簡(jiǎn)介：中國(guó)替茗舛學(xué)面憲院學(xué)位論文學(xué)位論文作者指導(dǎo)教師姓名指導(dǎo)小組成員申請(qǐng)學(xué)位級(jí)別專業(yè)名稱研究方向論文答辯日期何天培孫振元研究員李樹(shù)華教授碩士風(fēng)景園林園林植物應(yīng)用2013年6月獨(dú)創(chuàng)性聲明本人聲明所呈交的學(xué)位論文是我個(gè)人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。盡我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人已經(jīng)發(fā)表或撰寫(xiě)過(guò)的研究成果，也不包含為獲得本研究生培養(yǎng)單位或其它教育機(jī)構(gòu)的學(xué)位或證書(shū)而使用過(guò)的材料。與我一同工作的同志對(duì)本研究所做的任何貢獻(xiàn)均已在論文中作了明確的說(shuō)明并表示謝意。學(xué)位論文作者簽名局巧么謅日期矽哆年汐么月才日學(xué)位論文版權(quán)使用授權(quán)書(shū)本學(xué)位論文作者完全了解中國(guó)林業(yè)科學(xué)研究院有關(guān)保留、使用學(xué)位論文的規(guī)定，中國(guó)林業(yè)科學(xué)研究院有權(quán)保留并向國(guó)家有關(guān)部門(mén)或機(jī)構(gòu)送交論文的復(fù)印件和磁盤(pán)，允許論文被查閱和借閱。本人授權(quán)中國(guó)林業(yè)科學(xué)研究院可以將學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫(kù)進(jìn)行檢索，可以采用影印、縮印或掃描等復(fù)制手段保存、匯編學(xué)位論文。保密的學(xué)位論文在解密后適用本授權(quán)書(shū)學(xué)位論文作者簽名11又侈勿導(dǎo)師簽名加J；年D占月坷ET。／一及少”年妒歹月學(xué)位論文作者畢業(yè)聯(lián)系方式工作單位聯(lián)系電話電子郵件通訊地址、郵編

簡(jiǎn)介：內(nèi)蒙古農(nóng)業(yè)大學(xué)研究生學(xué)位論文獨(dú)創(chuàng)聲明本人申明所呈交的學(xué)位論文是我本人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。據(jù)我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包括其他人已經(jīng)發(fā)表或撰寫(xiě)過(guò)的研究成果，也不包括為獲得我?；蚱渌逃龣C(jī)構(gòu)的學(xué)位或證書(shū)而使用過(guò)的材料，與我一同工作的同志對(duì)本研究所做的任何貢獻(xiàn)均已在論文中作了明確的說(shuō)明并表示謝意。申請(qǐng)學(xué)位論文與資料若有不實(shí)之處，本人承擔(dān)一切相關(guān)責(zé)任。論文作者簽名量董2亞日期211壘塹F蘭內(nèi)蒙古農(nóng)業(yè)大學(xué)研究生學(xué)位論文版權(quán)使用授權(quán)書(shū)本人完全了解內(nèi)蒙古農(nóng)業(yè)大學(xué)有關(guān)保護(hù)知識(shí)產(chǎn)權(quán)的規(guī)定，即研究生在攻讀學(xué)位期間論文工作的知識(shí)產(chǎn)權(quán)單位屬內(nèi)蒙古農(nóng)業(yè)大學(xué)。本人保證畢業(yè)離校后，發(fā)表論文或使用論文工作成果時(shí)署名單位為內(nèi)蒙古農(nóng)業(yè)大學(xué)，且導(dǎo)師為通訊作者，通訊作者單位亦署名為內(nèi)蒙古農(nóng)業(yè)大學(xué)。學(xué)校有權(quán)保留并向國(guó)家有關(guān)部門(mén)或機(jī)構(gòu)送交論文的復(fù)印件和電子文檔，允許論文被查閱和借閱。學(xué)校可以公布學(xué)位論文的全部或部分內(nèi)容保密內(nèi)容除外，采用影印、縮印或其他手段保存論文。論文作者簽名幽指導(dǎo)教師簽名日期LIFEHISTORYANDOCCURRENCECHARACTERISTICSOFGALERUCAJOANNISININNERMONGOLIAGRASSLANDABSTRACTGALERUCADAURICAJOANNISISANIMPORTANTLEAFEATINGPESTTHATHASRECENTLYBEENOUTBROKENONALLIUMSPP．INTHELILACEAEFAMILYINTHEINNERMONGOLIAGRASSLANDREGION．ASTHECLIMATEANDECOLOGICALCONDITIONSHAVECHANGED，THEDAMAGEOFGDAURICAHASBEENGRADUALLYSERIOUS．THEREFORE，ITISOFGREATSIGNIFICANCETORESEARCHONTHELIFECYCLEANDOCCURRENCEOFGDAURICA．ITHELPSUSTOKNOWTHEDEVELOPMENTMECHANISMANDINCREASETHEPESTCONTROLEFFECT．INORDERTOCLARIFYTHEOCCURRENCEANDBIOLOGICALCHARACTERISTICSOFGDAURICAANDTOPROVIDESCIENTIFICBASISFORDEVELOPINGINTEGRATEDMANAGEMENT，THEPOPULATIONDYNAMICS，SPATIALDISTRIBUTIONPAAEMOCCURRENCEANDDEVELOPMENTPERIODOFGDAURICAWERESTUDIEDINGRASSLANDSANDINLABORATORYCONDITIONS．THEMAINRESULTSWERESUMMARIZEDASFOLLOWS1．GDAURICAOCCURREDONEGENERATIONEACHYEARININNERMONGOLIA．THEFEMALEDEPOSITEDTHEIREGGSINTOCOWDUNGORSTONEFOROVERWINTER．OVERWINTEREDEGGSHATCHEDINTHEMIDDLEORLATEAPRILINNEXTYEAR．THELARVAEHAVETHREEDEVELOPMENTALSTAGES，F(xiàn)EEDINGBYTHESTEMSANDLEAVESOFTHEGENUSALLIUM偽ILIUMMONGOLIUMREGEL、AILIUMPOLYRHIZUMTURCZEXREGEL、WILDCHINESECHIVESTOCOMPLETETHEIRDEVELOPMENT．LARVAOCCURRENCEPEAKHAPPENEDINCLIMAXINLATEMAY,SOMEMATURELARVAESTARTEDTOPUPATE，ANDITISAPEAKDAMAGEPERIOD．ADULTSSTARTEDTOEMERGEINEARLYJUNE，F(xiàn)EDINSOMEPERIODANDSTARTEDTOOVERSUMMERINLATE．JUNE．THEADULTSHIDINTHECOWDUNGORBUSHES，ALMOSTDIDN’TFEEDTHROUGHTHESUMMER,ANDSTARTEDTOMATEINMIDDLEAUGUSTORLASTAUGUSTANDLAIDEGGSINEARLYSEPTEMBER．2．THESPATIALDISTRIBUTIONPATTERNWASANALYZEDBYTWOREGRESSIONMODELSANDNINEINDICESOFAGGREGATION．THERESULTSINDICATEDTHATGDAURICAPOPULATIONISANAGGREGATEDDISTRIBUTIONINGRASSLANDS．3．WEINVESTIGATEDTHEEFFECTSOF13COMMONFORAGEPLANTSONTHELARVALDEVELOPMENT，SURVIVALANDFEEDINGAMOUNTINT11ELAB．THERESULTSSHOWEDTHATTHELARVAEWASASPECIALISTHERBIVORE，MAINLYFORAGINGTHEGENUSALLIUMF．E．A．MONGOLIUMREGEL，A，RAMOSUML．ANDA．POTYRHIZUMTURCZ．EXREGEL，ANDTHEOTHERTESTPLANTSWERESELDOMEATEN．THEABOVEALLIUMPLANTSHADSIGNIFICANTEFFECTSONTHELARVALANDNYMPHALDEVELOPMENTDURATIONSANDNOSIGNIFICANTEFFECTONTHELARVALSURVIVALRATES．WHENFEDONA．MONGOLIUM，A．RAMOSUMANDA．POLYRHIZUM，THELARVALANDNYMPHALDEVELOPMENTDURATIONSWERE24．27D，27。87D，

簡(jiǎn)介：ATHESISDISSERTATIONSUBMITTEDTOZHENGZHOUUNIVERSITYFORTHEDEGREEOFMASTERPROMOTERS，CLUSTERINGCHARACTERISTICSANDEVOLUTIONARYHISTORYANALYSISINMAIZEBYPENGYANGZHAOSUPERVISORPROFZENGYUANTIANBIOTECHNOLOGYCOLLEGEOFLIFESCIENCEMARCH2014摘要摘要MICRORNA簡(jiǎn)稱MIRNA，是一類在進(jìn)化中高度保守的小分子非編碼RNA，在真核生物的基因表達(dá)調(diào)控方面發(fā)揮重要作用。MIRNA的成熟序列與AGO蛋白有選擇性結(jié)合形成RISC復(fù)合體RNAINDUCEDSILENCINGCOMPLEX，與相應(yīng)靶基因的MRNA結(jié)合使其表達(dá)受到抑制或降解。MIRNA優(yōu)先結(jié)合的靶標(biāo)涉及的調(diào)節(jié)功能廣泛，集中于生長(zhǎng)發(fā)育、信號(hào)轉(zhuǎn)導(dǎo)以及脅迫相應(yīng)。本文研究了玉米B73REFGENV2參考染色體組中161個(gè)MIRNA的164個(gè)MIRNA基因位點(diǎn)，其中最大的一個(gè)家族是MIRL69共有17個(gè)成員，MIRL62、MIR408、M1R482只被檢測(cè)到1個(gè)成員存在于該染色體中，為成員數(shù)最少的家族。真核生物細(xì)胞中沒(méi)有操縱子，在基因表達(dá)的調(diào)控過(guò)程中順式作用元件結(jié)合相應(yīng)的反式作用因子對(duì)RNA聚合酶的轉(zhuǎn)錄具有調(diào)控作用，這些CARECISACTINGREGULATORYELEMENT在基因序列側(cè)面存在與相應(yīng)的基因往往具有一定的距離，能夠與特定的反式作用因子相互作用調(diào)控真核生物基因表達(dá)的時(shí)間與空間特異性?；蚍执噩F(xiàn)象是基因在染色體上的一種存在形式，位于同一個(gè)簇的基因常常是由兩個(gè)或者兩個(gè)以上的家族成員組成，分布在同一個(gè)簇中的基因往往具有協(xié)同調(diào)控作用。全文有以下幾個(gè)重要的結(jié)論L、通過(guò)TSSP預(yù)測(cè)得到的相應(yīng)的啟動(dòng)子，根據(jù)這些TSS位點(diǎn)上游20KB域內(nèi)啟動(dòng)子的數(shù)量將玉米基因組REFGENV2中的161個(gè)MIRNA的164個(gè)基因分為四組，分別為未檢測(cè)到啟動(dòng)子，僅含有一個(gè)啟動(dòng)子，含有兩個(gè)啟動(dòng)子的和含有三個(gè)啟動(dòng)子或者更多。玉米MIRNA順式調(diào)控元件在MIRNA基因TSS上游10KB的區(qū)域內(nèi)容納的轉(zhuǎn)錄因子結(jié)合位點(diǎn)的數(shù)量平均為220左右。部分順式調(diào)控元件在所有的MIRNA基因上游區(qū)域廣泛存在，其中排名前十的是CACTFTPPCAL、CURECORECR、DOFCOREZM、ACGTATERDL、GTGANTGLO、EBOXBNNAPA、MYCCONSENSUSAT、GATABOX、ARRLAT、CAATBOXL，與脅迫相關(guān)的調(diào)控元件有CURECORECR涉及銅離子脅迫和氧脅迫、ACGTANRDL涉及早期脫水脅迫、MYCCONSENSUSAT涉及冷脅迫、ARRLAT涉及脅迫與非共生血紅蛋白相關(guān)。CACTFTPPCAL的數(shù)量最多與磷酸烯醇丙酮酸羧化酶相關(guān)，在C4植物固定C02的過(guò)程中發(fā)揮重要作用。DOFCOREZM與DOF蛋白相關(guān)，在植物的生長(zhǎng)發(fā)育過(guò)程中發(fā)揮重要作用。

簡(jiǎn)介：美洲型及歐洲型美洲型及歐洲型PRRSV多重多重TAQMAN熒光定熒光定量RTPCR鑒別檢測(cè)方法的建立及應(yīng)用鑒別檢測(cè)方法的建立及應(yīng)用官家明二○一四二○一四年六月碩士官家明美洲美洲型及型及歐洲歐洲型PRRSV多重多重TAQMAN熒光熒光定量定量RTPCR鑒別鑒別檢測(cè)檢測(cè)方法方法的建的建立及立及應(yīng)用應(yīng)用2014碩士學(xué)位碩士學(xué)位論文廣西大學(xué)學(xué)位論文廣西大學(xué)學(xué)位論文原創(chuàng)性原創(chuàng)性和使用授權(quán)聲明和使用授權(quán)聲明本人聲明所呈交的論文，是本人在導(dǎo)師的指導(dǎo)下獨(dú)立進(jìn)行研究所取得的研究成果。除已特別加以標(biāo)注和致謝的地方外，論文不包含任何其他個(gè)人或集體已經(jīng)發(fā)表或撰寫(xiě)的研究成果，也不包含本人或他人為獲得廣西大學(xué)或其它單位的學(xué)位而使用過(guò)的材料。與我一同工作的同事對(duì)本論文的研究工作所做的貢獻(xiàn)均已在論文中作了明確說(shuō)明。本人在導(dǎo)師指導(dǎo)下所完成的學(xué)位論文及相關(guān)的職務(wù)作品，知識(shí)產(chǎn)權(quán)歸屬?gòu)V西大學(xué)。本人授權(quán)廣西大學(xué)擁有學(xué)位論文的部分使用權(quán)，即學(xué)校有權(quán)保存并向國(guó)家有關(guān)部門(mén)或機(jī)構(gòu)送交學(xué)位論文的復(fù)印件和電子版，允許論文被查閱和借閱，可以將學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫(kù)進(jìn)行檢索和傳播，可以采用影印、縮印或其它復(fù)制手段保存、匯編學(xué)位論文。本學(xué)位論文屬于□保密，在年解密后適用授權(quán)?！酢滩槐Ｃ堋Ｕ?qǐng)?jiān)谝陨舷鄳?yīng)方框內(nèi)打“√”論文作者簽名日期指導(dǎo)教師簽名日期作者聯(lián)系電話電子郵箱

簡(jiǎn)介：分類號(hào)密級(jí)華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文遞增與倍增C02濃度對(duì)多代褐飛虱生活史及生化物質(zhì)的影響EFFECTOFGRADUALANDDOUBLEINCREASINGC02CONCENTRATIONSONTHELIFEHISTORYANDBIOCHEMICALSUBSTANCESINMULTIPLEGENERATIONSOFNILAPARVATALUGENS研究生莊靜學(xué)號(hào)2011301110226指導(dǎo)教師吳剛副教授指導(dǎo)小組雷朝亮教授專業(yè)農(nóng)業(yè)昆蟲(chóng)與害蟲(chóng)防治獲得學(xué)位名稱農(nóng)學(xué)碩士張宏宇教授王滿困教授牛長(zhǎng)纓教授馬偉華副教授研究方向昆蟲(chóng)化學(xué)生態(tài)學(xué)獲得學(xué)位時(shí)間2014年6月26日華中農(nóng)業(yè)大學(xué)植物科學(xué)技術(shù)學(xué)院二O一四年六月遞增與倍增C02濃度對(duì)多代褐飛虱生命表及生化物質(zhì)的影響目錄華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文I摘要IABSTRACTIII縮略語(yǔ)表V第一章文獻(xiàn)綜述1L大氣C02濃度升高對(duì)植食性昆蟲(chóng)的作用過(guò)程211直接作用212間接作用2121對(duì)寄主植物的影響一2122對(duì)植食性昆蟲(chóng)的作用機(jī)理32刺吸式昆蟲(chóng)對(duì)大氣C02濃度升高的響應(yīng)321生長(zhǎng)發(fā)育和繁殖等生物學(xué)上的響應(yīng)322行為上的響應(yīng)523生理生化上的響應(yīng)63本文的研究?jī)?nèi)容74本文的研究路線9第二章大氣C02濃度升高對(duì)連續(xù)16代褐飛虱生長(zhǎng)發(fā)育和繁殖的影響101引言102材料和方法1L21試驗(yàn)材料11211試驗(yàn)處理1L212水稻品種1L213供試蟲(chóng)源一LL22試驗(yàn)方法一LL221褐飛虱生長(zhǎng)發(fā)育測(cè)定1L222褐飛虱種群特性測(cè)定1223數(shù)據(jù)分析123結(jié)果與分析12

簡(jiǎn)介：廈門(mén)大學(xué)學(xué)位論文原創(chuàng)性聲明本人呈交的學(xué)位論文是本人在導(dǎo)師指導(dǎo)下，獨(dú)立完成的研究成果。本人在論文寫(xiě)作中參考其他個(gè)人或集體己經(jīng)發(fā)表的研究成果，均在文中以適當(dāng)方式明確標(biāo)明，并符合法律規(guī)范和廈門(mén)大學(xué)研究生學(xué)術(shù)活動(dòng)規(guī)范試行。另外，該學(xué)位論文為勉綿動(dòng)物當(dāng)夠誨課題組的研究成果，獲得碧慟坯蒯嬲題組經(jīng)費(fèi)或?qū)嶒?yàn)室的資助，在握鼢7弛勿實(shí)驗(yàn)室完成。請(qǐng)?jiān)谝陨侠ㄌ?hào)內(nèi)填寫(xiě)課題或課題組負(fù)責(zé)人或?qū)嶒?yàn)室名稱，未有此項(xiàng)聲明內(nèi)容的，可以不作特別聲明。為燁年J月聲明人簽名勿移極縐目錄目錄摘要???????????????????????????．IABSTRACT?????????????????????????????????．．ⅡI第一章緒論????????????????????????11．1海綿動(dòng)物的生物學(xué)特性研究??????????????????．11．2海綿動(dòng)物的研究?jī)r(jià)值?????????????????????．21．2．1海綿動(dòng)物骨針特性研究??????????????????21．2．2海綿動(dòng)物活性物質(zhì)????????????????????41．3海綿動(dòng)物生活史的研究概況??????????????????．51．4研究主要目的、意義和內(nèi)容??????????????????．7第二章葉片山海綿的早期胚胎發(fā)育?????????????一92．1引言????????????????????????????．92．2材料和方法?????????????????????????102．2．1主要儀器和試劑????????????????????．102．2．2海綿樣品采集?????????????????????．102．2．3石蠟切片制備?????????????????????．112．2．4HE染色????????????????????????112．3結(jié)果?????????????????????????????????????122．3．1卵細(xì)胞形成??????????????????????．122．3．2精子發(fā)生???????????????????????．．132．3．3胚胎發(fā)育???????????????????????。162．3．4幼體形成???????????????????????．182．3．5繁殖期????????????????????????．202．4討論????????????????????????????22第三章海綿動(dòng)物幼體釋放規(guī)律以及稚體生長(zhǎng)的研究??????263．1引言????????????????????????????263．2材料和方法?????????????????????????263．2．1海綿動(dòng)物幼體釋放實(shí)驗(yàn)?????????????????．26

簡(jiǎn)介：中圖分類號(hào)中圖分類號(hào)J213單位代碼單位代碼10231學(xué)號(hào)號(hào)2013301026美洲紅點(diǎn)鮭和白斑紅點(diǎn)鮭及其雜交子代的生美洲紅點(diǎn)鮭和白斑紅點(diǎn)鮭及其雜交子代的生長(zhǎng)性能和長(zhǎng)性能和遺傳多樣性遺傳多樣性研究研究學(xué)科專業(yè)動(dòng)物學(xué)研究方向脊椎動(dòng)物學(xué)作者姓名郭媛指導(dǎo)教師趙文閣教授王炳謙研究員哈爾濱師范大學(xué)二〇一五年六月ATHESISSUBMITTEDFTHEDEGREEOFMASTERTHEGROWTHPERFMANCEGEICDIVERSITYOFSALVELINUSLEUCOMAENISSALVELINUSFONTINALISTHEIRHYBRIDOFFSPRINGSCIDATEYUANGUOSUPERVISWENGEZHAOPROFESSBINGQIANWANGPROFESSSPECIALITYZOOLOGYDATEOFDEFENCEMAY，2015DEGREECONFERRINGINSTITUTIONHARBINNMALUNIVERSITY

簡(jiǎn)介：大連水產(chǎn)學(xué)院碩士學(xué)位論文利氏才女蟲(chóng)生活史及防治的研究姓名張樹(shù)林申請(qǐng)學(xué)位級(jí)別碩士專業(yè)水產(chǎn)養(yǎng)殖指導(dǎo)教師李永函周一兵200051利氏才女蟲(chóng)生活史及防治的研究THESTUDYONLIFEHISTORYANDPREVENTIONANDTREATMENTOFPOLFDORAL／GN／WEBSTERZHANGSHULNQDEPTOFAQUCULTUREDALIANFISHERIESCOLLEGE，DALIAN116023ABSTRACTTHISPAPERREPORTSTHEEXISTENCEOFPOLYDORALIGNIWEBSTERINCHINAANDELUCIDATESITSLIFEHISTORYINTHEPONDSROFIFERSREARINGANDLARVAEOFCRABSREARINGINADETAILFORTHEFIRSTTIMETHEEXPERIMENT’SRESULTSHOWSAT163“2EGGSINTHEEGGBAGDEVELOPINTO3SEGMENTPELAGICLARVAEAFTER4DAYSANDLEADAPELAGICLIFEINWATERTHE14SEGMENTPELAGICLARVAEBEGINMETAMORPHOSIS13DAYSAFTERHATCHERY，19DAYSLATERTHEYDEVELOPINTO21SEGMENTDESEENDANDBOT幻MEBENTHIC，AFTER21DASTHEBENTHICLARVAEWITH23SEGMENTCOMPLETEMETAMORPHOSISANDSHOWTHEADULTTUBEBUILDINGHABITTHEEXPCRIT’SRESULTOFTLIE／ZLTG帕LAMAESOFFEETO／1BRACHIONUSPOLICATILIXANDZOEAOFCRABSSHOWSP1IGNILARVAEDORA【HEA“GREATHARMTOTIFEXSANDALSOBADLYENDANGERIANDHOFCLABSTHE95％CONFIDENCEINTERVALOFCAZIBINGS24HRLE50AND48HRLC50TOPLIGNILARVAEBPOLICAFILISANDZOEAOFCRABS8IE只LIGNILARVAE4GL41564皿G幾，385342一34RAG／L；置POLICATILTS2795236L旬309MGN，188524913137MG，L；IZOEAANDO，CRABS，48924487～5333MG／L，393539706MG／L；HZOEAOFCRABS50254593～5497M礬。，43113582Q324MG／LTHISPAPERCONDUCTPRELIMINARYRESEARCHONTHESALINITY’SEFFECTONTHESAVIVALOF只LIGNILARVAE，AECORDI】AGTOALLTHESERESULTSTHEAUTHORPUTFORTHCORRESPONDINGMETHODAOFPREVENTIONANDTREATMENT

簡(jiǎn)介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文美洲擬鰈抗凍蛋白基因植物表達(dá)載體的構(gòu)建姓名李樹(shù)峰申請(qǐng)學(xué)位級(jí)別碩士專業(yè)基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師嚴(yán)云勤200251李樹(shù)峰ABSTRACT2002年5月CONSTRUCTIONOFPLANTEXPRESSIONVECTORHARBORINGPSEUDOPLEURONECTESAMERICANUSANTIFREEZEPROTEINGENEABSTRACTTHEANTIFREEZEPROTEINSHAVEBEENFOUNDINMANYORGANISMS，SUCHASFISHES，PLANTSANDINSECTSANDTHEANTIFREEZEPROTEINSINFISHESARESTUDIEDINDETAILTHEANTIFREEZEPROTEINSHAVEMANYAPPLICATIONSININCREASINGTHEQUANTITYANDQUALITYOFCROPIMPROVINGTHESEEDLINGLIVIABILITY，PROLONGINGTHEGROWTHSEASONOFCROPANDEXTENDINGTHEPLANTINGRANGE，ASWELLASDECREASINGTHESTOREDTEMPERATUREOFCROPTOPROLONGTHESTOREDTIMEMOREOVER，THEREAREMANYIMPORTANTAPPLICATIONSINMEDICINE，F(xiàn)OODSTUFFSCIENCEANDINDUSTRYINOUREXPERIMENT，ALOTOFREGULATORSEQUENCESINCLUDINGTHE35SPROMOTERWITHDOUBLEENHANCERELEMENTSANDTHETERMINATORNOSTHENSEQUENCEANDKOZAKSEQUENCEFORIMPROVINGTHEGENETICSTATEMENTINTHELEVELOFTRANSLATION，WEREAPPENDEDONTHETWOSIDESOFTHEARTIFICALLYPSEUDOPLEURONEETESAMERICANUSANTIFREEZEPROTEINGENEBYASERIESOFPROCESSANDTHEPRODUCTSWEREINKEDINTOPBI】21】TOGETTHEEXPRESSIONVECTORFORTHEANTIFREEZEPROTEINGENEFOLLOWEDBYIMPORTINGITINTOTHECALUUSOFMAIZEBYGENEGUNATLAST，WEDETECTEDTHEEXPRESSIONOFGUSGENELINKEDTOANTIFREEZEPROTEINGENETHERESULTINDICATESTHATGUSGENEISEXPRESSEDSTRONGLYANDINSTANTANEOUSIYINCALUUSOFMAIZEKEYWORDCARRIERDESIGNANTIFREEZEPROTEINREGULATORSEQUENCE

簡(jiǎn)介：中文摘要黃腹?jié)撓壚O蜂OPIUSCARICIVORAEFISCHER是美洲斑潛蠅LIRIOMYZASATIVAEBLANDCHARD幼蟲(chóng)．蛹期的一種重要內(nèi)寄生蜂。本文在實(shí)驗(yàn)室條件下用菜豆PHASEOLUSVULGARISL．上的美洲斑潛蠅幼蟲(chóng)作為寄主，對(duì)該蜂的生物學(xué)、生態(tài)學(xué)特性做了研究。研究結(jié)果如下1黃腹?jié)撓壚O蜂幼蟲(chóng)為6齡，一齡幼蟲(chóng)特化成具顎型，并相互攻擊，以后5齡幼蟲(chóng)為顯頭型，蛹為離蛹，在寄主蛹內(nèi)結(jié)繭化蛹。并詳細(xì)記述了各蟲(chóng)態(tài)的形態(tài)特征。2測(cè)定了17、21、25和33℃下卵、各齡幼蟲(chóng)和蛹的發(fā)育速率，線性日度模型和TLOGISTIC模型均能很好擬合各蟲(chóng)態(tài)的發(fā)育速率和溫度的關(guān)系。／在實(shí)驗(yàn)溫度下，卵、幼蟲(chóng)、L蛹和卵一蛹的發(fā)育歷期變化分別為2．54～7，69、0．5肚2．73、7．24～13，09和14．47～32．43天，發(fā)育起點(diǎn)溫度分別為11．48。C、7．74～12．80℃、1．60℃、8．03℃，有效積溫分別為41．32、10．27～17、89、208．33、303．03日度。蛹在低溫下就可以開(kāi)始發(fā)育，由此可以推斷，在田間，該蜂可能以蛹越冬。在33。C69．8％的蛹休眠。整個(gè)幼期的存活率和溫度的關(guān)系符合雙曲線方程SO．009嚴(yán)O．410T．3．872震0．9583。卵的存活率在89．6％以上，幼蟲(chóng)的存＼活率在81．3％以上，蛹的存活率變化幅度大，25。C時(shí)為95．5％．334C時(shí)只有8．7％。尹一F，F(xiàn)3成蟲(chóng)主要在白天羽化，羽化高峰期在800～1000，且多數(shù)9L％當(dāng)日交尾，羽化出的成蜂性比明顯偏雌性早61．511；在17“C一336C下，提供蜂蜜液時(shí)，雌蜂和雄峰的壽命隨著溫度的升高而縮短，雌蜂的壽命明顯長(zhǎng)于雄峰的壽命，分別從17。C時(shí)8L天和47天降到33。C時(shí)的14天和13天；在25“C下，黃腹?jié)撓壚O蜂在羽化后12小時(shí)體內(nèi)已懷有卵，羽化后2～7日齡達(dá)到懷卵高峰期，在第三天達(dá)最大值，平均每雌懷卵數(shù)為24．1粒，之后，逐漸減少。雌蜂羽化后L2天即可產(chǎn)卵，即使不交尾，該蜂也有產(chǎn)卵能力。在21。C29。C溫度下，產(chǎn)卵高峰期在27日齡，平均產(chǎn)卵量隨溫度變化的方程為Y．O．057X22．728X一20．601R2O．934，而且成蜂取食也能致死寄主。另外，本論文對(duì)雌蜂的寄主搜索、產(chǎn)卵和寄主取食行為做了比較詳細(xì)的描述。Z一等凈生殖率奶在21℃達(dá)到最大114，7，隨溫度升高或降低，‰均減少；內(nèi)頻增長(zhǎng)率‰和周限增長(zhǎng)率九均隨著溫度的升高而上升，兩參數(shù)均在29“C達(dá)到最大值，隨后又逐漸下降。序5℃和29。C的RM值大約是17“C1拘1倍。種群趨勢(shì)指數(shù)和溫度呈拋物線關(guān)系，回歸方程為，一0．904，41．166T．374．320冠20．993．種群增長(zhǎng)趨勢(shì)指數(shù)在25“C最大94．21，而33。CIJ種群趨勢(shì)指數(shù)0．78小于1，說(shuō)明33“C時(shí)黃腹?jié)撓壚O蜂種群將減少。STUDIESONBIOLOGYANDECOLOGYOFOPIUSCARICIVORAEFISCHERHYMENOPTERABRACONIDAE，APARASITEOFLIRIOMYZASATIVAEBLANDCHARDDIPTERAAGROMYZIDAEABSTRACTOPIUSCARICIVORAEFISCHERISAMAJORLARVALPUPALENDOPARASITOIDOFLIRIOMYZASATIVAEBLANDCHARD．THEBIOLOGYANDECOLOGYOFTHISPARASITOIDWERESTUDIEDUNDERLABORATORYCONDITIONBYUSINGL．SATIVAEONTHEBEANPHASEOLUSVULGARISLASTHEHOST．THELALNAEOFO．CARICIVORAEUNDERWENTSIXINSTARS．THE1STINSTARLARVAEWEREMANDIBULATEWHILETHELARVAEOFOTHERFIVEINSTARSWEREHYMENOPTERIFORM．THELSTINSTARLARVAECOULDARACKEACHOTHER．THE6THINSTARLARVAESPUNACOCOONINTHEHOSTPUPAWHEREITPUPATED．THEMORPHOLOGICALCHARACTERSOFTHEPARASITOIDLARVAEOFEACHINSTARSWEREDESCRIBEDJNDETAIL．THEADULTPARASITOIDSEMERGEDBYDAYLIGHTWITHAPEAKAT8001000．ABOUT91％OFTHEADULTSMATEDONTHEEMERGENCEDAY．FEMALEBIASEDADULTSEXRATIOWASFOUNDINLABORATORYPOPULATIONSF旱61．5111．LONGEVITYOFBOTHFEMALEANDMALEADULTSDECREASEDWITHINCREASEOFTEMPERATURESFROM17℃TO33℃WHENHONEYBEESOLUTIONWASPROVIDED．MEANLONGEVITYOFFEMALESWASLONGER8LDAT17℃AND14DAT33℃THANTILATOFMALES47DAT17℃AND13DAT33。C．FEMALEADULTSSTARTEDOVIFLOSITIONON12DAFTEREMERGENCEWITHAPEAKON27DFROM21℃TO29℃．THERELATIONSHIPBETWEENAVERAGEFECUNDITYANDTEMPERATURECOULDBEFITTEDWITHTHEEQUATIONY；．0．057X。2．728X．20．601FR20．934．THEFEMALESWERE如LETOKILLHOSTSBYFEEDING．INADDITION．THEHOSTFEEDING，OVIPOSITIONANDHOSTSEARCHINGBEHAVIORSOFTHEPARASITOIDSWEREDESCRIBED，THEDEVELOPMENTALANDSURVIVALRATESOFTHEEGG．1ALVALANDPUPALSTAGESWEREMEASUREDAT5CONSTANTTEMPERATURESOF17，21，25。29AND33℃．THERESULTSSHOWEDTHATTHEIINEARDEGREEDAYANDTHELOGISTICMODELSCOULDDESCRIBEWELITHERELATIONSHIPBETWEENTHEDEVELOPMENTRATEOFEACHIMMATURESTAGEANDTHEMIDTEMPERATURES．THEDEVELOPMENTDURMIONSOFEGG，LARVA，PUPAANDEGGPUPAVARIEDFROM2．54TO7．69，0．50TO2．73，7．24TO13．09，14．47TO32．43DAYS，RESPECTIVELY．THEDEVELOPMENTTEMPERATURETHRESHOLDFORPUPAWAS1．60℃WITHEFFECTIVEACCUMULATEDTEMPERATURES208．33DAYDEGREE．SUGGESTING血ATTHEPARASITOIDCOULDOVERWINTERBYPUPAINTHEFIELD．NLEDEVELOPMENTTEMPERATURETHRESHOLDSFOREGG．1ARVAANDEGGPUPAWEREL1．48，7．74～12．80AND8．03℃WIMEFFECTIVEACCUMULATEDTEMPERATURES4I．32，10．27～17，89AND303．03DAYDEGREE．RESPECTIVELY．ABOUT69．8PERCENTAGEOFPUPAEUNDERWENTDORMANCYATORABOVE33℃．THERELATIONSHIPBETWEENSURVIVAIRATEANDTEMPERATURECOULDBEFITTEDWITHTHEEQUATIONS一0．009R0．410T3．872F尺O．9583．THESURVIVALRATESOFTHEEGGANDIARVAEWEREMORETHAN89．6％AND81_3％，RESPECTIVELY．PUPALSURVIVALRATEVARIEDGREATLYFROM8．7％AT33℃TOABOVE83．0％FROM17℃TO29℃．THENETREPRODUCTION‰PEAKEDAT21℃ANDDECLINEDBOTHABOVEANDBELOWTHISTEMPERATURE．HOWEVEGBOTHTHEINTRINSICRATEOFINCREASERMANDFINITERATEOFINCREASE¨INCREASEDFROM17。CTO29℃ANDDECREASEDFROM29℃TO33℃．THEINTRINSICRATEOFINCREASERMAT21℃OR25℃NEARLYDOUBLEDCOMPAREDWITHTHATAT174C．THERELATIONSHIPBETWEENTHE

簡(jiǎn)介：湖南農(nóng)業(yè)大學(xué)碩士學(xué)位論文美洲商陸抗病毒蛋白基因在畢赤酵母中的表達(dá)姓名周倩申請(qǐng)學(xué)位級(jí)別碩士專業(yè)植物病理學(xué)指導(dǎo)教師高必達(dá)王錫鋒200361ABSTRACT1LANTVIRUSDISEASEISONEPRIMARYCROPDISEASEITDISTRIBUTESWILDLYANDDOESGREATHARMTOECONOMYOFAGRICULTUREYIELDPEOPLESEARCHAFTERWAYSTOPREVENTIONANDC11REVIRUSDISEASESFORLONGTIMEBUTNOTDEVELOPEFFECTIVEREAGENTYETMANYSTUDIESREVEALEDTHATPOKEWEEDANTIVIRALPROTEINPAPCANCONTROLPLANTVIRUSEFFECTIVELYBUTPAPINNATUREISLIMITEDWHICHCOULDNOTAPPLYCOSMICALLYUSINGBIOREACTERTOPRODUCEPAPISONEEFFICACIOUSWAYTOGETAMASSOFPAESOMEONETRIEDTHISWAYBUTTHEYIELDISLOWFRANCISEXPRESSEDTRUNCATEDMUTANTPOKEWEEDANTIVIRALPROTEINGENEINPICHIAPASTORISTHEYIELDREACHED1012MG／LDRCHENDINGHUINPLANTVIRUSGROUPOFINSTITUTEOFPLANTPROTECTIONOFTHECAASCHINESEACADEMYOFAGRICULTURESCIENCESLSELECTEDPAPMUTSRECOMBINANTSANDWANTEDTOPRODUCEPAPTHROUGHFERMENTATIONTOPREVENTIONANDCUREPLANTVIRUSDISEASEBUTASTOMUTSRECOMBINANTSTHEPERIODSOFFERMENTATIONISTOOLONGANDTHEEFFICIENCYOFUSINGMETHANOLISLOWSOUSINGMUT5RECOMBINANTSTOPRODUCEPAPINFACTORYISNOTFELICITOUSNESSLINEARIZEDTHEEXPRESSIONVECTORPPIC9KPINCLUDINGTHETRUNCATEDMUTANTPOKEWEEDANTIVIRALPROTEINPAPGENEBYRESTRICTIONENDONUCLEASESAL1ANDTRANSFORMEDITELECTRICALLYINTOPICHIAPASTORISGSLL5MUT十RECOMBINANTSWERESELECTEDBYPCRANDHIIGHYIELDMUTRECOMBINANTWASPICKEDOUTBYDOUBLEFILMIMMUNOBLOTTINGTOFINDOUTTHEDIFFERENCEBETWEENMUTANDMUTSRECOMBINANTSWECOMPAREDTHEIREXPRESSIONOFPOKEWEEDANTIVIRALPROTEININTHESAMECONDITIONSSDSPAGERESULTSSHOWEDTHATTHEREWASACLEARTARGETPROTEINBANDINMTITRECOMBINANTSUPERNATANTAFTER48HOURSOFCULTURING，WHILEAFAINTBANDONLYINMUTSRECOMBINANTAFTER72HOURSWESTERNBLOTTINGRESULTSHOWEDTHATTHEREWASNEMARKABLEDIFFERENCEOFYIELDBETWEENMUTANDMUTSRECOMBINANTSAFTER6DAYSINDUCEDANTIVIRUSACTIVITYTESTSREVEALEDTHATCULTURESUPEMATANTSOFMUTANDMUT8RECOMBINANTSCOULDINHIBITTMVINFECTIONWITHHIGHEFFICIENCYINTHESAMECONCENTRATIONANDTHEREWASNOSIGNIFICANTDIFFERENCEBETWEENTHEMDIFFERENTCULTIVATIONCONDITIONSUCHASINDUCEDTIME，METHANOLCONCENTRATIONANDPHOFCULTUREMEDIUMWASSTUDIEDATSHAKEFLASKCULTIVATIONSDSPAGERESULTSSHOWEDTHATASTOMUTRECOMBINANTHIGHESTYIELDWASOBTAINEDAFTER4DAYSINDUCINGANDWITHTHECULTURE

簡(jiǎn)介：福建農(nóng)林大學(xué)碩士學(xué)位論文美洲大蠊誘捕器及誘餌的研究姓名周玉峰申請(qǐng)學(xué)位級(jí)別碩士專業(yè)農(nóng)業(yè)昆蟲(chóng)與害蟲(chóng)防治指導(dǎo)教師吳珍泉20040401THERESEARCHOFTRAPANDHAITTOPERIPLANETAAMERICANAABSTRACTINTHISARTICLE，THEBEHAVIORRESPONSEOFPERIPLANETAAME，LCANATOCOLOR、ROUGHNESSANDBARRIERWASSTUDIEDITWASFOUNDTHAT只AMERICANATENDSTOSETTLEROUGHERSURFACE，BEBLUNTTOTHECHANGEOFCOLOR，F(xiàn)AVORMOVINGABOVEIFTHEANGLEBETWEENTHEBARRIERANDTHEMOVINGDIRECTIONOFROACHESEQUALSTO115。ORLARGER，95％OFTHEROACHESWILLCONTINUETOMOVEFORWARDTOTHESIDEOFTHEOBTUSEANGLETHEANGLEBETWEENBAFFLESANDTHEBOTTOMBRIMOFTHETRAPWASDESIGNED25。THEOBTUSEANGLEIS115。THATLEADSMOSTCOCKROACHESMARCHINGINTOTHETRAPTHELENGTH，WIDTHANDWEIGHTOFPAMERICANAIMAGOWEREMEASUREDTHELENGTHBETWEENTWOPOSTPEDESIS213～264CMWITHTHEAVERAGEOF241CMITSBODYLENGTHIS348～448CMWITHTHEAVERAGEOF385CM，ANDITSWIDTHISL_15～163CMWITHTHEAVERAGEOF148CMTHECOCKROACHTRAPHASFOURSMALLDOORSTHATROACHESCANGETIOBUTNOTOUTONTHEBASISOFCOCKROACHES’BODYLENGTHANDWIDTH，THESEDOORSARE28CMINLENGTHAND2OCMINWIDTHTHECOCKROACHTRAPWASDESIGHEDTHREESHELVESTHATCAMBETAKEAPART，THECENTRALOFBOTTOMLAYERWASUSEDTOPLACEBAITTHATATTRACTSCOCKROACHESCRAWLINGTHROUGHSLOPEOFBOTTOMTOTHEMIDSTLAYERTHEMIDSTLAYERHASSMALLDOORSANDGROOVEWHICHFORMSLOPETHETOPLAYERWASALUCIDITYCOVERITWASFOUNDTHATTHEAPPETITEOFPAMERFCANAIMAGOAEHIEVESTHEMAXIMUMWHENITSDENSITYIS5774PERSTERETHECOCKROACHTRAPLURESROACHESWITHNATURALATTRACTANTSWHICHARECORN，LEGUME，VEGETABLE，ANDOTHERORDINARYFOODINDIANMEAL，POWDERYYEAST，HONEYANDBUTTERWERESELECTEDTOMAKEBAITAORTHOGONALDESIGNWASMADETOABTAINTHEBESTPROPORTIONOFTHEINGREDIENTSOFTHEBAIT，THEOPTIMUMPROPORTIONOFINDIANMEAL，POWDERYYEAST，HONEYANDBUTTERWAS6323THEOPTIMUMMOISTUREIS5325％KEYWORDSPERIPANETAAMERICANATRAPBAIT

簡(jiǎn)介：東北農(nóng)業(yè)大學(xué)博士學(xué)位論文國(guó)內(nèi)外PRRSV美洲型毒株結(jié)構(gòu)基因序列分析及感染豬病理和細(xì)胞免疫變化的研究姓名侯振中申請(qǐng)學(xué)位級(jí)別博士專業(yè)獸醫(yī)外科學(xué)指導(dǎo)教師王林安200361了國(guó)內(nèi)流行的SD毒株和D。毒株毒株均屬于美洲型，與美洲型毒株具有高度的同源性。通過(guò)確定病毒侵害細(xì)胞和組織的種類和部位，為有針對(duì)性診斷指明采樣方向。并通過(guò)監(jiān)測(cè)對(duì)PRRSV感染豬細(xì)胞免疫和體液免疫，了解了PRRSV感染時(shí)豬體免疫功能的變化規(guī)律。這不僅為疫苗研制提供分子生物學(xué)基礎(chǔ)，而且對(duì)建立快速的臨床病理學(xué)診斷和合理的免疫方案以及高效靈敏的診斷方法創(chuàng)造良好條件。關(guān)鍵詞PRRSV；序列分析；FIA；病理組織學(xué)；組織分布CD4；CD8L／。1|T／T。。，IL

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