MUC4、MUC16及HER2與卵巢癌癌變機(jī)制及臨床應(yīng)用關(guān)系的研究.pdf

1、陸榮MUC4、MUCl6及HER2與卵巢癌癌變機(jī)制及臨床應(yīng)用關(guān)系的研究MUC4、MUCl6及HER2與卵巢癌癌變機(jī)制及臨床應(yīng)用關(guān)系的研究中文摘要目的：檢測(cè)MUC4、MUCl6及HER2在卵巢癌細(xì)胞系中的表達(dá)情況，探索其與卵巢癌發(fā)生發(fā)展的關(guān)系，及其在卵巢癌早期診斷、治療和預(yù)后監(jiān)測(cè)中的作用和意義。方法：對(duì)人卵巢癌SKOV3細(xì)胞株、OVCAR3細(xì)胞株、CAOV3細(xì)胞株進(jìn)行細(xì)胞培養(yǎng)；應(yīng)用RTPCR技術(shù)檢測(cè)人卵巢癌三種細(xì)胞中MUC4、MUCl6及

2、HER2mRNA水平的表達(dá)情況；采用細(xì)胞免疫熒光法及WestenBlot法檢測(cè)人卵巢癌三種細(xì)胞中MUC4、MUCl6及HER2蛋白水平的表達(dá)情況。結(jié)果：(1)RTPCR檢測(cè)結(jié)果顯示：MUC4mRNA在人卵巢癌CAOV3細(xì)胞中表達(dá)量最高，在OVCAR3細(xì)胞中次之，在SKOV3細(xì)胞中最低，差異無統(tǒng)計(jì)學(xué)意義(PO05)；MUCl6mRNA在人卵巢癌OVCAR3細(xì)胞中表達(dá)量最高，在CAOV3細(xì)胞中次之，在SKOV3細(xì)胞中表達(dá)量最低，差異具有統(tǒng)計(jì)

3、學(xué)意義(PO05)，而在SKOV03細(xì)胞和CAOV3細(xì)胞、OVCAR3細(xì)胞和CAOV3細(xì)胞中差異具有統(tǒng)計(jì)學(xué)意義(P005)While，theexpressionlevelofMUC16mRNAWasthehighestinOVCAR3cellline，secondinCAOV3cellline，andthelowestinSKOV3celllineThedifferenceswerestatisticallysignificant(PO

4、05)；andtheexpressionlevelofHER2mRNAWasthehighestinCAOV3cellline，secondinOVCAR3cellline，andthelowestinSKOV3cellline，thereisnosignificantdifferencebetweenSKOV03celllineandOVCAR3cellline，butthedifferencesbetweenSKOV3celllin

5、eandCAOV03cellline，andbetweenOVCAR3celllineandCAOV3celllinewerestatisticallysignificant(P005)(2)TheexpressionofMUC4proteinWasnotdetectedinSKOV3celllineandOVCAR3celllinebyimmunofluochemistryandWesternBlotmethods，butitWaso

6、bservedinCAOV3celllineTheexpressionofMUC16proteinWasdetectedinthreeovariancancercelllinesMoreoverthelevelWashighestinOVCAR3cellline，secondinCAOV3cellline，andthelowestinSKOV3celllineThedifferenceswerestatisticallysignific