雷莫拉寧的研究開發(fā).pdf

1、上海師范大學碩士學位論文雷莫拉寧的研究開發(fā)姓名：張宏宇申請學位級別：碩士專業(yè)：微生物指導教師：陳代杰李繼安20090501上海師范大學碩學位論文AbstractAbstraetRamoplaninisoneofthecompoundsunderclinicalresearchingusedtotreattheMRSA(MethicillinresistanceStaphylococcusaureus)andVRE(Vancomycinr

2、esistantEnterococcus)infectionsAstrain■ctinoplanessp)，SIPl一A2001，wasusedinthisstudyFirSt，strainimprovementwasmadebynaturalselectionandthefermentationoptimizationwasalsomadeTheresultsofsinglefactorexperimentsshowedthatbea

3、noilandglucosewerethebestcarbonsources，soybeanpowderwasthebestnitrogensourceFilling100mlmediumina750mlshakeflaskwassuitable，thebestinoculationratiowas10％，thebestinitialpHoffermentationmediumwas70，suitableculturaltemperat

4、urewas25“(2Ontheotherhand，feedingL—LeuintothebrothbeforecultivationincreasedthefermentationpotencyComparedtothatofbasalconditionwithoutanyadditionofprecursortheproductivitywasincreasedto150％Thepurificationprocessoframopl

5、aninwasthatafterpreprcessingthebroth，usingmacroporousionexchangeresintopurlfytheproductandusingA1203toremovethepigmentFinalythreepurifiedingredientswereobtainedandtherestructureswereidentifiedbytheanalysesoftheirphysico—

6、chemicalproperties，MS，UVandIRspectradatawhichwereidenticalwithramoplaninAr，ramoplaninA2andramoplaninA3reportedThegeneofD碉5encodingcyclaseoframoplaninbiosynthesiswasclonedanditsgenetictransfersystemwasalsobuildupKeyWords：