中國大豆根瘤菌遺傳多樣性和系統(tǒng)發(fā)育研究.pdf

1、華中農(nóng)業(yè)大學博士學位論文中國大豆根瘤菌遺傳多樣性和系統(tǒng)發(fā)育研究姓名：陳明周申請學位級別：博士專業(yè)：微生物學指導教師：周俊初2002.11.1ABSTRACTFiftytwosoyrbeanrhizobiaisolatedfromrootnodulesof9soybeancultivarsofsoilsamplescollectedfromsevendifferentsitesinChinawerestudiedbyphenotypicc

2、haracteristics，hostspecificity，16SrDNAPCR—R_FLP16SrDNAIGSPCRRFLPRAPD，KEPERICPCRandDNADNAhybridizationTheywerecomparedwithtwentythreetypestrainsrepresentingSinorhizobiumfremJ，Bradyrh&obiumjaponicumandotherRhizobiumtypespe

3、ciesThediversityandphylogenyoffiftytwostrainswerecarefullystudiedTheresultsindicatedthattwentyninestrainswereidentifiedtobelogtoSinorhizobiumfred#andtwentythreestrainstoBradyrhizobiumjaponicumwithinfifty—twostrainstested

4、Theresultsofphenotypiccharacteristics，plasmidpatterns，hostspecificity16SrDNAPCRRFLP16SrDNAIGSPCRRFLPRAPDandKEPERICPCRrevealedthehighdiversitywithinstrainstestedPhenotypiccharacteristicsresultsindicatedthattwentyninefastg

5、rowingstrainscouldbedistinguishedwithSinorhizobiumx#tjiangensisCCBAUIIOandtwentythreeslowgrowingstrainswithBradyrhizobiumelkaniiUSDA76respectivelyButlittledifferencescouldbeobservedwithinfastgrowingstrainsorslow—growings

6、trainstestedTwentyninefastgrowingstrainswereallgroupedwithSinorhizobimnfred／iUSDA205andtwentythreeslowgrowingstrainswereallgroupedwithBradyrhizobiumjaponicumUSDA6andUSDAl10Similaritiesofsomeslowgrowingstrainswererelatedw

7、ithgeographicalsamplingsites，thesimilaritiesofallotherstrainsdidnotcorrelatewiththegeographicalsamplingsiteandsoybeancultivarsAllstrainstestedcouldbedividedbyhostspecificityintotwotypesOnecouldnodulatethesoybeancultivars

8、testedandshowedwiderhostrange，theotheronlynodulatelimitedsoybeancultivarsPlasmidcouldnotbedetectedfromtwentythreeslowgrowingstrainsandtwentyninefastgrowingstrainswerefoundcontaining14plasmidsbyEchardt’smethodThecorrelati

9、onofplasmidtypeandhostspecificitywerereportedResultsof16SrDNAPCRRFLPand16SrDNAcompletedsequenceanalysisrevealedthattwentyninestrainsoffastgrowingrhizobiawereclusteredwithSinorhizobiumfrediiUSDA205andSinorhizobiumxinfiang

10、ensisCCBAUl10Similarlytwenty—threestrainsofslowgrowingrhizobiawerehighlyrelatedwithBradyrhizobiumjaponicumandBradyrhizobiumliaoningensisTherewereonly2bpdifferencesbetween16SrDNAcompletedsequenceofstrainsBAIandBradyrhizob