儲糧害蟲體壁上表皮脂類組分研究.pdf

1、河南工業(yè)大學碩士學位論文儲糧害蟲體壁上表皮脂類組分研究姓名：白春啟申請學位級別：碩士專業(yè)：食品科學與工程指導教師：曹陽20070501AbstractTheintegumentalepicuficleofinsectisanaturalinterfacebetweentissuesandorgansinbodyandanenvironmentwhichprincipallyserveaneetoskeletalfunctionEpicu

2、ticleistheouterlayerandissecretedbythehypodermalcellsfirstItisaveryimportantrolethatepicuticularlipidsindifferentfunetiom，includinghydrocarbonsfreefattyacids，freealcohols，sterols，waxes，triaeylgiycorolandtraceothers，sucha

3、spigmentsandhydrophiliclipidsEpicutieularnpidsofinsectintergumentnotonlyhasaroleofphysiologicalandbiochemicalfunctionsforiI略e“butbeappliedtodiscriminateinsecttaxaandresearchanddevelopmentoftheinsecticidesSitophiluszeamai

4、sMotschulsky&oryzae(Linnaeus)，ghyzoperthadominicaFbricimTriboliumcastanetgrB(Herbst)TconfusumDuval，Cryptolestesferrugineus(Stephens)，Oryzaephilussurinamensis(Linnaeus)andLiposcelissppwereextractedbyimmersingthewholebodyi

5、nnhexane(spectrophotometricgrade)under40KHzultrasonicbathwith25士l℃waterandadsorbingLpaetaPearmalI’LbostrychophilaBadonnelDstwinamensis(Linnaeus)andZcastaneum誦thdiatomaceousealth①E)Accordingtoretendontimeforexlracts，Theex

6、ffaetedtimewgresetseparatelywith60min(＆zeama／sMotsehulsky&oryzae(Linnaeus)，zcastaneum(Herbst)，Zconf凇umDural20min(兄dominicaFbricius)，10min(Cferrugineus(Stephens)，Dsurinamemi$(Linnaeus))andlmin(上spp)，andtheepicuticularextr

7、actswerccharacterizedbygaschromatograph／massspectrometry(OC／MS)ThepatternsbetweenDEandepicuticlewereobservedbyscanelectronmicroscopeTheresultsshowedthat：1)hydrocarbonsofepicuficnlarlipidsofmoreagrainpestsforColeopterawel

8、“emaincompoundsandestefswereminorPsoeopteraWerecontrary2)OneorseveralcharacteristiccomponemtsofepicuticularlipidsofstoredgrainpestsCallidentifythestoredgraininsectespecialforthesiblingspecies&zeamaisMotschulskyandSoryzae

9、(Linnaeus)，Zcastaneum(Herbst)andTconfusumDural，Lspp3)ThelipidsdistributionbyabsorbingwithDE嘴similartolipidsbyc】由翟噸cdwithnhexane；4)ThefactorsfortheDEkillinginsectsarehighcorrelativetotheDEgranulesagainsttheintersegmentalf