L-天門冬氨酸聚乙二醇修飾物混合吐溫80-鹽-水液-固萃取體系分離純化肌紅蛋白的研究.pdf

1、中南民族大學(xué)碩士學(xué)位論文L-天門冬氨酸聚乙二醇修飾物混合吐溫80-鹽-水液-固萃取體系分離純化肌紅蛋白的研究姓名：尤偉申請學(xué)位級(jí)別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：沈靜茹20060525L-天門冬氨酸聚乙二醇修飾物混合吐溫 80-鹽-水液-固萃取體系分離純化肌紅蛋白的研究 II Abstract This article summarizes the actuality of protein separation, purification

2、 techniques, and the measurement of protein's content. It presents the structure, capability, and application etc. of Myoglobin. It is complexed a kind of chiral derivant (abbr:PEG- Asp- Cu) b

3、y amino acid (Asp) PEG polyethylene glycol and metal ion (Cu2+). It introduces the interactive mechanism between derivant and heme proteins, and then constructs PEG- Asp- Cu mix Tween 80-inorganic

4、 salt- liquid- solid extraction system. This system is used to do the experiment of Myoglobin separation and purification. Under the best condition, the one- step highest rate(R%) that the system

5、 extracts Myoglobin from horse is 98.57% and the highest distribution coefficient(K) is 102.75; and the extraction of rough Myoglobin from pig's cardiac muscle, the one- step highest rate(R%)

6、 is 85.1%, the distribution coefficient (K) is 103.12. The experiment has constructed two systems: 1. PEG- Asp- Cu mix Tween 80 - (NH4)2SO4 - liquid -solid extraction system; 2. PEG- Asp- Cu mix Tween 80

7、 - potassium phosphate-liquid -solid extraction system. It proves that in the extraction system: the content of PEG, Tween 80, and inorganic salt, the acidity, and the extraction temperature are

8、 very influential. If these had been changed, they will greatly change the partition of Myoglobin in liquid phase and the solid phase. We have found out the best condition of extracting and

9、separating Myoglobin. Under the best condition, if we make use of EDTA and adjust acidity, we can reversibly extract Myoglobin of the solid phase back to the liquid phase. Key words: L- Aspartic