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1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文乳酸菌自溶影響因素及機(jī)理研究姓名:馮鎮(zhèn)申請(qǐng)學(xué)位級(jí)別:碩士專業(yè):食品科學(xué)指導(dǎo)教師:張?zhí)m威2003.5.1—一,。。。一。,。一,,。:。,,,耋簍童蘭塹蘭墼圭絲i圣;。,一。。,一。。,。。。,。AbstractLacticacidbacteriaiswidelyusedinmanyindustries,especiallyinthefoodindustryTheautolysisofLacticacidbacte
2、riaisimportantIncheeses,theautolysiscanacceleratetheripeningprocessFurthermore,theautolysisofthestartercandestructthefermentingagentandthereleaseofthecytoplasmiccontentsmightaltertherheologicalandorganolepticcharacterist
3、icsoftheproductThefactorseffectontheautolysisandtheautolysismechanismofLactobacillas(LB3)werestudiedThefactorsinvolvedinautolysisaretheincubationtime,theincubationtemperature,saltconcentration,specificmetalionsandpHTheau
4、tolysismechanismincludingtheextractionofautolysins,thesimpleanalysisofzymogram,theautolysinsactivityincellculture,therelationshipbetweencelldeathandautolysinsandtheeffectoftemperatureontheautolysinsactivityThemorphologic
5、alchangewasalsostudiedduringLB3autolysisTheresultsindicatethatthemaximalrateofautol城icactivirywasfoundedattheearlyexponentialphaseofgrowthAtpH4575,therateofautolyticactivitygraduallyincreasealongwithpHAt10—40“C,thedegree
6、ofautolyticactivityincreasealongwiththeimprovingoftemperature,At40“C,themaximalrateofautolyticactivitywasdetectedWithinthefirstfourhours,therateofautolyticactivityat60“Cismaximal,however,duringthelaterdetection,thedegree
7、ofincreaseisnotobvious,InthespecificthreemetalionsWh£ntheconcentrationofCa2is10一mol/L,thestimulatingeffectontheautolysisofLB一3isstrongestTheeffectofNaCIandsodium1actateonLB3autolysiswerealsostudiedtheresultsindicatethats
8、odiumlactateobviouslystimulatetheautolysisofLB3,furthermore,theinfluenceofnegativeionsontheautolysisisratherobviousDuringtheautolysisofLB一3,theclearlychangeofcellmorphologywasfoundedAtcertaintemperature,withinthefirstfou
9、rhours。theindividualmorphologyofLB3hardlychangehowevertheobviouschangewasfoundedattheIaterdectionTheeellofLB3becametransparentandgranulousprobablybecauseoftheintracytoplasmicrecondensationofDNAandtheothersAlthoughthesemo
10、rphologicalchangesweredetectedinalargenumberofthepopulation,notallthecellswerealtered,afactthatmightindicatevariousdegreesofsensitivitytoautolysiswithinasinglecellpopulation。Furthermore,thecellwallhasalreadybeenhydrolyze
11、dbytheautoIysins,losingtheoriginalshapeTheautolysinsystemofLB一3inSDSwPAGEwasshownasthetwozonesThelowermolecularmasseslaneswasdefinedasAzone;thehighermolecularmasseslaneswasdefinedasBzone,ietheautolysinsystemofLB一3consist
12、ofthetwodifierentenzymesystematleastTheresearchoftemperatureandtheautolysinactivityrevealedthattheabilityofresistantheatofautolysinslnAzoneismorethanthoseinBzoneWhenLB一3becomefeebleanddied,theautolysinactivityhasbeenchan
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