多壁碳納米管電極的制作、表征及其對生物分子的電催化.pdf

1、索取號：——旦鰱型丘烈L_n_窯級：—二絲L——丑_—一南京矸芤坶碩士學位論文冒幽多壁碳納米管電極的制作、表征及其對生物分子的電催化。誰j。每“院萊t指導教師：學科專業(yè)：目亞芬化學與環(huán)境科學學院蔡稱心救授∞日化學AbslractFabrication，CharacterizationandApplicationofCarbonNanotubeElectrodeinBioelectrochemistryAbstractThisthesis

2、studiedthefabricationcharacterizationandapplicationsofmulti—walledcarbonnanotube(MWNT)modifiedelectrodeExtensivestudiesweremadeOlltheapplicationsoftheelectrodetodirectelectrontransferreactionofmyoglobin似b)，ferredoxin(SoF

3、d)n蛇electrochemicalbiosensorbasedolleleoholdehydrogenasewasfabricationanditsperformanceswerealsocharacterizedThemainresearchworksareexpressedasfollows：1MbwasimmobilizedOllthesurfac2ofM：WNTandthedirectelectrontransferreac

4、tionwasstudiedCyclicvoltammetricresultsshowedapairofwelldefinedredoxpeaks，whichcorrespondedtothedirectelectrontransferofMb，塒ththeformalpotentialp’)of034340001V(vsSCE)inthephosphatebuffersolution(pn7o)Thedectrochemicalpar

5、ameterssuchasapparentheterogeneouselectrontransferrateconstant(勁andthevalueofformalpotential(∥)wereestimatedThedependenceofp’onsolutionpHindicatedthatthedirectelectrontransferreactionofMbiscoupledwithprotontransferTheexp

6、erimentalresultsalsodemonstratedthattheimmobilizedMbretaineditsbioelectroeatalyticactivitytothereductionofH202and022Spinaciaoleraceaferredoxin(SoFd)，whichwasimmobilizeonthesurfaceofthecarbonnanotubemodifiedglassycarbonel

7、ectrode，couldundergoeffectiveandstabledirectelectrontransferreactionTheresultsofFouriertransforrainfrared(Frm)spectraindicatedthattheproceduresofimmobilization&，F(xiàn)donthesl=LrfageofMWNTdidnotaltertheintegratestructureofSoF

8、dordamageSoFdleadingtoadenaturedstateand&FdretainstheessentialfeatureofitssecondarystructureontheStLrfaceofMWNTn圮cyclicvoltammetricresultsindicatedthattheSoFdMWNT／GCelectrodeexhibitsapairofwelldefinedandnearlysymmetrical