簡介：揚州大學碩士學位論文美洲斑潛蠅的種群分化研究姓名陸亞娟申請學位級別碩士專業(yè)農(nóng)業(yè)昆蟲與害蟲防治指導教師杜予州20030501陸亞娟美洲斑潛蠅的種群分化研究距離分別為939～100％年U00000036海南儋縣的美洲斑潛蠅種群為第三類。在此三大類地理種群中，海南儋縣美洲斑潛蠅種群與江蘇揚州和浙江杭州的美洲斑潛蠅種群的遺傳相似性和遺傳距離分別為674OOV瘌00000391，與廣東F31，F(xiàn)、廣西南寧和貴州銅仁的美洲斑潛蠅種群遺傳相似性和遺傳距離分別為202～523％和0478一1233；由此可見，該地區(qū)的種群與江蘇揚州和浙江杭州的種群親緣關系近于與其它地理種群之間的親緣關系。3、通過相同軟件對不同寄主和不同地區(qū)美洲斑潛蠅的13TUBULIN基因序列進行比較分析發(fā)現(xiàn)，該基因序列變異不明顯，親緣關系均大于98％。在寄主種群中，寄主為豆科豇豆、茄科番茄、葫蘆科黃瓜和十字花科青菜的美洲斑潛蠅種群之間的遺傳相似性和遺傳距離分別為99995％和O0050011，寄主為錦葵科棉花的美洲斑潛蠅種群與其它寄主種群之間的遺傳相似性和遺傳距離分別為984990％和0011～0016；在地理種群中。海南儋縣的美洲斑潛蠅種群與江蘇揚州和浙江杭州的美洲斑潛蠅種群的遺傳相似性和遺傳距離分別為99099，5％和O005，它與廣東廣州、貴州銅仁和廣西南寧的美洲斑潛蠅種群的遺傳相似性和遺傳距離分別為98499O％和00110016。從以上13TUBULIN基因序列的分析結果可以看出，與RDNAITS序列相比，該基因的保守性較好，不適合于美洲斑潛蠅種下分化的研究。關鍵詞美洲斑潛蠅種下分化RDNAITUBULIN基因序列分析分子系統(tǒng)學

簡介：謹以此論文獻給我的兩位導師、我的父母、哥嫂及丈夫賀艷美洲牡蠣CRASSOSTREAVIRGINICA抗病相關基因標記的篩選及應用學位論文完成日期三三年6目

簡介：美洲型及歐洲型美洲型及歐洲型PRRSV多重多重TAQMAN熒光定熒光定量RTPCR鑒別檢測方法的建立及應用鑒別檢測方法的建立及應用官家明二○一四二○一四年六月碩士官家明美洲美洲型及型及歐洲歐洲型PRRSV多重多重TAQMAN熒光熒光定量定量RTPCR鑒別鑒別檢測檢測方法方法的建的建立及立及應用應用2014碩士學位碩士學位論文廣西大學學位論文廣西大學學位論文原創(chuàng)性原創(chuàng)性和使用授權聲明和使用授權聲明本人聲明所呈交的論文，是本人在導師的指導下獨立進行研究所取得的研究成果。除已特別加以標注和致謝的地方外，論文不包含任何其他個人或集體已經(jīng)發(fā)表或撰寫的研究成果，也不包含本人或他人為獲得廣西大學或其它單位的學位而使用過的材料。與我一同工作的同事對本論文的研究工作所做的貢獻均已在論文中作了明確說明。本人在導師指導下所完成的學位論文及相關的職務作品，知識產(chǎn)權歸屬廣西大學。本人授權廣西大學擁有學位論文的部分使用權，即學校有權保存并向國家有關部門或機構送交學位論文的復印件和電子版，允許論文被查閱和借閱，可以將學位論文的全部或部分內(nèi)容編入有關數(shù)據(jù)庫進行檢索和傳播，可以采用影印、縮印或其它復制手段保存、匯編學位論文。本學位論文屬于□保密，在年解密后適用授權?！酢滩槐Ｃ堋Ｕ堅谝陨舷鄳娇騼?nèi)打“√”論文作者簽名日期指導教師簽名日期作者聯(lián)系電話電子郵箱

簡介：RESEARCHONHISTORY；PHILOSOPHYANDEXPRESSION0FHOUKUANGCHUN’SACADEMICTHOUGHTADISSEIRTATIONSUBMITTEDTOTHEGRADUATESCHOOLOFTHESOUTHWESTUNIVERSITYINPARTIAL如LFILLMENTOFTHEREQUIREMENTSFORTHEDEGREEOFDOCTOROFPHILOSOPHYPHDINTHEC011EGEOFRESOURCESANDENVIRONMENTOFTHESOUTHWESTUNIVERS時2014BYSHENLIJUAⅡSOUTHWESTUNIVERS時CHONGQING，CHINAJUNE2014本文系中央高?；究蒲袠I(yè)務費專項“侯光炯學術思想研究”編號XDJK2013D008的主要研究內(nèi)容，同時系國家水體污染控制與治理科技重大專項“三峽庫區(qū)及上游流域農(nóng)村面源污染控制技術與工程示范課題”編號2012ZX07104、國家科技支撐項目“三峽庫區(qū)小戶型分散農(nóng)業(yè)經(jīng)營區(qū)面源污染防控關鍵技術研究與示范”編號2012BADL58043、國家自然科學基金面上項目“三峽庫區(qū)紫色土坡耕地一桑樹系統(tǒng)農(nóng)業(yè)面源污染控制機理研究”2013和教育部人文社會科學研究項目“高校人文社會科學研究者信息需求規(guī)律及服務模式研究“編號13XJC870001等的基礎理論研究內(nèi)容。

簡介：中圖分類號中圖分類號J213單位代碼單位代碼10231學號號2013301026美洲紅點鮭和白斑紅點鮭及其雜交子代的生美洲紅點鮭和白斑紅點鮭及其雜交子代的生長性能和長性能和遺傳多樣性遺傳多樣性研究研究學科專業(yè)動物學研究方向脊椎動物學作者姓名郭媛指導教師趙文閣教授王炳謙研究員哈爾濱師范大學二〇一五年六月ATHESISSUBMITTEDFTHEDEGREEOFMASTERTHEGROWTHPERFMANCEGEICDIVERSITYOFSALVELINUSLEUCOMAENISSALVELINUSFONTINALISTHEIRHYBRIDOFFSPRINGSCIDATEYUANGUOSUPERVISWENGEZHAOPROFESSBINGQIANWANGPROFESSSPECIALITYZOOLOGYDATEOFDEFENCEMAY，2015DEGREECONFERRINGINSTITUTIONHARBINNMALUNIVERSITY

簡介：東北農(nóng)業(yè)大學碩士學位論文美洲擬鰈抗凍蛋白基因植物表達載體的構建姓名李樹峰申請學位級別碩士專業(yè)基礎獸醫(yī)學指導教師嚴云勤200251李樹峰ABSTRACT2002年5月CONSTRUCTIONOFPLANTEXPRESSIONVECTORHARBORINGPSEUDOPLEURONECTESAMERICANUSANTIFREEZEPROTEINGENEABSTRACTTHEANTIFREEZEPROTEINSHAVEBEENFOUNDINMANYORGANISMS，SUCHASFISHES，PLANTSANDINSECTSANDTHEANTIFREEZEPROTEINSINFISHESARESTUDIEDINDETAILTHEANTIFREEZEPROTEINSHAVEMANYAPPLICATIONSININCREASINGTHEQUANTITYANDQUALITYOFCROPIMPROVINGTHESEEDLINGLIVIABILITY，PROLONGINGTHEGROWTHSEASONOFCROPANDEXTENDINGTHEPLANTINGRANGE，ASWELLASDECREASINGTHESTOREDTEMPERATUREOFCROPTOPROLONGTHESTOREDTIMEMOREOVER，THEREAREMANYIMPORTANTAPPLICATIONSINMEDICINE，F(xiàn)OODSTUFFSCIENCEANDINDUSTRYINOUREXPERIMENT，ALOTOFREGULATORSEQUENCESINCLUDINGTHE35SPROMOTERWITHDOUBLEENHANCERELEMENTSANDTHETERMINATORNOSTHENSEQUENCEANDKOZAKSEQUENCEFORIMPROVINGTHEGENETICSTATEMENTINTHELEVELOFTRANSLATION，WEREAPPENDEDONTHETWOSIDESOFTHEARTIFICALLYPSEUDOPLEURONEETESAMERICANUSANTIFREEZEPROTEINGENEBYASERIESOFPROCESSANDTHEPRODUCTSWEREINKEDINTOPBI】21】TOGETTHEEXPRESSIONVECTORFORTHEANTIFREEZEPROTEINGENEFOLLOWEDBYIMPORTINGITINTOTHECALUUSOFMAIZEBYGENEGUNATLAST，WEDETECTEDTHEEXPRESSIONOFGUSGENELINKEDTOANTIFREEZEPROTEINGENETHERESULTINDICATESTHATGUSGENEISEXPRESSEDSTRONGLYANDINSTANTANEOUSIYINCALUUSOFMAIZEKEYWORDCARRIERDESIGNANTIFREEZEPROTEINREGULATORSEQUENCE

簡介：中文摘要黃腹?jié)撓壚O蜂OPIUSCARICIVORAEFISCHER是美洲斑潛蠅LIRIOMYZASATIVAEBLANDCHARD幼蟲．蛹期的一種重要內(nèi)寄生蜂。本文在實驗室條件下用菜豆PHASEOLUSVULGARISL．上的美洲斑潛蠅幼蟲作為寄主，對該蜂的生物學、生態(tài)學特性做了研究。研究結果如下1黃腹?jié)撓壚O蜂幼蟲為6齡，一齡幼蟲特化成具顎型，并相互攻擊，以后5齡幼蟲為顯頭型，蛹為離蛹，在寄主蛹內(nèi)結繭化蛹。并詳細記述了各蟲態(tài)的形態(tài)特征。2測定了17、21、25和33℃下卵、各齡幼蟲和蛹的發(fā)育速率，線性日度模型和TLOGISTIC模型均能很好擬合各蟲態(tài)的發(fā)育速率和溫度的關系。／在實驗溫度下，卵、幼蟲、L蛹和卵一蛹的發(fā)育歷期變化分別為2．54～7，69、0．5肚2．73、7．24～13，09和14．47～32．43天，發(fā)育起點溫度分別為11．48。C、7．74～12．80℃、1．60℃、8．03℃，有效積溫分別為41．32、10．27～17、89、208．33、303．03日度。蛹在低溫下就可以開始發(fā)育，由此可以推斷，在田間，該蜂可能以蛹越冬。在33。C69．8％的蛹休眠。整個幼期的存活率和溫度的關系符合雙曲線方程SO．009嚴O．410T．3．872震0．9583。卵的存活率在89．6％以上，幼蟲的存＼活率在81．3％以上，蛹的存活率變化幅度大，25。C時為95．5％．334C時只有8．7％。尹一F，F(xiàn)3成蟲主要在白天羽化，羽化高峰期在800～1000，且多數(shù)9L％當日交尾，羽化出的成蜂性比明顯偏雌性早61．511；在17“C一336C下，提供蜂蜜液時，雌蜂和雄峰的壽命隨著溫度的升高而縮短，雌蜂的壽命明顯長于雄峰的壽命，分別從17。C時8L天和47天降到33。C時的14天和13天；在25“C下，黃腹?jié)撓壚O蜂在羽化后12小時體內(nèi)已懷有卵，羽化后2～7日齡達到懷卵高峰期，在第三天達最大值，平均每雌懷卵數(shù)為24．1粒，之后，逐漸減少。雌蜂羽化后L2天即可產(chǎn)卵，即使不交尾，該蜂也有產(chǎn)卵能力。在21。C29。C溫度下，產(chǎn)卵高峰期在27日齡，平均產(chǎn)卵量隨溫度變化的方程為Y．O．057X22．728X一20．601R2O．934，而且成蜂取食也能致死寄主。另外，本論文對雌蜂的寄主搜索、產(chǎn)卵和寄主取食行為做了比較詳細的描述。Z一等凈生殖率奶在21℃達到最大114，7，隨溫度升高或降低，‰均減少；內(nèi)頻增長率‰和周限增長率九均隨著溫度的升高而上升，兩參數(shù)均在29“C達到最大值，隨后又逐漸下降。序5℃和29。C的RM值大約是17“C1拘1倍。種群趨勢指數(shù)和溫度呈拋物線關系，回歸方程為，一0．904，41．166T．374．320冠20．993．種群增長趨勢指數(shù)在25“C最大94．21，而33。CIJ種群趨勢指數(shù)0．78小于1，說明33“C時黃腹?jié)撓壚O蜂種群將減少。STUDIESONBIOLOGYANDECOLOGYOFOPIUSCARICIVORAEFISCHERHYMENOPTERABRACONIDAE，APARASITEOFLIRIOMYZASATIVAEBLANDCHARDDIPTERAAGROMYZIDAEABSTRACTOPIUSCARICIVORAEFISCHERISAMAJORLARVALPUPALENDOPARASITOIDOFLIRIOMYZASATIVAEBLANDCHARD．THEBIOLOGYANDECOLOGYOFTHISPARASITOIDWERESTUDIEDUNDERLABORATORYCONDITIONBYUSINGL．SATIVAEONTHEBEANPHASEOLUSVULGARISLASTHEHOST．THELALNAEOFO．CARICIVORAEUNDERWENTSIXINSTARS．THE1STINSTARLARVAEWEREMANDIBULATEWHILETHELARVAEOFOTHERFIVEINSTARSWEREHYMENOPTERIFORM．THELSTINSTARLARVAECOULDARACKEACHOTHER．THE6THINSTARLARVAESPUNACOCOONINTHEHOSTPUPAWHEREITPUPATED．THEMORPHOLOGICALCHARACTERSOFTHEPARASITOIDLARVAEOFEACHINSTARSWEREDESCRIBEDJNDETAIL．THEADULTPARASITOIDSEMERGEDBYDAYLIGHTWITHAPEAKAT8001000．ABOUT91％OFTHEADULTSMATEDONTHEEMERGENCEDAY．FEMALEBIASEDADULTSEXRATIOWASFOUNDINLABORATORYPOPULATIONSF旱61．5111．LONGEVITYOFBOTHFEMALEANDMALEADULTSDECREASEDWITHINCREASEOFTEMPERATURESFROM17℃TO33℃WHENHONEYBEESOLUTIONWASPROVIDED．MEANLONGEVITYOFFEMALESWASLONGER8LDAT17℃AND14DAT33℃THANTILATOFMALES47DAT17℃AND13DAT33。C．FEMALEADULTSSTARTEDOVIFLOSITIONON12DAFTEREMERGENCEWITHAPEAKON27DFROM21℃TO29℃．THERELATIONSHIPBETWEENAVERAGEFECUNDITYANDTEMPERATURECOULDBEFITTEDWITHTHEEQUATIONY；．0．057X。2．728X．20．601FR20．934．THEFEMALESWERE如LETOKILLHOSTSBYFEEDING．INADDITION．THEHOSTFEEDING，OVIPOSITIONANDHOSTSEARCHINGBEHAVIORSOFTHEPARASITOIDSWEREDESCRIBED，THEDEVELOPMENTALANDSURVIVALRATESOFTHEEGG．1ALVALANDPUPALSTAGESWEREMEASUREDAT5CONSTANTTEMPERATURESOF17，21，25。29AND33℃．THERESULTSSHOWEDTHATTHEIINEARDEGREEDAYANDTHELOGISTICMODELSCOULDDESCRIBEWELITHERELATIONSHIPBETWEENTHEDEVELOPMENTRATEOFEACHIMMATURESTAGEANDTHEMIDTEMPERATURES．THEDEVELOPMENTDURMIONSOFEGG，LARVA，PUPAANDEGGPUPAVARIEDFROM2．54TO7．69，0．50TO2．73，7．24TO13．09，14．47TO32．43DAYS，RESPECTIVELY．THEDEVELOPMENTTEMPERATURETHRESHOLDFORPUPAWAS1．60℃WITHEFFECTIVEACCUMULATEDTEMPERATURES208．33DAYDEGREE．SUGGESTING血ATTHEPARASITOIDCOULDOVERWINTERBYPUPAINTHEFIELD．NLEDEVELOPMENTTEMPERATURETHRESHOLDSFOREGG．1ARVAANDEGGPUPAWEREL1．48，7．74～12．80AND8．03℃WIMEFFECTIVEACCUMULATEDTEMPERATURES4I．32，10．27～17，89AND303．03DAYDEGREE．RESPECTIVELY．ABOUT69．8PERCENTAGEOFPUPAEUNDERWENTDORMANCYATORABOVE33℃．THERELATIONSHIPBETWEENSURVIVAIRATEANDTEMPERATURECOULDBEFITTEDWITHTHEEQUATIONS一0．009R0．410T3．872F尺O．9583．THESURVIVALRATESOFTHEEGGANDIARVAEWEREMORETHAN89．6％AND81_3％，RESPECTIVELY．PUPALSURVIVALRATEVARIEDGREATLYFROM8．7％AT33℃TOABOVE83．0％FROM17℃TO29℃．THENETREPRODUCTION‰PEAKEDAT21℃ANDDECLINEDBOTHABOVEANDBELOWTHISTEMPERATURE．HOWEVEGBOTHTHEINTRINSICRATEOFINCREASERMANDFINITERATEOFINCREASE¨INCREASEDFROM17。CTO29℃ANDDECREASEDFROM29℃TO33℃．THEINTRINSICRATEOFINCREASERMAT21℃OR25℃NEARLYDOUBLEDCOMPAREDWITHTHATAT174C．THERELATIONSHIPBETWEENTHE

簡介：湖南農(nóng)業(yè)大學碩士學位論文美洲商陸抗病毒蛋白基因在畢赤酵母中的表達姓名周倩申請學位級別碩士專業(yè)植物病理學指導教師高必達王錫鋒200361ABSTRACT1LANTVIRUSDISEASEISONEPRIMARYCROPDISEASEITDISTRIBUTESWILDLYANDDOESGREATHARMTOECONOMYOFAGRICULTUREYIELDPEOPLESEARCHAFTERWAYSTOPREVENTIONANDC11REVIRUSDISEASESFORLONGTIMEBUTNOTDEVELOPEFFECTIVEREAGENTYETMANYSTUDIESREVEALEDTHATPOKEWEEDANTIVIRALPROTEINPAPCANCONTROLPLANTVIRUSEFFECTIVELYBUTPAPINNATUREISLIMITEDWHICHCOULDNOTAPPLYCOSMICALLYUSINGBIOREACTERTOPRODUCEPAPISONEEFFICACIOUSWAYTOGETAMASSOFPAESOMEONETRIEDTHISWAYBUTTHEYIELDISLOWFRANCISEXPRESSEDTRUNCATEDMUTANTPOKEWEEDANTIVIRALPROTEINGENEINPICHIAPASTORISTHEYIELDREACHED1012MG／LDRCHENDINGHUINPLANTVIRUSGROUPOFINSTITUTEOFPLANTPROTECTIONOFTHECAASCHINESEACADEMYOFAGRICULTURESCIENCESLSELECTEDPAPMUTSRECOMBINANTSANDWANTEDTOPRODUCEPAPTHROUGHFERMENTATIONTOPREVENTIONANDCUREPLANTVIRUSDISEASEBUTASTOMUTSRECOMBINANTSTHEPERIODSOFFERMENTATIONISTOOLONGANDTHEEFFICIENCYOFUSINGMETHANOLISLOWSOUSINGMUT5RECOMBINANTSTOPRODUCEPAPINFACTORYISNOTFELICITOUSNESSLINEARIZEDTHEEXPRESSIONVECTORPPIC9KPINCLUDINGTHETRUNCATEDMUTANTPOKEWEEDANTIVIRALPROTEINPAPGENEBYRESTRICTIONENDONUCLEASESAL1ANDTRANSFORMEDITELECTRICALLYINTOPICHIAPASTORISGSLL5MUT十RECOMBINANTSWERESELECTEDBYPCRANDHIIGHYIELDMUTRECOMBINANTWASPICKEDOUTBYDOUBLEFILMIMMUNOBLOTTINGTOFINDOUTTHEDIFFERENCEBETWEENMUTANDMUTSRECOMBINANTSWECOMPAREDTHEIREXPRESSIONOFPOKEWEEDANTIVIRALPROTEININTHESAMECONDITIONSSDSPAGERESULTSSHOWEDTHATTHEREWASACLEARTARGETPROTEINBANDINMTITRECOMBINANTSUPERNATANTAFTER48HOURSOFCULTURING，WHILEAFAINTBANDONLYINMUTSRECOMBINANTAFTER72HOURSWESTERNBLOTTINGRESULTSHOWEDTHATTHEREWASNEMARKABLEDIFFERENCEOFYIELDBETWEENMUTANDMUTSRECOMBINANTSAFTER6DAYSINDUCEDANTIVIRUSACTIVITYTESTSREVEALEDTHATCULTURESUPEMATANTSOFMUTANDMUT8RECOMBINANTSCOULDINHIBITTMVINFECTIONWITHHIGHEFFICIENCYINTHESAMECONCENTRATIONANDTHEREWASNOSIGNIFICANTDIFFERENCEBETWEENTHEMDIFFERENTCULTIVATIONCONDITIONSUCHASINDUCEDTIME，METHANOLCONCENTRATIONANDPHOFCULTUREMEDIUMWASSTUDIEDATSHAKEFLASKCULTIVATIONSDSPAGERESULTSSHOWEDTHATASTOMUTRECOMBINANTHIGHESTYIELDWASOBTAINEDAFTER4DAYSINDUCINGANDWITHTHECULTURE

簡介：福建農(nóng)林大學碩士學位論文美洲大蠊誘捕器及誘餌的研究姓名周玉峰申請學位級別碩士專業(yè)農(nóng)業(yè)昆蟲與害蟲防治指導教師吳珍泉20040401THERESEARCHOFTRAPANDHAITTOPERIPLANETAAMERICANAABSTRACTINTHISARTICLE，THEBEHAVIORRESPONSEOFPERIPLANETAAME，LCANATOCOLOR、ROUGHNESSANDBARRIERWASSTUDIEDITWASFOUNDTHAT只AMERICANATENDSTOSETTLEROUGHERSURFACE，BEBLUNTTOTHECHANGEOFCOLOR，F(xiàn)AVORMOVINGABOVEIFTHEANGLEBETWEENTHEBARRIERANDTHEMOVINGDIRECTIONOFROACHESEQUALSTO115。ORLARGER，95％OFTHEROACHESWILLCONTINUETOMOVEFORWARDTOTHESIDEOFTHEOBTUSEANGLETHEANGLEBETWEENBAFFLESANDTHEBOTTOMBRIMOFTHETRAPWASDESIGNED25。THEOBTUSEANGLEIS115。THATLEADSMOSTCOCKROACHESMARCHINGINTOTHETRAPTHELENGTH，WIDTHANDWEIGHTOFPAMERICANAIMAGOWEREMEASUREDTHELENGTHBETWEENTWOPOSTPEDESIS213～264CMWITHTHEAVERAGEOF241CMITSBODYLENGTHIS348～448CMWITHTHEAVERAGEOF385CM，ANDITSWIDTHISL_15～163CMWITHTHEAVERAGEOF148CMTHECOCKROACHTRAPHASFOURSMALLDOORSTHATROACHESCANGETIOBUTNOTOUTONTHEBASISOFCOCKROACHES’BODYLENGTHANDWIDTH，THESEDOORSARE28CMINLENGTHAND2OCMINWIDTHTHECOCKROACHTRAPWASDESIGHEDTHREESHELVESTHATCAMBETAKEAPART，THECENTRALOFBOTTOMLAYERWASUSEDTOPLACEBAITTHATATTRACTSCOCKROACHESCRAWLINGTHROUGHSLOPEOFBOTTOMTOTHEMIDSTLAYERTHEMIDSTLAYERHASSMALLDOORSANDGROOVEWHICHFORMSLOPETHETOPLAYERWASALUCIDITYCOVERITWASFOUNDTHATTHEAPPETITEOFPAMERFCANAIMAGOAEHIEVESTHEMAXIMUMWHENITSDENSITYIS5774PERSTERETHECOCKROACHTRAPLURESROACHESWITHNATURALATTRACTANTSWHICHARECORN，LEGUME，VEGETABLE，ANDOTHERORDINARYFOODINDIANMEAL，POWDERYYEAST，HONEYANDBUTTERWERESELECTEDTOMAKEBAITAORTHOGONALDESIGNWASMADETOABTAINTHEBESTPROPORTIONOFTHEINGREDIENTSOFTHEBAIT，THEOPTIMUMPROPORTIONOFINDIANMEAL，POWDERYYEAST，HONEYANDBUTTERWAS6323THEOPTIMUMMOISTUREIS5325％KEYWORDSPERIPANETAAMERICANATRAPBAIT

簡介：東北農(nóng)業(yè)大學博士學位論文國內(nèi)外PRRSV美洲型毒株結構基因序列分析及感染豬病理和細胞免疫變化的研究姓名侯振中申請學位級別博士專業(yè)獸醫(yī)外科學指導教師王林安200361了國內(nèi)流行的SD毒株和D。毒株毒株均屬于美洲型，與美洲型毒株具有高度的同源性。通過確定病毒侵害細胞和組織的種類和部位，為有針對性診斷指明采樣方向。并通過監(jiān)測對PRRSV感染豬細胞免疫和體液免疫，了解了PRRSV感染時豬體免疫功能的變化規(guī)律。這不僅為疫苗研制提供分子生物學基礎，而且對建立快速的臨床病理學診斷和合理的免疫方案以及高效靈敏的診斷方法創(chuàng)造良好條件。關鍵詞PRRSV；序列分析；FIA；病理組織學；組織分布CD4；CD8L／。1|T／T。。，IL

簡介：南京林業(yè)大學碩士學位論文美洲黑楊不同無性系對分月扇舟蛾的抗性及其機理姓名方杰申請學位級別碩士專業(yè)森林保護學指導教師趙博光20040601RESIS協(xié)NCEOFCERBINCIONESOFPOPU，USD臺，幻IDBSTOIAR、舊OFC，OS括餾A仃ASFOMOS，SAND№MECHANISMABSTRACTTKP即ERFIRSTSTUDIEDTLLERESISTAⅡCEOF18CLONESOF凡P“Z淞出幻洫占T0LARVAOFC協(xié)把RⅡ繃口占幼N伽虹NO1TONO18R印∞SEI刪9544、9610、10214、1042、8614、892、893、897、9430、8116、9412、401、451、8122、744、8698、868、669DONES礎巾ECTIVELY’I72WASCHOSEN雛CONTR01NEIRIRMECHANISMSWEREALSOSTUDIEDITFOUNDEDSCIENT詬CBASISFORSELECTM窟NEWPOPLARS廿AILLSTHEMAINRESULTSARC髂FOUAWS1SI姐IFICAILTDIFFERENCEOF∞SISTANCEINCENAINCLONESOFP印“M5如蛔赴婦TOAO贍R口礬甜幻辨D幽EXISTEDHIBOMCHOICE齟DNONCHOICETESTS。LEAFARCASFEDBYTLLELAN，艟OF脅鯽位翻D礎脯D占括WEREMORENL姐THATOFCON缸DLINNO9、11、15CLONESS觚STICALLY，BUTLESSTHAILTHATOFCON仰LIN№1、2、3、4、6、7、8、12、13、16、17CLONESS乜TISTICALLYIHEREWEREN0SIGRIIFICAMDIFF打鋤CEIN1EAFAREAFEEDINGBE腳EENNO10、14、18CLONESANDNLATOFCONⅡD1THERESULTS址燈幢INDIC曲ED血ATNO1、2、3、4、6、7、8、12、13、16、17C10NESARERESIS啪TCLONES鋤DNO9、10、11、14、15、18CLONESA化姐S∞叫VECLONES2DI觸犯NTIALEFF酏TSOFCERTAINCLONESOFPDP“M5如FFD血據(jù)S衄THCGROWMANDDEVELODMCNTCXISTEDMLARV躚OFCLDS陽R口印娜FDMD工曲SHOWEDINMEVARIESOFTHEMAINIRLDIC種ORSOFGROW出AILDDEVEL叩INCNT，SUCHASLA“∞WEIGHT，WEIGHTGROWMPERDAY趾DRGRRELATIVE掣DWMRATENO。7DONE，WTIICHHADMGH豇RESISTANCEEXPRESSEDAS舡NOUN￡OFLCAFF毫EDIN窖，SHOWED蜘啦BI鉚EFF如TSOFGROWTLLANDDEVELOPMENTONLARVAEOFAD占細Ⅵ伽肋MDJ婦SIGNIFICANTLYHOWEVERNO1CLO艙，WHICHHADLLIGHERSUSCEPTIVITYHADPOSITIVEEFFBCTT0TIIOSESI冉ⅢICALLTLY3DI№DALE壤鷺TSOFCERCAINCLONESOF尸臼口““S如蛔娩SONF00DINTAKESANDF酏ESEXCRETIONINLARVACOF伽據(jù)，口口拙咖MDS婦EXISTEDNO7DONESHOWEDIMLIBITORYE丘色CTSONFOODINT址∞SAILDFBCESEXC刪ONOⅡ1ARVAEOFAD占據(jù)R口口N珊FDMDS括SIG湎C枷YHOWEVER’NO1CLONE，WHICHHADHI曲鐘SUSCE兩V姆HADPOSILIVE胡KTT0也OSESIGLLI6CANNY4ECIEFFICIENCYOFIR增RESTED0FFBODAILDECDE矗ICIENCYOFCONVERSIOⅡOFDIGESTCDFOODOFLAN，AEOFC如工把R扭口，如占細MDS括AFT盯FH舶GWI血NO15CLONEINCREASEDSⅢSTICALLYITINDICATEDMATTILE∞KINDOFCLONESCOULDPROMOTETHEGROW山躕DDEVELOPNLENTOFLA“AOFA珊捃陽DM鼬，M∞括SIGLLIFIC姐DYHOWCV％METWOINDICATOI。SOF1ARV∞OFCF∞膪M礬D占幻M甜婦柵F曲MNGWIM№7CLONEDECRCA∞D(zhuǎn)螄MSTICALLYINTTLESCCONDDAY’BUTNOSIGILIFICAILTDECRE鼬EWEREOBSERVEDINTHEFOUOWIⅡGDAYS，TLLECAUSESSHDULDBESMDIED脅HER5111MOSTCASESINMESTUDV’RESIST趵CEOF吐LECLONES鈀STEDIIL尸9礎F船婦FFDID昭TO1ARVAOF

簡介：中國農(nóng)業(yè)大學博士學位論文美洲商陸抗病毒蛋白Ⅰ和Ⅱ基因功能的研究及葉片中抗病毒蛋白的分離姓名趙玉申請學位級別博士專業(yè)植物病理學指導教師李懷方王錫鋒20040501ABSTRACTPOKEWEEDANTIVIRALPROTEINBELONGSTIBOSOMEINACTIVEPROTEINS限IPS隊PHASPOTENTANTIVIRALACTIVITYAGAINSTMANYPLANTVIRUSPTLWASUSED∞THETEMPLATETOAMPLIFYTHEDELETEDMUTANTPAPGENENDELETEDANDN一，CDELETEDBYPERANDTHENTHETWOGENESWERECLONEDINTOEXPRESSIONVECTORDIFFERENTIPTGINDUCIBLEEXPRESSIONVECTORCONTAININGDIFFERENTDELETEDMUTANTPAPGENEWASCONSTRUCTEDANDTRANSFERREDINTOECOLISTRAINBL21DE3一PLYSSTHETWOSPECIFICPROTEINSWEREPRODUCEDAFTERINDUCTIONANDTHEIRMOLECULARWEIGHTWERE32KDAND29KDACTIVITYDETECTIONTESTSREVEALEDTHATTHETWOPROTEINSCOULDINHIBITTMVINVASIONWITHHIGHEFFICIENCYTHEDELETEDMUTANTPAPGENEWASALSOCLONEDINTOPLANTEXPRESSIONVECTORPBLL21ANDTRANSFORMEDINTOTOBACCOUSINGLEAFDISCMETHODMEDIATEDBYTHEAGROBACTERIUMTUMEFACIENSTRANSGENICTOBACCOPLANTSWEREOBTAINEDTHROUGHSEREANINGWITHKANAMYCINTHETRANSGENICTOBACCOPLANTSCOULDDELAYTMVINFECTIONFORABOUT25DAYSCOMPAREDWITHNONTRUNSGENICTOBACCOPLANTSPOKEWEEDANTIVIRALPROTEINIIPAPIIISEXPRESSEDWITHHIGHLEVELINSUMMERLEAVESTHEEXPRESSIONOFPAPIIISREGULATEDBYSEASONTHETOTALRNAWASEXTRACTEDFROMPOKEWEEDPHYTOLACCAAMERICANALLEAVESINSUMMERUSINGTHEMETHODOFTRLZOLANDUSEDASTEMPLATETOAMPLIFYTHEPAPIIGENEBYRTPCRANDTHENTHEGENEWASCLONEDINTOECOLIEXPRESSIONVECTORANDSECRETEDEXPRESSIONPPIC9KVECTORTHETWOVECTORSWITHPAPLLGENEWERETHENTRANSFERREDINTOECOLISTRAINBL21DE3PLYSSANDPACHIAPASTORISGSLL5STRAINRESPECTIVELYTHESPECIFICPROTEINWASPRODUCEDINDUCEDBYIPTGANDMETHAN01SDSPAGERESULTSSHOWEDTHATONLYONEPROTEINBANDEXISTEDBYINDUCTION，ACTIVITYDETECTIONTESTSREVEALEDTHATTHETWOPROTEINSCOULDINHIBITTMVINVASIONWITHHIGHEFFICIENCYTHEREARESOMEISOFORMSOFPAPSSHOWINGINHIBITORYABILITYTOVIRUSINFECTIONINVARIOUSORGANSANDATDIFFERENTSTAGESOFDEVELOPMENTTHREEDIFFERENTSPECIESOFTHEPROTEINⅢBEISOLATEDFROMLEAVESOFPOKEWEEDPAPPAPIIANDPAPIIIPROTEINSWEREPRECIPITATEDWITH5080％AMMONIUMSULFATEFROMLEAVESINSPRING，SUMMERANDAUTUMNRESPECTIVELYPROTEINSWEREPURIFIEDONSPCATIONEXCHANGETHENONMQANIONEXCHANGEANDFINALLYONMSCATIONEXCHANGEORONMQANIONEXCHANGEWEFOUNDTHATSOMENEWPOKEWEEDANTIVIRALPROTEINSSEPARATEDFROMTHESELEAVESWEREDIFFERENTFROMPAPPAPIIANDPAPIIIBUTTHESENEWPROTEINSNEEDTOBESEPARATED，PURIFIEDANDSTUDIEDFURTHERFIVEDIFIERENTSPECIESOFTHEPROTEINCALLBEISOLATEDFROMLEAVES，SEEDSANDROOTCULTURESINTHEPRESENTSTUDYNONEWASISOLATEDFROMROOTTHENUCLCOTIDESEQUENCESOFTHEPAPGENEANDPAP【IGENEINTHEROOTOFPHYTOLACCAAMERICANAWEREDETERMINEDBYREVERSETRANSCRIPTIONPOLYMERASECHAINREACTIONRTPCRANDPCRTHEGENECOMPRISES942BPAND933BPRESPECTIVELYENCODING313AMINOACIDSAND310AMINOACIDSWHENPAPGENEANDPAPLLGENESEQUENCESINTHEROOTWERECOMPAREDWITHTHOSEINTHESPRINGLEAVESANDSUMMERONESTHEHIGHESTSIMILARITY100％AND998％WEREOBSERVEDRESPECTIVELYTHEYBOTHHADONEOPENREADINGFLAMEORFANALYSISOFTHETWOSEQUENCESSHOWED，INFACT，THEYBELONGEDTOONESPECIESKEYWORDSPHYTOLACCAAMERICANALPOKEWEEDANTIVIRALPROTEIN，RIBOSOMEINACTIVITINGPROTEINS，EXPRESSION，PURIFICATIONOFPROTEIN

簡介：浙江大學碩士學位論文黃腹?jié)撓壚O蜂對美洲斑潛蠅的生理調(diào)控機制研究姓名萬志偉申請學位級別碩士專業(yè)農(nóng)業(yè)昆蟲與害蟲防治指導教師余虹陳學新20050501致謝本論文的順利完成全得益于各位老師、同學及朋友們的指導和幫助。首先，我要向兩位尊敬的導師一余虹副教授和陳學新教授表示衷心的感謝與崇高的敬意三年來，兩位導師在學習、科研和生活各方面都給予了極大的關心和幫助，從選題、設計到論文的寫作無不凝聚著導師大量的心血和汗水。兩位導師學識淵博，治學嚴謹，待人誠懇，為人正直，更以敏銳的學術思想深深影響著我，激勵我不斷進取。在此要感謝何俊華教授、施祖華教授、徐志宏教授、馬云高級實驗師存論文研究過程中給予的多方面的幫助和指導。感謝本所劉樹生教授、葉恭銀教授、張傳溪教授、莫建初教授、婁永根教授、祝增榮研究員、蔣明星教授、姚洪渭副教授等老師在論文的開題報告、讀書報告、預答辯以及學習和實驗中給予的關心和幫助。本校電鏡室的洪健、徐穎、黎軍英等老師在樣品制備、電鏡觀察以及照片沖洗方面給予了精心的指導和極大的幫助，核農(nóng)所的孫志明等老師熱情幫助輻射處理寄生蜂。三年的求學時光，本實驗室的自素芬、潘健、尹承山、余曉霞、酈衛(wèi)弟、時敏、吳瓊、杜彩裕、王義平、張紅英、汪海燕、李芳芳、榱鵬、俞瑞鮮、黃芳、魏書軍、陳亞鋒、狄蕊、孟祥鋒、徐遙、朱金文和實習生姚剛，應用昆蟲研究所的林祥海、張建國、蔡霞、臧連生、吳志毅、李俊敏、李增梅、王慧、張忠、陳亮、滕立、張小斌、李江紅等同學對我的幫助表示感謝。衷心感謝室友李紅亮、葉昱程和施衛(wèi)兵同學臼常的關心和幫助。我還要感謝我的父母、姐姐和親友對我的理解、支持和鼓勵，是他們始終如一的關心和愛護使我能安心學習。不斷進取。在這里也一并向前面未提及的多年來一直關心和幫助我的許多老師、同學和朋友們表示感謝。最后感謝論文評閱人和答辯委員會的專家在百忙之中給予我的寶貴的建議和指導。謹以此文獻給所有關心、幫助和愛護我的老師、親友和同學們。萬志偉2005年5月于華家池校區(qū)

簡介：東北林業(yè)大學博士學位論文中國山楊與美洲山楊雜交種新品系選育研究姓名邢亞娟申請學位級別博士專業(yè)森林培育林木遺傳育種指導教師楊傳平張含國20050501童塑堡壘呈墼絲耋竺篁圣ABSTRACTTHISRESEARCHPROJECTISTHEFIRSTLARGESCALEANDSYSTEMATICTOCARRYTHROU曲BREEDINGABOUTPDAVIDI冊口PTREMULOIDESTHEADVANCEDINTERNATIONALBREEDINGPROCEDURE“BSCS”WASADOPTEDTOCARRYOUTGEOGRAPHICDISTANTCROSSING，ANDTHENEWGOODCLONESWERESELECTEDIMPROVINGTHEGENETICSCHARACTEROFPDAVIDIANAHASMOREIMPORTANTVALUEBYTHERESEARCHONMICROBODYPROPAGATIVETECHNIQUES，THEOPTIMIZEDSYSTEMTHATWASSUITABLETOCLONEBREEDINGOFPDAVIDIANAPTREMULOIDESWASBUIIT，WHICHCOULDRESOLVETHEDIFFICULTIESOFCLONEBREEDINGANDLOWPROPAGATIVERATEOFPOPULUSDAVIDIANATHERESULTOFTHETHESISISASFOLLOWSTHEFIRSTLYBYMEANOFCROSSINGBREEDINGANDANOTHERREAIITIVETECHNIQUES，THEEIGHTGOODFAMILIESTHETENGOODSINGLETREESANDTHEFOURCHOICENESSHYBRIDCLONEWERESELECTED，WHICHWEREID421，GD421，CA421，GH322THEHYBRIDID42一L，GD42一1WERETHETRIPLOIDTHOSEFOURWEREGROWINGFAST，ANDTHETREEHEIGHTOF7YEARSOLDOVER95MTO1O5M，DBH87CMTOL09CM，VOLUMEO0255M5TOO0448M5；ANDTHENEWCLONESWITHHIGHRESISTANCEANDNOFREEZEINJURYHAVEVERYGOODPROPAGATIVEPOTENTIALTHESECONDLY，THECULTUREMEDIUMWMWASFEASIBLEBEGINNINGCULTUREMEDIUM；THEIMPROVEDCULTUREMEDIUMWMWASFITFORCONTINUOUSPASSAGEMULTIPLICATIONTHEMUL卻LICAFIONCOEFFICIENTWAS11～15；DURINGTHETISSUECULTUREOFPDAVIDIANAX只TREMULOIDES，BOTHCARTESUGARANDAGARHADINFLUENCESONVITRIFICATIONTHEBESTCANESUGARANDAGARCOMBINATIONWASCANESUGAR79／L，AGAR30蟣HIGHTEMPERATURE，PLASTICFILMOFBADVENTILATENATUREANDINSUFFICIENTLIGHTINTENSITYACCELERATEDVITRIFICATIONSEEDLINGSPRODUCINGREMARKABLY；THEIMPROVEDCULTUREMEDIULNW／VIAND1／2MSWEREFITFORROOTINGCULTUREOFHYBRIDCLONETHEROOTINGRATEWASUPTO928％～93F3％BYMEANSOFIMPROVINGTHETISSUECULTUREPROGRAMME，THEWANDCUTTAGEWASUSEDINTOTISSUECULTURETHESETECHNIQUESOVERCAMETHEDIFFICULTPROBLEMSOFASEXUALPROPAGATIONOFZDAVIDIANAXPTREMULOIDES，CURTAILINGTHEBREEDINGPERIOD，OPTIMIZINGTHEPROPAGATIONPROGRAMMETHETHIRDLY，THESSRMOLECULARMARKERWASUSEDTOANALYSISGENETICSBIODIVERSITYOF3TYPEWITHPOPULUSDAVIDIANAWITH5SPECIALPRIMERS，THROUGHSHANNONINDEXANDNEI’SINDEXGUESSGENETICSDIVERSITYWECANMAKESURETHATTHEPOPULUSDAVIDIANAHAD廿LEHIGHESTGENETICSDIVERSITYANDTHESECONDWASPDAVIDIANAPTREMULOIDESXIAOXINGANLING，ANDTHELEASTWASPDAVIDIANAXPTREMULOIDESZHANGGUANGCAILINGUSINGTHEGENETICSDISTANCEMATRIXANALYSIS，ANDTHERESULTWASTHATONTHEBASISOFGENETICSDISTANCETHEEACHHYBRIDOFPDAVIDIANAXPTREMULOIDESWASCONGREGATEDASONESORTATFIRSTTHENWITHTHEPDAVIDIANAASONESORTKEVWORDSPOPULUSDCMIDIANA；POPULUSTREMULOIDES；CROSSINGBREED；ASEXUALPROPAGATION；MOLECULARMARKERII

簡介：獨創(chuàng)性聲明本人聲明所呈交的學位論文是本人在導師指導下進行的研究工作和取得的研究成果，除了文中特別加以標注和致謝之處外，論文中不包含其他人已經(jīng)發(fā)表或撰寫過的研究成果，也不包含獲得西南政法大學西南政法大學或其他教育機構的學位或證書而使用過的材料。與我一同工作的同志對本研究所做的任何貢獻均已在論文中作了明確的說明并表示了謝意。學位論文作者簽名簽字日期年月日學位論文版權使用授權書本學位論文作者完全了解西南政法大學西南政法大學有關保留、使用學位論文的規(guī)定。特授權西南政法大學西南政法大學可以將學位論文的全部或部分內(nèi)容編入有關數(shù)據(jù)庫進行檢索，并采用影印、縮印或掃描等復制手段保存、匯編以供查閱和借閱。同意學校向國家有關部門或機構送交論文的復印件和電子文檔。（保密的學位論文在解密后適用本授權說明）學位論文作者簽名導師簽名簽字日期年月日簽字日期年月日碩士學位論文以異化勞動為核心的法以異化勞動為核心的法哲學批判哲學批判基于社會公平正義角度解讀基于社會公平正義角度解讀18441844年經(jīng)濟學哲學手稿年經(jīng)濟學哲學手稿RESEARCHONMARX’SLEGALPHILOSOPHYCRITICISMOFWHICHESTRANGEDLABISTHECEANINTERPRETIONOF“ECONOMICPHILOSOPHICALMANUSOF1844”FROMTHEANGLEOFSOCIALFAIRNESSJUSTICE作者姓名江優(yōu)優(yōu)指導教師靳松副教授西南政法大學SOUTHWESTUNIVERSITYOFPOLITICALSCIENCELAW