擬南芥組成型抗病突變體對高溫脅迫應(yīng)答的研究.pdf

1、沈陽師范大學(xué)碩士學(xué)位論文擬南芥組成型抗病突變體對高溫脅迫應(yīng)答的研究姓名：姜延高申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：@20100101II Arabidopsis Mutants Characterized with Consti- tutive Resistance to Pathogens Responses to he- at stress Exposure Abstract Frequent human acti

2、vities emit large amounts of greenhouse gases to the air,and the levels increased gradually., leading to global climate warming, and the occurrence of extreme weather are becoming more frequently , such as the abnormal h

3、igh temperature happened in some areas which plays an important role in reducing the crop yields, it has already become a key environmental factor in the distribution, growth and productivity of the plants. In recent yea

4、rs, SA and its salts are considered to be a new type of plant hormones , as a signal molecule in the response to the stress, Currently, there were many reports about the SA in plant resistance. In this experiment, Arabi

5、dopsis wild type (Columbia) and its salicylic acid (SA)-altering mutants snc1 (suppressor of npr1-1, constitutive) with high SA level, transgenic line nahG with low SA level and npr1-1 (nonexpressor of PR gene) with SA s

6、ignaling blockage were used to reveal the mechanism by which the constitutive resistant mutants responses to heat stress in controlled conditions. All the tested plants were randomly divided into two groups, referred as

7、control group and heat stress group.They were trained within the incubators, the heat stress group, constant temperature 38 ℃, control group, 22 ℃ (light,) / 18 ℃ (dark), after 24h and 48h respectively ,collecting the da

8、ta of the content of chlorophyll and MDA, enzyme activities of SOD, POD and CAT in leaves. The results showed that at room temperature, snc1 morphologically smaller than the other Genotypes, indicating that the accumulat

9、ion of salicylic acid inhibit plant growth. 24 h after the heat treatment, the chlorophyll content of snc1 decreased, the other three increased, 48 h later, chlorophyll concentrations are all decreased, but the chlorophy

10、ll content of snc1 dropped most obviously. After 24 h heat stress, the-SOD activity of wt and npr1 increased, while the snc1 and nahG decreased, heat stress 48 h later, SOD activity has fallen for all lines, but the muta

11、nt snc1, nahG, and npr1 decline far more than the wild type, which is consistent with the shape phenotype these plants respond to heat. The snc1 had the highest POD activity relative to other lines in both unstressed and