腦泰通顆粒對(duì)缺血性腦卒中模型大鼠NGF及IL-6影響的實(shí)驗(yàn)研究.pdf

1、陜西中醫(yī)學(xué)院碩士學(xué)位論文腦泰通顆粒對(duì)缺血性腦卒中模型大鼠NGF及IL-6影響的實(shí)驗(yàn)研究姓名：曹永升申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：中西醫(yī)結(jié)合指導(dǎo)教師：李軍20100401Abstract Objective：To observe the naotaitong granule on MCAO rat brain NGF, IL-6 expression levels, and explore naotaitong particles

2、after cerebral infarction affect nerve growth factor NGF and the inflammatory cytokines IL-6 expression in the role of the relevant mechanism. Methods： Rats were randomly divided into model group and

3、 control group (without occlusion of middle cerebral artery), naotaitong particle group and n piracetam group. By suture method in rats caused by permanent occlusion of the right middle cerebral artery was prepared int

4、o a rat model of focal cerebral ischemia (MCAO). Modeling in rats after gavage once a day. Modeling after 7d, 10d, 14d, 21d, 28d 5 time points rats were killed in batches, remove the brain tissue, using

5、 immunohistochemical method to observe each group NGF, IL-6 expression. The experimental results using the t test. Results：Compared with blank control group, model group, naotaitong particle group and pira

6、cetam group NGF, IL-6 expression was significantly increased; Compared with model group, naotaiotng particle group and piracetam group increased NGF expression; Compared with model group, naotaitong particle group and th

7、e piracetam group increased expression of IL-6, but naotaitong particle group and piracetam group, naotaitong particles in the 7-14 day group was lower than piracetam group ,14-28 days naotaitong expression of

8、 particle group than piracetam group, indicating two-way adjustable naotaitong particles effect. Conclusion：① naotaitong particles can increase the expression of NGF within the brain tissue in order to pro

9、mote the growth of neurons in order to protect the brain tissue. ② In the acute phase of ischemic stroke can inhibit the expression of IL-6 in order to reduce the inflammation of the brain tissue injury. ③ In th