納米金修飾電極在生物電化學(xué)中的應(yīng)用.pdf

1、安徽大學(xué)碩士學(xué)位論文納米金修飾電極在生物電化學(xué)中的應(yīng)用姓名：魯理平申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：無(wú)機(jī)化學(xué)指導(dǎo)教師：金葆康謝復(fù)新2001.6.1交徽大學(xué)申請(qǐng)碩士學(xué)位侖』摘要AbstractItisoneofthe仃endsofchemistrytouseelectrochemicalmethodsinstudyingbiologicalphenomenaProteininorganismisthemacromolecularcontaining

2、severalligands，whichhasimportantbiologicalfunctionApplicationofnanometermaterialshasmadegreatprogressinmodifiedelectrodesNanometergold(NG)particlesexhibitnoteworthyproperties，suchasthesurfacecharge，biocompatiblilityandla

3、rgesurface，etcIt’SthefocusofthisthesistostudythecharacteristicofnanogoldmodifiedelectrodeonthefieldofbioelectrochemistryFirst，wefocused0121“aRentionontheinteractionoftris(1，10phenanthroline)Cobalt(I／／)withBSABovineSerumA

4、lbumin(BSA)wereelectrochemicallyimmobilizedonthesurfaceofnano—goldmodifiedglassycarbonelectrodeTheinteractionofBSAwithTris(110phenanthroline)Cobalt(III)wasinvestigatedbythecyclicvoltammetricbehaviorofCo(phen)3“absorbedon

5、theimmobilezedBSATheresultsuggeststhattheinteractionisstrongAseriesofimportantdatawereobtainedsuchasbindingratioandinteractionmodelandSOonbycyclicvoltammetry(CV)，electrochemicalquartzcrystalmicrobalance(EQCM)andspectroch

6、emicalmethodsToobtaininformationaboutthenanogoldelectrodepositedontheglassycarbonelectrode，XPSspectraweremeasuredCyclicvoltammem7(CV)，differentpulsevoltammetry(DPV)，chronocoulometrywereusedtostudydirectelectrochemistryof

7、cytochromecTheresultsofCVandDPVshowedthatquasireversibleelectrontransferprocessoccurredattheNGmodifiedelectrodeandDNAmodifiedelectrodeWhereaschronocoulometryindicatedcytochromeCwasnotabsorbedonelectrodesurfaceAtlast，DNAm

8、odifiednanogoldglassycarbonelectrode(DNA／NG／GCE)waspreparedbyelectrodepositionTheinteractionofDNAwitlldopamine(DA)epinephrine(EP)andnor—epinephrine(NEP)WasinvestigatedbythecyclicvoltammetricbehaviorofDA、EPandNEPabsorbedo