煙堿降解菌的分離鑒定、降解特性及其降解途徑的初步研究.pdf

1、中南民族大學(xué)碩士學(xué)位論文煙堿降解菌的分離鑒定、降解特性及其降解途徑的初步研究姓名：孔雯申請學(xué)位級別：碩士專業(yè)：@指導(dǎo)教師：李曉華2011煙堿降解菌的分離鑒定、降解特性及其降解途徑的初步研究IIABSTRACTNicotineisamajalkaloidintobaccoPlants.Excessivelytakingintonicotinewillrestraincentralnervoussystemparalysethehearte

2、vencausedeathInmanuafeutringtobaccoproductswasteswithhighcontentofnicotineasthemaintoxiccompoundareproducedwhichserioulycontaminatetheenvironmentthenicotinecontentintheuppertobaccoleaffromourcountryaregenerallyhighsothat

3、unabletobeusedastobaccoblendwhichcausedwasteofresourceeconomiclosses.Thusexplinganeffectivewaytoreducethenicotinecontentincigaretteenvironmentisofgreatsignificancetohumanhealth.Interestinglymicrobeitsmetabolizedenzymecan

4、degradenicotinewhichisnotonlyhelpfulfhumanhealthbutalsoimprovethequalityofsmokeutilityoftobaccoleavesbringalotofeconomicbenefitsThisstudyutilizednicotineasthesolesourceofcarbon29bacterialstrainswasisolatedfromsoiltobacco

5、waste.Thedegradationefficiencytestshowedthatallofthestains’degradationefficiencyweremethan70%19strainsamongtheisolateswhosedegradationefficiencyweremethan90%.Furtherstudywasbasingon12strainswhichhavebeenisolatedbytheexpe

6、rimentalresultsofthemphological，physiologicalbiochemicalanalysisthecomparisonoftheir16SrDNAanalysisthese12strainswereidentifiedasArthrobactersp.Pseudomonassp.OchrobactrumintermediumAgrobacteriumtumefaciens.StudiesontheAg

7、robacteriumtumefaciensBKCER11strain’sdegradationcapabilityoptimizationoffermentationconditionswerecarriedout.FinalytheresultsshowedthattheBKCER11straincouldtoleratehighconcentrationofnicotineupto5gLwhentheconcentrationof

8、nicotineintheculturemediumwasgreaterthan5gLtheBKCER11strain’sgrowthwashighlyinhibitedthenicotineinthemediumcouldnotbedegraded.TheBKCER11strainnotonlyhasawidetolerancetopHbutalsopossessestheabilitytotoleratetemperature.Lo