藥用植物內(nèi)生芽孢桿菌的多樣性和系統(tǒng)發(fā)育研究.pdf

1、四川農(nóng)業(yè)大學(xué)碩士學(xué)位論文藥用植物內(nèi)生芽孢桿菌的多樣性和系統(tǒng)發(fā)育研究姓名：陳蘭申請學(xué)位級(jí)別：碩士專業(yè)：土壤學(xué)指導(dǎo)教師：張小平20080601AbstractFiftyendophyticbacillusstrainsisolatedfrommedicinalplantswerecharacterizedbynumericaltaxonomy，16SrDNAPCR—RFLP，BOXPCRfingerprintsand16SrDNAseque

2、ncetodescribethebiologicaldiversityandphylogenyTheantagonismoftheisolatesagainstpathogenicfungifromcropsweretestedatsametimeThemainresultswereasfollows：Allighphenotypicandphylogeneticdiversityofendophyticbacillusisolated

3、fromMedicinalplantsAndcomparedwithstandardedstrains，accordingtoclusteranalysisofphenotypiccharacter16SrDNAPCRRFLPandBOXPCR，theisolatesweredividedinto13phenons，16and27genticgroupsrespectivelyThephenotypiccharacterizationo

4、fthesestrainsshowedthatmostofthesestrainshadhighstressresistanceAmongtherestrictionenzymesusedAtsplwasthemostdiscriminatingenzymeThegeneticdiversitywashighrevealedbyBOX—PCRBOXPCRfingerprintingisappropriateasamethodreveal

5、inggenetypicdiversitywithinabacillusspeciesandstrainsStrainsbelongedtoonephenoinnumericaltaxonomyalwaysdistributedintoseveralBOXPCRgroupsOverall，theanalysisofphenotypicandgenotypicdiversitywasgoodagreementwitheachotherwh

6、ichapprovedtheresearchresultswerccredibleandstableBasedontheresult，the16SrRNAgeneofrepresentativestrainswerecompIetelysequenced，andthephylogenetictreewasreconstructedTheresultsshowedthatSCAUllWashighlyrelatedtoB勸口erf伽，SC

7、AU78andSCAU25wereidentifiedasBsubtilissubspecies，SCAU39washighlyrelatedtoBmegateriumThephylogenicrelationshipconstructedbythe16SrDNAgenefullsequenceTheresultofantagonistictestsupportedthatSCAUl1，SCAUl0，SCAU49，SCAU33andSC

8、AU46inthestrainstesteddisplayedastrongerantagonisticeffectagainstpathogenicmicroorganism(CurvulariaLunataFusariumgraminearFusariummoniliformeSetosphaeriaTurcicaandSclerotiniasclerotiorum)thantheothersThisstudylaidafounda