸形試驗(yàn)。通過(guò)實(shí)驗(yàn)可以得出結(jié)論在本次實(shí)驗(yàn)條件下，受試物為實(shí)際無(wú)毒物質(zhì)，未顯示有遺傳毒性作用，可以初步認(rèn)為纖維堆囊菌SOCE9882子實(shí)體提取物是安全可靠的。其次，本文對(duì)纖維堆囊菌SOCE9882子實(shí)體提取物的藥理學(xué)活性進(jìn)行了初步探索。主要做了一個(gè)方面的嘗試，即按照活性多糖的研究方法對(duì)粘細(xì)菌子實(shí)體熱水粗提物進(jìn)一步的分離，同時(shí)對(duì)乙醇回流萃取的脂類(lèi)物質(zhì)按照極性的不同進(jìn)行了初步分離。對(duì)分離到的部分組分進(jìn)行細(xì)胞毒型抗癌藥物和癌化學(xué)預(yù)防藥物的篩選，結(jié)果表明組分6、7具有選擇性殺傷腫瘤細(xì)胞的活性，組分3、6具有肯定的抗突變效應(yīng)麗組分7抗突變作用顯著。最后，運(yùn)用紅外技術(shù)對(duì)篩選出來(lái)的組分進(jìn)行測(cè)定，建立紅外光譜指紋圖譜，為探索纖維堆囊菌予實(shí)體提取物的生物活性作用機(jī)理提供依據(jù)。綜上所述，本論文工作為粘細(xì)菌子實(shí)體進(jìn)一步的藥物研發(fā)打下了較為堅(jiān)實(shí)的基礎(chǔ)。關(guān)鍵詞粘細(xì)菌；纖維堆囊菌；予實(shí)體；毒理學(xué)分析；生物學(xué)活性；紅外光譜分析。4

簡(jiǎn)介：廈門(mén)大學(xué)博士學(xué)位論文條紋斑竹鯊養(yǎng)殖生物學(xué)、核型和線粒體DNA序列的研究姓名謝仰杰申請(qǐng)學(xué)位級(jí)別博士專(zhuān)業(yè)海洋生物學(xué)指導(dǎo)教師蘇永全20080701條紋斑竹鯊養(yǎng)殖生物學(xué)、核型和線粒體DNA序列的研究7條紋斑竹鯊的二倍體染色體數(shù)為2N104，在已知染色體核型的軟骨魚(yú)類(lèi)中為二倍體數(shù)最多的種類(lèi)之一，核型公式為2N40M26SM8ST30T，染色體總臂數(shù)NF170，其中第3L對(duì)染色體帶有隨體。8對(duì)采自廈門(mén)和湛江海域的條紋斑竹鯊的線粒體DNA控制區(qū)和CYT6基因進(jìn)行PCR擴(kuò)增和測(cè)序，分析了線粒體DNA控制區(qū)、CYT6基因和TRNA‰基因全序列GENBANK序列號(hào)EU363740～EU363752。結(jié)果表明，TRN礦?；蜷L(zhǎng)度為69BP，控制區(qū)長(zhǎng)度為1094～1096BP，CYT6基因全序列長(zhǎng)度為1146BP。TRNA№基因非常保守，未發(fā)現(xiàn)堿基變異。所測(cè)30個(gè)個(gè)體中，控制區(qū)只有4個(gè)多態(tài)位點(diǎn)，5種不同的單倍型；CYT6基因全序列共有10個(gè)多態(tài)性位點(diǎn)，7種單倍型。CYT6基因的1146個(gè)堿基編碼381個(gè)氨基酸，氨基酸鏈共有3個(gè)變異位點(diǎn)，4種單倍型，廈門(mén)群體具有4種單倍型，湛江群體只有1種單倍型。綜合CYT6基因和控制區(qū)序列，2個(gè)條紋斑竹鯊群體共有LO種單倍型，其中廈門(mén)群體有9種單倍型，湛江群體僅4種單倍型。研究結(jié)果表明條紋斑竹鯊在控制區(qū)和CYT6基因序列上的遺傳多樣性均較低，廈門(mén)群體的遺傳多樣性水平高于湛江群體?！P(guān)鍵詞條紋斑竹鯊；養(yǎng)殖生物學(xué)；胚胎發(fā)育；核型；線粒體DNA

簡(jiǎn)介：蘇州大學(xué)學(xué)位論文使用授權(quán)聲明本人完全了解蘇州大學(xué)關(guān)于收集、保存和使用學(xué)位論文的規(guī)定，即學(xué)位論文著作權(quán)歸屬蘇州大學(xué)。本學(xué)位論文電子文檔的內(nèi)容和紙質(zhì)論文的內(nèi)容相一致。蘇州大學(xué)有權(quán)向國(guó)家圖書(shū)館、中國(guó)社科院文獻(xiàn)信息情報(bào)中心、中國(guó)科學(xué)技術(shù)信息研究所含萬(wàn)方數(shù)據(jù)電子出版社、中國(guó)學(xué)術(shù)期刊光盤(pán)版電子雜志社送交本學(xué)位論文的復(fù)印件和電子文檔，允許論文被查閱和借閱，可以采用影印、縮印或其他復(fù)制手段保存和匯編學(xué)位論文，可以將學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫(kù)進(jìn)行檢索。涉密論文口本學(xué)位論文屬在年一月解密后適用本規(guī)定。非涉密論文留論文作者簽名也魚(yú)呸日期雌必導(dǎo)師簽名，瓣日期趔天然鈣網(wǎng)蛋白和重組鈣網(wǎng)蛋白免疫生物學(xué)活性的比較研究目錄目錄中文摘要5ABSTRACTJ7第一章前言9第二章實(shí)驗(yàn)材料與方法1121實(shí)驗(yàn)材料12211試劑與培養(yǎng)基122，12菌株與細(xì)胞株12213抗體12214ELISA檢測(cè)試劑盒12215實(shí)驗(yàn)動(dòng)物及細(xì)胞12216儀器設(shè)備與耗材1322試驗(yàn)方法13221NCRT純化方法13222RCRT純化方法15223RCRT各個(gè)組分分離16224SDSPAGE及NATIVEPAGE17225WESTERNBLOT實(shí)驗(yàn)過(guò)程17226考馬斯亮藍(lán)法測(cè)定蛋白濃度18227MTT法檢測(cè)細(xì)胞增殖19228小鼠腹腔巨噬細(xì)胞的制備19229蛋白刺激細(xì)胞實(shí)驗(yàn)192210NCRT與LPS之間共刺激實(shí)驗(yàn)202211ELISA法檢測(cè)細(xì)胞因子211

簡(jiǎn)介：華南理工大學(xué)碩士學(xué)位論文福建、天津淡水蛭弧菌的分離及生物學(xué)特性研究姓名郭慶東申請(qǐng)學(xué)位級(jí)別碩士專(zhuān)業(yè)食品科學(xué)指導(dǎo)教師蔡俊鵬20090501高培養(yǎng)液中蛭弧菌的最高培養(yǎng)濃度，初步設(shè)想制備更高濃度蛭弧菌培養(yǎng)液的町行方法是在培養(yǎng)過(guò)程中，不斷補(bǔ)加新鮮宿主至培養(yǎng)液，使培養(yǎng)液中的宿主濃度維持在2X1010EFU／ML左右，然后經(jīng)過(guò)長(zhǎng)時(shí)問(wèn)培養(yǎng)制得。關(guān)鍵詞蛭弧菌；致病菌；裂解能力；生長(zhǎng)繁殖能力IL

簡(jiǎn)介：Y77339學(xué)校代碼10264研究生’鏟號(hào)M02010003上海水產(chǎn)大學(xué)碩士學(xué)位論文題目長(zhǎng)江口中華鱘幼魚(yú)的生物學(xué)特性及其保護(hù)BIOLOGYANDCONSERVATIONOFJUVENILECHINESE英文題目STURGEON，4CIPENSERSINEMIS，INⅡRYANGTZERIVERESTUARY專(zhuān)業(yè)水產(chǎn)養(yǎng)殖研究方向姓名魚(yú)類(lèi)生態(tài)學(xué)毛翠風(fēng)指導(dǎo)教師莊甲研究員、章龍珍研究員二OO五年五月上海水產(chǎn)大學(xué)2005屑碩士學(xué)位論文較快。上述結(jié)果說(shuō)明7月份野生樣本的豐滿(mǎn)度大于室內(nèi)搶救養(yǎng)殖的樣本，其原因可能有三，一是搶救的樣本因?yàn)槭軅率贵w質(zhì)弱，在恢復(fù)過(guò)程中影響生長(zhǎng)發(fā)育；二是室內(nèi)養(yǎng)殖的環(huán)境條件不如自然環(huán)境條件；三是室內(nèi)養(yǎng)殖的餌料條件不如室外。89月份，野生樣本的豐滿(mǎn)度小于室內(nèi)搶救養(yǎng)殖的樣本，其原因可能有二，～是中華鱘幼魚(yú)適應(yīng)了人工投入足夠的生物餌料一一水蚯蚓二是養(yǎng)殖水體體積小，幼魚(yú)無(wú)需逃避敵害或?qū)ふ沂澄?，運(yùn)動(dòng)量小，攝食多，大部分食物能量轉(zhuǎn)化成脂肪貯城，因此生長(zhǎng)速率較快。本文采用性狀比值來(lái)分析中華鱘幼魚(yú)的形態(tài)變化，在可量性狀中，中華鱘幼魚(yú)的體長(zhǎng)比體高，頭長(zhǎng)比眼間距，頭長(zhǎng)比眼后頭長(zhǎng)，吻長(zhǎng)比眼后頭長(zhǎng)，尾柄長(zhǎng)比尾柄高等比例性狀均由大變小，說(shuō)明其體高、眼間距、眼后頭長(zhǎng)及尾柄高的相對(duì)增長(zhǎng)速度較快，也顯示出豐滿(mǎn)度的提高。中華鱘幼魚(yú)的頭長(zhǎng)比吻長(zhǎng)，頭長(zhǎng)比眼徑，頭長(zhǎng)比吻至須距，頭長(zhǎng)比吻至口距等比例性狀均由小變大，說(shuō)明中華鱘幼魚(yú)的吻長(zhǎng)、眼徑、吻至須距、吻至口距等的相對(duì)增長(zhǎng)速度較慢。在可數(shù)性狀中，只有鰓耙數(shù)繼續(xù)增加14個(gè)。上述形態(tài)變化說(shuō)明中華鱘幼魚(yú)在長(zhǎng)江口逗留期間，其形態(tài)發(fā)育還在進(jìn)一步發(fā)生變化和完善之中。2長(zhǎng)江口中華鱘幼魚(yú)的食性對(duì)長(zhǎng)江口中華鱘幼魚(yú)的食性進(jìn)行了分析，結(jié)果表明中華鱘幼魚(yú)是以肉食性為主的雜食性魚(yú)類(lèi)，食物中含有魚(yú)、蝦、蟹、貝、水生寡毛類(lèi)、端足類(lèi)、枝角類(lèi)、水生昆蟲(chóng)、植物有機(jī)碎屑和泥沙。從食物的出現(xiàn)頻率分析，5月份，胃內(nèi)含物中鉤蝦的出現(xiàn)頻率最高889％，其次是矛尾緞虎魚(yú)778％，海地瓜333％。6月份，矛尾嘏虎魚(yú)的出現(xiàn)頻率最高86O％，其次是窄額絨螯蟹、青蛤稚貝和鉤蝦，分別為456％，439％，404％。7月份，矛尾緞虎魚(yú)的出現(xiàn)頻率最高833％，其次是白蝦、窄額絨螯蟹和青蛤稚貝，分別占250％，167％，167％。其它種類(lèi)比例相對(duì)較低。從食物的重量百分比分析，57月份，胃內(nèi)含物中矛尾綴虎魚(yú)都占有絕對(duì)重量，分別為9078％，7573％年N5884％。其它食物種類(lèi)中，5月份，窄額絨螫蟹的重量百分比占3，01％，鉤蝦占273％。6月份，窄額絨螫蟹占228％，鉤蝦占194％，IT

簡(jiǎn)介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文嗜熱鏈球菌超濃縮培養(yǎng)及生物學(xué)變化規(guī)律姓名袁肖寒申請(qǐng)學(xué)位級(jí)別碩士專(zhuān)業(yè)基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師高學(xué)軍20050501東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)碩1嶼。位論文SUPERCONCENTRATEDINCUBATIONANDBIOLOGICALCHANGESOFSTREPTOCCUSTHERMOPHILUSABSTRACTINORDERTODEVELOPASUPERCONCENTRATEDINCUBATIONMETHODOFSTREPTOCOCCUSTHERMOPHILUS晦0，THERESEARCHWASCONDUCTEDTOSCREENTHEMAINNUTRITIVESUBSTANCESFIRSTLYONTHEBASISOFBASICMEDIUM，ANDTHENTOSCREENTHEMULTIPLICATIONFACTORSMFS，ASARESULTTHEINGREDIENTSOFSUPERCONCENTRATEDSUBSTRATUMWEREDETERMINEDTHEMETABOLICREGULARITIESOFSTREPTOCOCCUSTHERMOPHILUSONTHENUTRITIVESUBSTANCESOFSACCHARINE，PROTEINANDFATINTHECULTUREMEDIUMANDTHE北ANGESOFULTRASTRUCTUREANDTHEAPOPTOSISDURINGSUPERCONCENTRATEDINCUBATIONWERESRUDI列BYAPPLYINGTHEMETHODOFCENTRIFUGESEMICONTINUOUSPROCESSANDBYTHESUPERMICROSCOPE，TUNELSTAININGANDAGAROSEGELELECTROPHORESISAGE，RESPECTIVELY，ANDTHERESEARCHPROVIDEDTHEFOUNDATIONFORTHESUPERCONCENTRATEDINCUBATIONTECHNIQUEOFSTREPTOCOCCUSTHERMOPHITUSINTHEPAPER，THEENRICHMENTCULTUREFORMULAOFSTREPTOCOCCZA“THERTNOPHITUSANDITSCULTURALCONDITIONWEREDETERMINEDTHECULTUREMEDIUMCONTAINEDTHEFOLLOWINGINGREDIENTS15％LACTOSE025％GLUCOSE，1，5％SOYPEPTONE，1O％LACTOALBUMINHYDROLYSATE，15％YEASTEXTRACTPOWDER35％SALINESOLUTION，015％VITAMINC，01％／YSLNE，005％GLUTAMATE，O05％ARGININE，5％MALTWORTAND5％TOMATOEXTRACTTHEENRICHMENTPROCEDUREWASFOLLOWEDBYSEMICONTINUOUSAPPROACHANDTHEBACTERIACOUNTCOULDREACHUPTO10321010CFU／MLBYTHECULTUREMETHODOFSECONDARYCENTRIFUGATION，ABOUT10TIMESHIGHERTHANTHEBASALCULTUREMETHOD，ANDITWASPROVEDTOBEAVERYEFFECTIVESUPERCONCENTRATEDINCUBATIONMETHODFORSTREPTOCOCCUSTHERMOPHILUSINCONCLUSION，THELAGPHASEOFSTREPTOCOCCUSTHERMOPHILUSWASSHORTENINTHEEUTROPHYCULTUREMEDIUM，ANDTHETIMEACHIEVINGTHECULMINATEDPOINTOFLOGARITHMICPHASEWASSHORTEDALSO，ATTHESAMETIME，THESTATIONARYPHASELENGTHENEDAFTERSECONDARYCULTURE，STREPTOCOCCUSTHERMOPHIIUSREACHEDDIRECTLYTHELOGPHASEHARDLYTHROUGHTHELAGPHASE，ANDTHENQUICKLYREACHEDTHEDECLINEPHASETHEREWEREMORERELATIONSOFTHEMETABOLICREGALITIESOFTHETHREEKINDSOFNUTRITIVESUBSTANCESWITHTHESUPERCONCENTRATEDINCUBATIONPROCESSOFSTREPTOCOCCUSTHERMOPHILUSTHEMORETHENUMBEROFTHEBACTERIA，THEQUICKERTHEMETABOLING，WHILETHEFEWERTHESLOWERTHEMETABOLISMMOREOVERTHEREAPPARENTLYEXISTEDMORPHOLOGICCHANGESINTHISCOURSE，ANDTHEREMAYBEEXISTEDTHEAPOPTOSIS，WHICHCORRELATEDWITHBACTERIAPROPAGATIONANDACIDPRODUCINGWITHTHECULTURETIMEPOSTPONED，THENUTRITIVESUBSTANCESINTHECULTUREMEDIUMLACKEDALSO，ANDTHEMORPHOLOGICCHANGEANDAPOPTOSISAPPEAREDMOREOBVIOUSLY【I

簡(jiǎn)介：中國(guó)協(xié)和醫(yī)科大學(xué)博|_學(xué)位論文論文題目人硒蛋白K的硒代半胱氨酸參入機(jī)制及生物學(xué)功能研究研究生陸彩玲導(dǎo)師強(qiáng)伯勤教授袁建剛教授彭小忠副教授學(xué)科專(zhuān)業(yè)生物化學(xué)與分子生物學(xué)研究方向基因功能及表達(dá)調(diào)控入學(xué)時(shí)間2002年9月所在院所中國(guó)協(xié)和醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院中國(guó)醫(yī)學(xué)科學(xué)院基礎(chǔ)醫(yī)學(xué)研究所2005年6月北京中國(guó)協(xié)和醫(yī)科大學(xué)博士學(xué)位論文ABSTRACTSELENIUMISANESSENTIALTRACEELEMENTINMANYLIFEFORMSTHEBIOLOGICALROLESASCRIBEDTOSELEILIUM，INCLUDING血EPREVENTIONOFCANCERAILDCARDIOVASCULARDISEASE，AREMEDIATEDINMOSTCASEST圭1ROUGHTLEEXPRESSIONOFOVER20MAMMALIANSELENOPROTEINSSELENIUMISPRESENTINNATURALLYOCCLLRRLNGSELENOPROTEINSBYCOTRA皿SLATIONALLYINSERTINGINTOPROTCINASSELENOCYSTEINESEC，THE21“ANLINOACIDINTHEGENETICCODE，INRESPONSETOTHEUGACODON，WHICHISCONVENTIONALLYRECOGNIZEDASA臼肌SLATIONALTERNLINALSIGNALINEUKARYOTES，SELENOCYSTEINEINCORPORATIONIMOSELENOPROTEINSREQUIRESTLLEINTERACTIONBETWEENSELENOCYSTEINEINSERTIONSEQUENCESECISELEMENTRESIDINGINTHE3’U11TRANSLATEDREGION3’UTROFSELENOPROTEINMRNASA11DSECISBINDINGPROTEINSBP2，CONTAININGRNABINDINGDOMAINANDRIBOSOMEBINDILLGDOMINRECENTLYMOREANDMOREATTENTIONISPAIDTOMEBI0109ICALNMCTIONANDSELENOCYSTEINEINCORPOMTIONMACHINERYOFSELENOPROTCINSMANYSELENOPROTEINSIDCNDMEDANDCHARACTERIZED，SUCHASNLEGLU詛MIONEPEROXIDASEF抽1ILYAILDTHCMIOREDOXINREDUCTASE加NILY，PERFB黝A(yù)NTIOXIDAMORCELLULARREDOX缸NCTIONWITHSELENOCYSTEINEASACONSTITUEMOFTHEACTIVESITEAMIOXIDANTACTIVITYHASALSOBEENPMPOSEDFORSELPA11DSELWHOWEVERTHEMLESMANYNOVELSELENOPROTEINSPLAYINCELLULARFHNCTIONSANDDISEASEDEVELOPMENTARESTILLUNKNOWNHUINAILSELCNOPROTEINKSELKCOM兩SEDOF94鋤沁ACIDSISANEWMEMBEROFSELENOPROTEINF抽ILYINTHISSTUDY’WEFOCUSONTHESELENOCYSTEINEINSERTIONMECHANISMANDBIOLOGICALACTIONOFSELK，USING75SEINCORPOR砒IONASSAY，WESHOWTHATSELENIMINCO耳，ORATIONINTOSELKISDEPENDENTONME3’UN訂AILSLATEDREGIONOFSELKMRNASUBSEQUENTLYWECLONCDSBP2CDNA矗OMH眥ANTESTISCDNALIBRARYANDOBTAINEDTHERECOMBIN姐TPROTEINSBP2／512COMAININGCTE肌IMLPANOFSBP2FROMRESIDUE343TOTHECTENNINUSUTMZINGTHEPROKARYOTICEXPRESSIONSYSTEMELECTROPHORETICMOBILITYSHIFTASSAYDEMONSTRATESTHATSELKSECISRNALOCATEDIN3’UTRIMERACTSWITHCTERMINALPARTOFSBP22

簡(jiǎn)介：1094055分類(lèi)爭(zhēng)Q96授予學(xué)位單位代碼J10434研究生學(xué)號(hào)20040222山柬裳薯大學(xué)碩士學(xué)位論文大猿葉蟲(chóng)不同地理種群的生態(tài)適應(yīng)性和山東種群生物學(xué)特性的研究研究生董宇奎學(xué)科專(zhuān)業(yè)農(nóng)業(yè)昆蟲(chóng)與害蟲(chóng)防治研究方向昆蟲(chóng)生態(tài)與害蟲(chóng)綜合治理學(xué)院植物保護(hù)指導(dǎo)教師李照會(huì)教授2007年6月18日Ⅲ陸MⅫY啷隅剮GO．≯霉一胤脅糍一霉MC噸始M訓(xùn)耐．1寶勰一N唧山東農(nóng)業(yè)大學(xué)碩士學(xué)位論文中文摘要大猿葉蟲(chóng)COLAPHELLUSBOWRINGIBALY屬鞘翅目COLEPTERA,葉甲科CHRYSOMELIDAE，無(wú)緣時(shí)甲屬COLAPHELLUS，是我國(guó)十字花科蔬菜上的一種重要食葉害蟲(chóng)。本文以其為試驗(yàn)材料，闡述了大猿葉蟲(chóng)山東種群的生物學(xué)特性，系統(tǒng)地對(duì)不同地理種群大猿葉蟲(chóng)的生態(tài)適應(yīng)性進(jìn)行了研究，包括不同的光周期和溫度對(duì)大猿葉蟲(chóng)不同地理種群滯育誘導(dǎo)的作用、不同地理種群大猿葉蟲(chóng)抗寒能力和低溫生存能力，以及光照和溫度對(duì)滯育后種群抗寒性的影響。主要結(jié)果如下1光周期和溫度的滯育誘導(dǎo)對(duì)于江西種群，當(dāng)光周期的暗期長(zhǎng)度為12～24H時(shí)，有效地防止滯育的發(fā)生；而當(dāng)暗期長(zhǎng)度短于12H則誘導(dǎo)不同程度的滯育，4～8H的暗期長(zhǎng)度明顯誘導(dǎo)了較高的滯育率。黑龍江種群和山東種群不存在光周期反應(yīng)。溫度本身能夠誘導(dǎo)滯育的發(fā)生。當(dāng)溫度低于20℃時(shí)，所有的個(gè)體均迸入滯育；隨著溫度的逐漸上升，三種群的滯育比例逐步降低。28℃時(shí)，江西和山東種群的滯育率分別為22％和92％，而黑龍江種群的所有個(gè)體仍處于滯育狀態(tài)當(dāng)溫度達(dá)30℃，江西和山東種群滯育率分別降至5％和78％時(shí)，黑龍江種群滯育率降為94％，出現(xiàn)非滯育個(gè)體。2大猿葉蟲(chóng)耐寒性研究隨著緯度的升高，大猿葉蟲(chóng)耐寒性逐漸增強(qiáng)。黑龍江、山東和江西三種群過(guò)冷卻點(diǎn)均于一月份達(dá)到最低值，分別為23．7“C、22．3℃和20．7℃，種群間差異顯著。試驗(yàn)結(jié)果表明滯育與非滯育成蟲(chóng)的低溫生存能力存在一定的差異一20℃時(shí)，三種群滯育成蟲(chóng)的半致死時(shí)間分別為0．99H、0．75H和O．64H，而非滯育成蟲(chóng)的半致死時(shí)間相對(duì)較短，分別為0．50H、0．34H和0．29H，滯育成蟲(chóng)的耐寒能力明顯強(qiáng)于非滯育成蟲(chóng)，北方種群耐受低溫的能力明顯強(qiáng)于南方種群。其它低溫下的情況也顯示了同樣的結(jié)果。各種群的過(guò)冷卻點(diǎn)都低于當(dāng)?shù)馗髟缕骄鶞囟?，越冬成蟲(chóng)可以保持較高的越冬存活率。4“C低溫馴化LOD后，大猿葉蟲(chóng)黑龍江種群成蟲(chóng)的耐寒能力無(wú)變化，