香樟優(yōu)樹的分子標記和遺傳分析.pdf

1、福建農(nóng)林大學碩士學位論文香樟優(yōu)樹的分子標記和遺傳分析姓名：宋愛云申請學位級別：碩士專業(yè)：森林培育指導教師：陳輝2002.5.1香樟優(yōu)樹的分子標記和遺傳分析GeneticAnalysisandIdentificationofEminentTreesofCinnamomumCaphora億)PrestAbstractCinnamomumCaphora億JPrestisofhigheconomicvalueandasortofresource

2、ofspiceryItisalsousefulformedicaluse，landscapearchitectureandhighqualitytimberproductionbecauseithasthebeautifulGrowshapeandstrongresistancetoairpollutionEssentialoilcontainsLinaloolmainlyLinal001canconfectessenceNowCinn

3、amomzonCaphora陀)PreststillgrowwildlyInordertobuildupaexcellentclonalbaseofhighcontentLinalool，first，wemustselectandcategorizefinetreesThetraditionaltaxonomyofCinnamomumCaphora化)PrestisbasedonthecontentofLinal001butthecon

4、tentofLinaloolisaffectedbyenvironmentaJfactorsandthedevelopmentstageofplant，soitmakesitdifficulttodetectcorrectlyThegeneticdiversityofCinnamomumCaphoraf￡P(guān)re礬anddifferenceofeminenttreeswerestudiedbyRAPDMolecularmarkersThe

5、RAPDstandardizedprocedureofCinnamomumCaphora(L)PrestwasestablishedwhichsupplythepreciseandreliablemethodidentifiedtheCinnamomumCaphora犯JPresttrees。Thisresearchalsomakethecomparisonbetweenthetraditiona】taxonomicmethodandm

6、o／ecrlarmakersThemainresultsandconclusionareasfollows：1TheDNAextractionandpurificationofCinnamomwnCaphora伍JPrestisverydi搦cultybecauseofthehi曲levelofphenolsandtanninsubstancesTheimprovedSDSmethodwasputforwardinthisstudy2T

7、hereliableRAPDanalysissystemwasestablishedthereactionvolumeis20u1Reactionmixtureconsistwith50—80rigDNA，】50umoYLofeachdNT只2Ommol／LMgCl2，02umol／Lrandomprimerand15uTagpolymeraseRAPDprogramis：5minat94。Cforpredenaturation：the

8、n38cyclesof305at94℃fordenaturation，30Sat38“Cfuranneeding90sat72℃forextension；finallyextensionat72。Cfor7rain。3Nineteenprimersselectedfrom100primerstestedonthebaseofthenumberandfrequencyofpolymorphismswiththe19primers，145b

9、andswerepolymorphic(59％)4UPGMAclusteranalysiswascarriedoutusingsoftwareBiolDBendrogramwasconstructedbasedontheRAPDdataAccordingtothedendrogramtheaccessionscouldbeclusteredintofivegroupsatthe1evelofD=073Soitwasnotdifncult

10、toseethatthereweredistinctdifferenceandintemalrelationamongeminenttrees5AccordingtothecontentofextractedLinal001finetreesofCinnamomumCaphora億JPrestwereclassifiedbythemethodofthe1eastdistancenleresultcomparedwiththetaxono