肺癌患者血管內(nèi)皮生長因子（VEGF）的異常表達及其臨床價值研究.pdf

1、蘇州大學(xué)碩士學(xué)位論文肺癌患者血管內(nèi)皮生長因子（VEGF）的異常表達及其臨床價值研究姓名：吳信華申請學(xué)位級別：碩士專業(yè)：免疫學(xué)指導(dǎo)教師：張學(xué)光姚登福2002.4.1壁墮璺耋皇笪塑生竺堡塑三塑墨堂耋竺墨苧墮墮叢墮旦塞一—————————————————j些!!竺生Abnormalexpressionandclinicalsignificanceofvascularendothefialgrowthfactor(VEGF)inpatients

2、withlungcancerAbstractObjectis，c：Thedevelopmentoflungcal‘cinomaassociatedwithabnormalexnlessionofvasculal‘endothelialgrowthfactor(VEGF)TheailnoftheplesentstudyistOexplorechangesandclinicalvaluesofVEGF。mRNAexpressionandVE

3、GFlevelsintheangiogenesis，metastasisandpl‘ognosisofhumanlungcancelMethods：TheVEGFcellulardistributionsandexpressionin38specimensol、patientswithlungcancel“wereinvestigatedwithimmunohistochemistl’YstaintechnologyThetotalRN

4、Asintissuesof38patientswithlungcancel“waslneasured，thenthelevelsofVEGF—mRNAexpl‘essionwel‘eanalyzedbyareverse—tl’anscl‘iptionpolymerasechainreaction(RT—PCR)assay，andthelevelsofVEGFinseraofpatientswithlungcancel‘，benignlu

5、ngdiseasesandhealthycontrolsweredetectedthroughELISAmethodResults：TheVEGFpositivestainwas76％in38casesoflungcancel“specilnensThe89％rateofVEGFstainwasfoundforclinicalstageIIIcasesand92％forstageIVlungcancersThesignificantly

6、higherexpressionofVEGFwasevidencedinpatientswithlymphnodemetastasis(84％)，distantmetastasis(90％)，andlungcancel‘Swithlowel‘histologicaldifferentiation(89％)，respectivelyTheexpressionleveloftotalRNAwassignificantlyhigherinpa

7、tientswithlungcancersthanthatintheirparacancerousordistantlungtissuesTheVEGFexpressionweretightlycorrelatedwithtotalRNAconcentrationoflungcarcinoma(P001)Thepl’edominantexpressionofVEGF121andVEGF165genefragmentswastbundin