鼠源多形擬桿菌的分離及其對(duì)小鼠生長性能和脂類代謝的作用.pdf

1、1108015分類號(hào)密級(jí)單位代碼：10019學(xué)號(hào)：S05030471絮目差≮文等碩士學(xué)位論文鼠源多形擬桿菌的分離及其對(duì)小鼠生長性能和脂類代謝的作用BacteroidesthetaiotaomicronIsolatesfromtheconventionalMiceandItsEffectsonGrowthPerformanceandLipidMetabolisminMice研究指導(dǎo)教生：直塑師：張日俊教授申請(qǐng)學(xué)位門類級(jí)別：盔鱟亟向：：塑盤

2、生塹掛盔二Oo七年五月名方學(xué)業(yè)究在專研所中國農(nóng)業(yè)大學(xué)碩士學(xué)位論文第一章目錄AbstractInthisstudyWeadoptedimprovedinediillmfortheselection磺Bthetaiotaomicronandconductedtoinvestigateeffects斌BthetaiotaomicronOngrowthperformanceandlipidmetabolisminmiceExperimentl：

3、InmisstudyWeedup把dimprovedmediumfortheselectionoftheBfra#腦groupfromtimcoloncontentsofconventionalmiceThroughmorphologyphysiologicalandtermentationcharacteristics日：∞ebactedarepremtedspecies研‘BthetaiotaomicronandBvu／gatusW

4、estoredthetwoswainsforalongtimebythemethodofv毫翰珊丘氍耀甜ryin呂andwestudiedeffectsof置thetaiotaomicronongrowthperformanceandlipidmetabolisminmiceExperiment2：Eighty20ageKonmingmicewmrandomlyallocatedintofourgroups(A，B。C，D)with4r

5、eplicatesandeachgroupcontained20miceAWaScontrolgroupBwaslowlevelbaOe—ummodelgroupinwhicheachmousewasiII扛鹋a矧cinfusionampicillin05gcontinuouslyforthreedaysinordertoestablishlowlevelbacteTinnlmicemodelIngroupC20micewereadmi

6、nisteredwithImlAthetaiotaomicronliquidfermentationbygastricinfusiontwiceadayIngroupDfirstweestabliashedthelowlevelb鋤刪岫modelandthenthe20micamodelwereintragastricedthes鋤eba曲涮liquidfermentintlThepurposeofthisexperimentWusto

7、investigatetheBacteriode8伽successioninfourdifferentphysiologicalconditionTheresultshowedthatthegroupBCWIestablishalowlevelbacillusenvironmentanditCanI【e印thissituationfor16daysComparedtothegroupAandCtheanaountofBacteriode

8、singroupBWasdecreasedsignificantly(P005)ThegroupCc∞makethemcc％sioIlalpe酞aheadoftheCommontimeanditCanalsoimprovethepeaknothalf(4％)SuccessionaltrendlineinGroupDcousistentedwithgroupC，butthepeakwaslowerthanthegroupCExperime

9、nt3：Thedesignationoftheexperiment3Wasthesamewiththeexperiment2ThisstudyWaSconductedtoinvestigatethefeasibilityoflowlevelbacteriummodelandstudiedtheeffectsof曼thetaiotaomicronOilgrowthperformanceandlipidmetabolisminmiceThe

10、resultobtainedshowedthatthegroupBhadestablishedtheenvironmentoflowlevelbacteriumwhichcausedgreatsideeffectonthemodelmiceinB，DComp口edt0groupA，thebodyweightofthemiceingroupDwasdecreasedsignificantlyfP《o05)Duringtheexperime

11、ntthebodyweightanddailygainofgroupDwerehigherthangroupB，andintheandoftheexperiment(from28thto34thday)hadarapidlyincreasephaseThephenomenonshowedthat置thetaiotaomicroncouldrevivethesideeffectofantibioticsThebodyweightandda

12、ilygainingroupCwerehigherthanothertreatmentsandthebodyweightWasincreasedsignificantlyfPo05)from19thto22thdayBiochemicalanalysisWasmadeafterbloodsamplewerecollectedandliverandfattissurewerepreservadTheresultrevealedthat丑t

13、hetaiotaomicrontrendedtoacceleratethelipidmetabolisminmiceInconclusion置thetaiotaomicronhadtrendancetoincreasethegrowthperformanceacceleratetheabil姆oflipidmetabolism，andrevivethesideeffectofantibioticsKeywords：置thetaiotao