連云港近海岸海洋沉積物中微生物群落結(jié)構(gòu)與功能的初步研究.pdf

1、江南大學(xué)碩士學(xué)位論文連云港近海岸海洋沉積物中微生物群落結(jié)構(gòu)與功能的初步研究姓名：朱廣超申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：李華鐘王淑軍20090801AbstraetAbstractLianyungangisallimportantportcityofChinaEnvironmentpollutionbecamemoreandmoreserious、析n1theeconomicdevelopmentinrecentyearsInt

2、hisstudythetypeofpollutantandthedegreeofpollutionin80samplesfromthreepillars(Z01，Z02，Z03)sampledfromdifferentinshoreareaswereanalyzed，theStnlCtureandthefunctionofthemicrobialcommunitywerestudied，thebacterialstrainswereis

3、olatedTheresultofmetalelementsanalysisshowedthatallthesamplesfromthreepillarswerepullutedwitharsenicandstannummoderatelyenrichedSimilarresultwerealsofoundwithbariumandzirconiuminZ01copperinZ02andbariuminZ03Themetalelemen

4、tsinthesedimentmainlyexistedintheformofresidue，followedbythestateofFeMnoxideandcarbonatecompound，thelessexistenceiStheexchangeablestateThemicrobialcommunitys缸1lctl鵬andthefunctiongenesofmalinesedimentsof22samplesfromthree

5、pillarswerestudiedbythemicroarrayTheresultshowedthatmicrobialcommunity咖ctIlreandthefunctiongenesweresignificantlydifferentamongthreepillarsZ01hasgreatestdiversityhavingmicrobialsfrom10differentgenerasuchasActinobacter，Ps

6、eudomonadalesandSOonForZ02andZ03，eachhasmicrobialsbelongingto6differentgenerasuchasBdellovibrionales，DesulfovibrionalesandSOonTheclusteringanalysisindicateddifferentsamplesofthesamepillarhadthesimilarfunction111ecanonica

7、lcorrespondenceanalysisonrelationshipbetweenthecompositionandfunctionofmicrobialcommunityandpollutionsbyanalyzingthecompositionanddynamicsofmicrobialcommunityandtheparametersofmainpollutionsfrommarinesedimentswereperform

8、edandtheresultsshowedthatthemicrobialcommunitystructureandfunctionweresignificantlyinfluencedbyzirconium，petroleum，conductivityandarsenic111eresultmaybenefittobioremediationofmarinepollutionTotalof51strainswereobtained，r

9、esultofphysiologicalandbiochemicalcharacteristicdeterminingand16SrDNAgenesiringappraisalindicatedtheybelongto17generasuchasBacill淞BrevundimonasandSOonBacilluscoveredthemajorityofisolatesAstrainresistanttocopperlcadandchr

10、omiumwasisolatedfrom51strainsseparatedfrommarinesedimentsThestrainwasidentifiedasArthrobacterIt’SbiologicalcharacteristicswerestudiedDifferentconcentrationsofheavymetaliOIlSextendedthelagphagetovariousdegreesThewaterishs

11、trainsCanadsorbcopper(II)andlead(II)by7722％，9543％，respectivelybutCannotadsorbchromium(VI)，andthischaracteristicswerestableafter10generationsKeywords：Marinesediment，Microbialcommunitystruture，functionalgenes，MicroarrayHea